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DAT on Tranfusion Reaction Investigation

BloodbankZ
in Transfusion Services

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comment_78163

I was wanting to get input on DAT's performed for Transfusion Reaction Investigations. Do you perform them with just IgG, C3d or both? TIA.

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  • Malcolm Needs
    Malcolm Needs

    Both.  Not all reactions are caused by IgG immunoglobulins.  Reactions with, for example, anti-Vel can sometimes only be detected by complement coating on the red cells.

comment_78164

Both.  Not all reactions are caused by IgG immunoglobulins.  Reactions with, for example, anti-Vel can sometimes only be detected by complement coating on the red cells.

    comment_78176

    Polyclonal anti-sera (IgG and C3d) on the post-reaction specimen. Positive results then require performance of a differential DAT.

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