David Saikin

You could dilute your IgG sensitized cells to 0.8%. I'd make certain they don't react in the buffered gel card.

The last guidance I could find was from 2018 and was only discussing ways for 5d plts to be extended for 7 days. This was going to the BPAC for their input. In 2016 their was a guidance for comments which would require bacterial contamination testing for plts on day 4 or day 5 if the product was in your control and was going to be transfused. This guidance was put on hold the last I knew (which was also in 2016). I'll see if I can find any further info.

I use immucor's CorQc for my positive qc; dilute the antibody 1:10. You can use the AB+ cells - you should replace the diluent w Ortho's. For negative I make suspension of O= rbcs and I use the diluent as my negative (checks both cells an diluent for contamination).

If you are using the polyahg card you should qc it w IgG sensitized cells and C3d sensitized cells. Many places only use the IgG cards, so I would think your daily qc should suffice.

You may be using a monoclonal anti-K which will work in the neutral/buffered gel cards. Whereas, as the above posts state, the patient's anti-K will require an indirect antiglobulin test to detect.

with our ordering scenario, the MD can provide the order to transfuse w the orders for components. I do not f/u on orders, we do not have access to the MD order set. The only time I call is to the OR. Everything is electronic.

you would have to dilute your qc ab quite a bit. I know when I started gel CAP required absc cells to react 1-2+. Now they just have to react. I use a 1:10 dilution of my antisera to qc reagent rbcs in gel.

that standard (5.11.4), I believe, is directed at rbc transfusions. I do not keep a specimen for an FP or plt transfusion.

I would anticipate that you would be expecting the DAT to be negative. HOWEVER, I have seen a mother w an antibody to a private ag of the father. Baby's DAT: 4+. Mom's ab screen was negative. Sent to NY blood center: they could not identify ab either. As I said, private antigen from father.

Make certain to validate the interface between EPIC and your BBIS. I noticed a 30 minute delay between them when HCLL and EPIC went on line together. Could never get to see the interface validation (I was a temp manager).

If your centrifuge has a brake and it is in use this: could be causing your delineated button problem.

You should have some standard operational things to review annually. Plus, there probably are issues that arose during the year that could also be discussed. In your Quality Plan you should have some reports that are due during the course of the year. Make certain you have them and that they have been reviewed by your quality team.

typically you are going to make modified whole blood w a hct of ~50% (your neonatology folks will give you the target hct they want - this should be standard and not subject to the MDs whims at the time). You will need the average hct of a unit of stock rbcs (and you will use this number all the time, unless you plan on doing a hct on every unit you are going to use). A sterile docker is not essential but will allow your product to have more than 24 hour exp date. You should be able to discover the formula for adding plasma (to get the desired final hct) in the Technical Manual.

If we are giving unxm'd rbcs to a pt with an unknown type, O Pos unless a female of child bearing potential (up to age 50 for us).

the wording is the same in the 31st edition. I have to agree with you about a current specimen. As I stated above, we get a current specimen and it is good for non-red cell transfusion for the duration of the admission.