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David Saikin

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Everything posted by David Saikin

  1. Run some panel cells; negative, heterozygous and homozygous.
  2. Wish I could offer you some experience w these patients. Currently we've only had one positive pt and that individual was not admitted. We don't anticipate component usage for these folks but you never know.
  3. Drucker says they used to make those for CA. Their serofuge has a different body but the same rotor and guts. FYI.
  4. If you have done the alloabsorption, do you not elute and test the absorbed abs? I find it hard to believe that a patient would be sensitized to all the ags except C and E. (The most abs I've ever encountered were 10).
  5. That's up to you and your Medical Director. One thing to consider - that would be a lab developed test. I thought I read a few years ago that the FDA was going to look at those (LDTs) - I think there was going to be a $250,000.00 fee (the initial fee for them to evaluate proposed new tests/drugs). Also, when the Orthos and Immucors validate their antisera I believe they run considerably more than the "20" samples (including variants), so, while you may validate your process I don't believe your validation is as extensive, esp since you will be using reagent "off label". I'd be more concerned with the LDT. I'd check w the FDA to see their take on your proposal. FYI - I've used gel off label for years w antigen typing (and a few other assorted tests).
  6. That's the truth John. Us small places are at the mercy of the blood suppliers.
  7. David Saikin

    serofuge

    Anybody use Drucker Diagnostics SERO 12 blood bank serofuge? If yes, what is your opinion. Thanks.
  8. No offense at all. Who knows if I do things the way the ARC does them . . . or even AABBs "modern method", which to me is a serial 1:3 dilution (1 drop cell suspension and 2 drops test plasma/dilutions)- though that is read microscopically. I don't save send out titers as we do them routinely anymore here.
  9. Original specimen titer was 2. Monthly. Went to 4 and then 8. (Red Cross did the first 2, I did the last as Titer was not ordered so phlebs only drew a small lavender. ARC wants a lot more than that, as they do another abid, r/o G, and a few other things in addition to the titer ordered.
  10. My thoughts exactly. I don't know how far along - I'm guesstimating this was her 26 week antenatal experience.
  11. I have this prenatal patient who presented with a weak anti-D,-C (G ruled out). G4p2. Her initial D titer was 2 f/u with 4 and 8. After her 3rd titer she was given RhIg in the office. My question: are further titers a moot point? The anti-C was not titerable initially and has been undetected in subsequent samples.
  12. if I sign out emergency release, I am keeping the request regardless if rbcs are used or not.
  13. Those are probably chemistry/hematology analyzers. They do the same here in the USA - for those lab areas. The FDA has guidance for computer validation - it recommends making your own validation studies based on how you will use the BBIS. I would anticipate that the same will be true for BB instrumentation.
  14. Did you test your A2 cells in gel or in tube? If in tube, did you look at it microscopically?
  15. Does look like a AsubB w anti-A1. I'd test pt cells w A1 lectin. Also run a small IS/rt panel of screening cells, A1, A2, auto cells and see what it looks like. By running the screening cells along with A1/A2 cells I can get an idea if it is a cold specificity or anti-A1. a
  16. We have a successful blood drive every 8-10 weeks. The problem, as I see it, is that the ARC does not understand the small hospital BB situation. We are at least 2 hrs from our overstock and 3 hrs from the Red Cross distribution site. My feelings are that the 7 small hospitals in Northern NH and Vt should be receiving units w the best outdates. After 2 weeks we should be able to ship them all to institutions who use significantly more products per week than the 7 of us combined do. Anyway, I don't want to go on a diatribe, suffice it to say that I think the rural hospital blood banks are not understood by the major suppliers. I can go 2-3 weeks without a transfusion and months without thawing plasma. Unfortunately, when my need is critical, usually my inventory levels are too (at least O+ and O=).
  17. The probe on my plt incubator is out in the open. I actually have placed it in a 5cc syringe w DI H2O. Knocks the sensitivity down a bit which is good. I take the probe (in the syringe) and place it in an ice water bath and a warm water bath. I document the temp when the alarm sounds. This allows the chart to also document the temp checks. I also document the internal thermometers with the NIST standardized one. It is almost impossible to get the NIST therm into the liquid with the probes.
  18. you can place the fluid w the probes in a container of warm water. Temp should rise rapidly and you'll get the spikes on your chart.
  19. it reacts strongly. +w would mean weaker reactivity. (due to ag density on the particular cell I believe)
  20. definitely doesn't look like Sda or Lutheran abs. The auto being negative at ahg is what caused me to be confused (I hope).
  21. Can't answer that one John. From the ER.
  22. I can go for months without using plasma so I keep my bath empty until I have orders. I fill it w tap water
  23. I use the smaller (4u) Helmer product. Works very well.
  24. I understand but in a 20 bed hospital it works just fine. You also run into the issue which perplexed me for a while. Weak gel rxs are invariably negative in tubes no matter what enhancement you use.
  25. Repeated DAT in Gel 1+w. Makes me feel better. Cannot resolve the reverse grouping results. I played with both specimens (as did the evening folks). The mf results in gel were pretty equal as to top and bottom. I only did prewarmed w PeG so no immediate spin. Elution is a 4+ panagglutinin in gel. Doesn't look like rouleaux. I think the Peg absorptions work nicely (have done many).
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