Transfusion Services

Hi all, I'm a fairly new tech and I have a question regarding testing methods on patients that are actively on DARA. My hospital currently uses gel as its primary testing method. DARA patients, of course, are panreactive 1-2+ throughout both in the ABSC and the ABID panel. My current policy is to perform a DTT-treated screen and treat units if they need a transfusion. We provide K matched units and perform AHG XMs on treated RBCs. Many of these patients are surprise patients and there is frustration from providers when we tell them that the work up will take a few hours. I never want to jeopardize patient safety, however, I'm wondering if there is a mor…

Has anyone seen the antibody screen become completely negative after a patient was transfused with plasma and platelets. We saw a patient the other day in the ED with positive screen and Anti-FYA. Received uncrossmatched blood and plasma and was transferred to another facility. Later they performed a type and screen on the patient and it was completely negative. Both our facilities use solid phase testing. Wrong blood in tube was ruled out. Thank You

Please looking at malaria testing prior to transfusion especially in endemic areas like in Africa, in Europe someone coming from endemic zones are usually deferred from donating for a period of time but here in Africa during transfusion some centers will screen for malaria and some will not screen. The first question will be, it's necessary to screen for malaria in endemic areas before donating blood or not screening knowing knowing half or more than half of the population can test positive for malaria? and the second question what is the better tool for screening microscopy or rapid diagnostic test using serological cassette? Going to be grateful with feedback 🫲🫲🫲

Hi, I would like to get rid if ortho reagents antisera and red cells to replace with immucor reagents.. how many samples are needed and how many aborh,dat,etc to validate this immucor reagents. Please share your procedure. Thanks

Pt is 50 y/f on ECMO A+ AB scene negative. We are on day 5 or 6 and she is hemolysis lime crazy. I remember something about hyper hemolysis syndrome on this site. Recheck AB screen still negative DAT is negative. LDH super high, sorry I was off a couple of days got called at home about this case. I’m writing this on my break and have to go back and work on other patients. Will try to respond when I can. Saline used was 0.9% . Patient also has impella device which we’ve seen hemolysis with but not like this. This looks like intervascular hemolysis.

Can immediate spin be eliminated for cold agglutinins? We are going back and forth with this because immediate spin detects ABO incompatibility but the computer system is also set up to detect this.

If a small site that doesn't stock irradiated blood has to give emergency transfusion or a bigger place can't provide several rounds of an MTP that are irradiated, what are the risks? I know that patients who had a Stem Cell transplant in recent years must get irradiated products or face a very dangerous form of GVHD. I know that patients on purine analog drugs should get irradiated blood, but I don't know how dangerous it is if they don't. Otherwise, most patients seem to get irradiated because they are candidates for future transplants. Clearly, we can't let patients bleed to death but, if we get an MTP order on someone our system says needs irradiated blood, what do…

Over the weekend the overnight shift received a Type and Screen, later the patient received blood. They did get a positive screen, but they were able to rule out all antibodies. When I followed up on the patient, I found out that the patient had history at another hospital in the area, not in our system. When I called the hospital, the patient actually had received blood at that facility earlier this month and had developed Anti-JKb and little c. Obviously, blood we gave to him was not tested for those antigens. Is that an FDA reportable error? This is first time something like this happened with me as a supervisor. I notified the patient's care team, immediately. Other…

Looking at those conditions listed above, cancer, bleeding conditions, heart and lungs conditions which sometimes can pose a serious problems with the donors when donating blood and to a less exend may affect the recipients. How can such conditions be identify in donors who have never be diagnosed on such conditions and might be having it and don't know they're having it? What can be done to rule out these conditions? Thanks

Hi, I am currently working at a facility which stuffs its BB refrigerator shelves to the gills with pRBCs. They are nut to butt, like sardines in a can. My instinct says this is not appropriate storage, and maybe something about not enough air circulation? However I'm drawing a blank on which agency might have a guideline or standard about this, if there are any. Any help would be appreciated!

Greetings to everyone. Please like to get responses on this inorder to have a bigger picture. In my country mostly what's done is just the forward grouping using the anti-sera to do and confirm the patient blood group and when that's done they just sent out of the hospital to collect blood in a donor centre ( laboratory) and in the donor centre sometimes what's done in still the forward Grouping without doing the reverse blood grouping. The question will look like this, what's the importance of always doing the forward and reverse blood grouping before transfusing blood? Or even when the hospital do the forward grouping it's advisable for the laboratory to do the reverse …

Does anyone allow electronic crossmatch to be used in cases where a prior marrow transplant will never result in the usual reverse type on the patient (for example B to A)? We have a discrepancy blood type test in our computer that doesn't require the usual truth tables be met that we currently use for these patients. We require 2 separate specimens to be typed before we give any RBCs that aren't group O. We require 2 individuals to review any results turned out using the discrepancy test (this doesn't mean mistakes couldn't happen). We could have a patient with a cold agglutinin that would make an IS crossmatch incompatible (although maybe immunosuppressed patients w…

We have patients that come home from university hospitals after transplants that we need to transfuse. We can often find in their charts the recommendations of the transplant center on what donor types to use. When we can't, I would like to have a definitive source on what blood type we should transfuse to these patients. The current patient was B pos, now A pos after a transplant 6 years ago. His chart says they consider him "cured". He forward types as A and back types as AB, as expected. I think we should be giving him A pos RBCs but I want a definitive source to cite in his notes.

Anybody Have written plan for Echo Validation please.Thanks

I have been searching for support reference material for 2 people to issue blood products. All I can find is 2 people at time of transfusion. I looked in the CFR and cannot find what I am looking for. Maybe I am not looking correctly. Maybe we don't need 2 people to issue blood products. Can anyone direct me to a reference or inform me on the correct process for issuing blood products? pretty please

Hi we are currently in the process of implementing ortho vision analysers across our health board in the UK. I was wondering if anyone who is currently a user of the instrument would mind sharing their SOP with me? Many Thanks.

Sorry if this the wrong Thread. Also, I apologize for this dumb question. What does +s in the ortho Panel mean? Thank you

Good morning! I wasn't sure which section was the best for this discussion but hopefully people will find it here. I'm over training/education/competency for our market which includes 15+ facilities ranging from stand-alone ED to a level 1 trauma center that acts as a reference lab for all of the other facilities except one. We are trying to develop one system that would work for us all. So, my questions/topics for discussion: 1. Do you perform an initial competency at the end of training or as part of training? 2. Does each test system have its own checklist for training? Ex.: Automation, ABO/Rh, use of irradiator, weak D, etc 3. Do any of you h…

We have a 42 y.o. Caucasian female with chronic anemia and cellulitis/sepsis needing debridement who has anti-D (2+) & anti-C (3+) by Ortho MTS gel. She was transfused elsewhere in 2021 and here in 2022, all Rh neg units. Two units each time. Screens were negative then. She has a history that suggests she may have shared IV drug needles at some point. I don't think there is a pregnancy history but not ruled out. She is A neg. Her initial testing in Ortho gel was clearly anti-D with C (could include G) but she had some 1+ reactivity with 4 of 5 D and C negative panel cells. The cell that didn't react was E+, D-, C-. Fya+, Fyb-, heterozygous for Kidd and MNS, but Lea- …

We need to determine how we will issue cold stored platelets in a massive transfusion. We would like to put them in coolers packed like RBCs, but we found that they get too cold. Has anyone identified a packing method that works well for CSP but doesn't mean we have to annually check 16 coolers by two different packing methods so we can use them for either CSP or RBCs? We may have to dedicate some coolers to platelets but would rather not.

Hello, Our lab recently acquired the Barkey Plasmatherm. To comply with IQMH requirements, we need to check and document the thawing device's temperature at each use, ensuring it stays within an acceptable range. However, we’ve noticed that the thawing temperature sometimes exceeds 37°C. According to AABB guidelines, plasma should be thawed immediately after removal from storage, either in a water bath at 30-37°C or using an FDA-cleared dry thawing device. My question is: since the Barkey Plasmatherm is an FDA-cleared dry thawing device, does the 30-37°C requirement still apply? Thank you!

I feel like the FDA requires that forms be controlled documents, but I can't find a regulation stating that. AABB includes forms in their definition of documents, but I can't find anything similar in the FDA regulations. FDA used to have a Guidance for inspections, but I can't find that either. Any pointers appreciated.

What would cause these results? I don't expect the A1 lectin testing to look like the other tube testing in most weak subgroups that I know of. I also thought maybe the reaction with anti-A,B would be stronger. We are still getting answers to whether there may have been a marrow transplant. The leukemia question was about suppressed antigen expression. Chimera? What else? Patient is a pregnant Native American. I assume first trimester because this is initial prenatal testing.

CAP standard TRM.40130 is a new standard that deals with alternative control procedures. It states "If the laboratory performs test procedures for which control materials are not commercially available, there are written procedures for an alternative mechanism to detect immediate errors and monitor test system performance over time. The performance of alternative control procedures must be recorded." We use the Ortho Confidence system for gel. We also use AB Plasma (we aliquot one unit of expired FFP and use it until gone) for our reverse type in confidence cell 1, to test the buffer part of the card and also to test our screening cells in the IGG card to make sure t…

Anyone else using Vitalant? We do partially. Wanted to know if you've gotten any information on how frequent this low-yield supply will be? I get why they're supplementing inventory with these, but I'm having trouble getting information from my account rep. This involves a lot more than just turning on product codes for us, so putting out feelers. We're so remote I don't have a good network of other users.