Jump to content

Malcolm Needs

Premium Members
  • Content Count

    7,554
  • Joined

  • Last visited

  • Days Won

    738
  • Country

    United Kingdom

Malcolm Needs last won the day on March 19

Malcolm Needs had the most liked content!

About Malcolm Needs

  • Rank
    Seasoned poster
  • Birthday 12/14/1954

Profile Information

  • Gender
    Male
  • Interests
    Rugby Union, Cricket, cooking, wine, port, reading, crosswords, lecturing, more wine and more port!
  • Biography
    Pretty boring really, but not that pretty!
  • Location
    Milverton, Somerset, England
  • Occupation
    I have taken a brand new role in the NHSBT and am now involved very much more on the education and training side of red cell immunohaematology. My title is still Reference Service Manager, but with Training after it (Reference Service Manager - Training). I am very excited about this change, as I have a passion for training and education.
    Reference Service Manager with the NHSBT.
    Chartered Scientist.
    Fellow of the British Blood Transfusion Society, having twice served on their National Council.
    Fellow of the Institute of Biomedical Science. Member of their Special Advisory Panel for Transfusion Science and Chief Examiner for Transfusion Science for the Institute.
    Author of the chapter "Human erythrocyte antigens or blood groups" in Fundamentals of Biomedical Science, Transfusion and Transplantation Science, edited by Robin Knight, for the IBMS. 1st edition, Oxford University Press 2013 (ISBN 978-0-19-953328-2, pages 19-44.
    Just been appointed to the BCSH Blood Transfusion Task Force (writing Guidelines).
    Member of ISBT and AABB
    I am now retired from the Blood Service, but still do the other things!
  • Real Name
    Malcolm Needs CSci FIBMS FBBTS

Recent Profile Visitors

12,832 profile views
  1. Yes. All changes have to be validated.
  2. Thanks for those suggestions, but PLEASE, don't call it anti-Kell and anti-Cellano!
  3. True, but, in the early days, I know of at least one example that caused almost pure red cell aplasia in a BMT from a group A donor, because the contaminating anti-A was so strong.
    1. Malcolm Needs

      Malcolm Needs

      No, I hadn't, but I am not surprised that ABO antigenicity is thought to make a difference.  ABO antigenicity makes a difference with several pathological conditions.

    2. helalami
  4. Phew, that's a difficult one! You could begging some from one of the world's big organisations, such as the International Blood Group Reference Laboratory in the UK, somewhere like the Mayo Clinic in New York, Amsterdam, South Africa, etc, but other than that, I don't know. Some of the large reagent suppliers, such as Ortho or BioRad may possibly have some in store too.
  5. I must admit, we always used to use polyclonal antibodies, and there is a reason for this. Even blended monoclonal antibodies will still only recognise certain epitopes within the antigen, and while the K and k antigens tend to be single amino acid substitutions of methionine at position 193 for the K antigen, and threonine at position 193 for the k antigen, this is by no means universal. A weak expression of K is seen when either an argenine or a serine residue replace the methionine residue at position 193. As with amino acid substitutions leading to weakened expression of the K antigen, so the same can happen with the k antigen, but these substitutions may not actually be at position 193. A recent publication has shown that a substitution of Leu196Val weakens the expression of the k antigen (see Uchikawa M, Onodera T, Tsuneyama H, Enomoto T, Ishijima A, Yuasa S, Murata S, Tadokoro K, Nakajima K, Juji T. Molecular basis of unusual Kmod phenotype with K+wk-. Vox Sang 2000; 78 Suppl. 1: Abstract O011, Poole J, Warke N, Hustinx H, Taleghani BM, Martin P, Finning K, Crew VK, Green C, Bromilow I, Daniels G. A KEL gene encoding serine at position 193 of the Kell glycoprotein results in expression of KEL1 antigen. Transfusion 2006; 46: 1879-1885 and Millard GM, Lopez GH, Turner EM, Lizarazu ME, Roots NM, Liew Y-W, Flower RL, Hyland CA. Modified expression of the KEL2 (k) blood group antigen attributed to p.Leu196Val amino acid change three residues from the K/k antigen polymorphism site: implications for donor screening. Transfusion 2019; 59: 1156-1158.). Therefore, it is more likely that you may get a false negative result using a monoclonal antibody in adsorption/elution tests, than by using a polyclonal antibody, with its wider breadth of specificity to the antigen's epitopes.
  6. I can see no reason why this would not work as a method. We used to use something very similar when testing individuals expressing the "McLeod" phenotype. However, these days it would be easier (and certainly more accurate) to perform gene sequencing on both the KEL and the XK genes. Don't forget that a true Duffy null phenotype (FY/FY), as opposed to a "GATA-1" type Fy(a-b-) is, these days, established by molecular techniques.
×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.