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BloodbankZ

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    United States

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  • Occupation
    Blood Bank Coordinator

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  1. BloodbankZ

    Stat Centrifuge

    We use Ortho gel primarily. It states nothing about centrifuge time. We use greiner tubes and they recommend 2000g for 10 min for platelet poor plasma. I am leaning towards the Stat spin express which is 3 minutes at 4000g. As long as it validates I don't see a problem do you?
  2. Check EBAY I picked up a Clay Adams Serofuge there to "revitalize" one of ours. I think it was only $400.
  3. BloodbankZ

    Stat Centrifuge

    BankerGirl do you know the g force?
  4. BloodbankZ

    Stat Centrifuge

    I am looking to cut down our TAT. We currently spin our specimens for 10 minutes. Any suggestions for stat centrifuges? TIA.
  5. I am needing some help setting up electronic crossmatch in Meditech. I have everything else in place but can not figure out how to prevent EXM in patients who have mixed field reactions. I am wanting to be able to perform the IS serologically as I understand this is needed per CAP and FDA regulations. Has anyone else experience this. We are currently on version 6.08. Thanks in advance!
  6. I would say it is due to the DARA. DTT treated cells will eliminate the DARA reactivity and let you be able to rule out everything besides mainly Kell system which DTT denatures. We perform a baseline type and screen and DNA HEA typings on patients before they start. If DTT screen is negative we give K negative cells if patient is antigen negative.
  7. When I tried to run it in gel I never could get nice clean reactions. Their would always be particulate at the top of the wells. I have seen several tube procedures that are just using so many drops of 4% cell suspensions instead of concentrating their cells and then getting an exact 4:1 ratio with DTT. I have since went to doing it in tube. What kind of steps are you performing?
  8. BloodbankZ

    FDA reportable events

    Thanks so much Eman that is just what I needed. I just couldn't find it.
  9. BloodbankZ

    FDA reportable events

    I was wondering if anyone knew of a report that showed incident rate of FDA reportable events per hospital or per other statistic like transfusions, patients, etc... Just looking for a baseline to see how our facility compares to others. I have searched and reached out to other facilities and haven't had any luck. Thanks in advance.
  10. Has anybody tried DTT treating reagent red blood cells in MTS gel method? If so are you concentrating your cells before treating or have adjusted your ratio of DTT to the .8% suspension? TIA.
  11. What are other people's institutions practices on the following. If you have a patient with an anti-D do you need to go ahead and carry out the D antigen typing on the patients rbcs through the IAT phase(weak D testing)? The AABB 18TH ed. Technical Manual states on pg. 327 "When the D type of a patient is determined, a weak D test is not necessary except to assess the red cells of an infant whose mother is at risk of D immunization." It then goes on to say under Identification of Antibodies to Red Cell Antigens pg.401 "Determining the phenotype of the autologous red cells is an important part of antibody identification." We use MTS gel for as our primary method for blood type determination and it states that Most weak D antigen expressions will be detected(which means not all), however partial DVI epitope variant of the D antigen will not be detected with this monoclonal reagent. Not that it really changes how we transfuse the patient but just curious to others procedures/thoughts. Thanks in advance.
  12. Did you ever figure this out I am trying to get the same thing done with no luck.
  13. BloodbankZ

    Ortho MTS Gel Pipette

    We have been using Ortho Gel since 2008 we have serviced our Tipmaster pipettes several times. We are needing something new has anyone used the Vista Ovations for Ortho Gel or have another suggestion. Thanks.
  14. BloodbankZ

    Rule of 3

    Okay so I was always taught to use the rule of 3, 3 positive reactions and 3 negative reactions for peforming an antibody ID. I was also taught to always use homozygous positive and negative cells whenever possible. Sometimes of course it is not due to low incident/high incident antigens. I do know you need to use a homozygous cell when performing "rule outs". What is everyone else's practices and thoughts as I need to clarify our current antibody identification policy. Thanks in advance.
  15. BloodbankZ

    Issuing multiple units to one patient

    To clarify if we do issue more than one unit they are placed in a cooler with appropriate temp control. Also our coolers are validated for 24 hrs.
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