Malcolm Needs
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Everything posted by Malcolm Needs
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Issue for the Identification of Antibodies
Malcolm Needs replied to gebae's topic in Immunohematology Reference Laboratories
I would treated the patient's own red cells with papain or ficin (whichever is used by the manufacturer to make their enzyme treated red cells), and then test them against autologous plasma. My guess (and from this far away, it is just a guess) is that they will be positive. I think that there is a "cold" reacting auto-antibody there. I would also suggest performing an IAT panel in tubes, bringing the reactants to 37oC before mixing them, and using isotonic saline, rather than LISS as the red cell diluent, and then washing the tests in pre-warmed isotonic saline. This should show i -
Welcome epannell.
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Welcome gebae.
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Welcome Rosalie Pagunsan.
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Welcome LabLady.
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Not in the UK.
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Welcome desilu0.
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BloodBankTalk:Blood Transfusion Therapy in Haemoglobinopathies
Malcolm Needs replied to Cliff's topic in Question of the Day
Well done Arno! -
Welcome Leslie Tech.
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Welcome Mark Kerr.
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Welcome s.marques.
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Macroscopic every time.
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Welcome Candice.
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Welcome mjkealy.
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Welcome Yen.
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WOW!!!!!!!!!!!!!!!! THAT really is so scary!
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Welcome mbrown.
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I think you would also need to re-word your comment slightly, as your comment, as written, is not valid in all countries. Within the BSH Guidelines for pre-transfusion compatibility procedures in blood transfusion laboratories (Milkins C, Berryman J, Cantwell C, Elliott C, Haggas R, Jones J, Rowley M, WIlliams M, Win N. Transfusion Medicine 2013; 23: 3-35) states, in paragraph 4.3.2 D typing, "i Where secure automation is used, D typing may be undertaken using a single IgM monoclonal anti-D reagent, which should not detect DVI. In the absence of secure automation, each sample should be
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For all I have said above, and I think I have said this before on here, when I was first working in Red Cell Reference, when the International Blood Group Reference Laboratory was in London, and the Department was run by Carolyn Giles and a very young Senior Technician by the name of Joyce Poole, I also had the problem of seeing "weak agglutination" that wasn't actually there (totally negative, in other words), and Joyce coined this as a "Malcolm Weak". This was way back in the early 1970's. I understand that, now and again, some 50 years on, the term is still used in the department for ove
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Within my own laboratory (Immunohaematology Reference Laboratory NHSBT-Tooting Centre), one of my senior staff, Alan Gray, was the designated member of SCARF. Unfortunately, Alan has retired and, as I understand, is now in poor health. I will try to see what has happened, but do not want to cause him any difficulties.
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Sorry, my apologies - wrong end of the stick!
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Have you thought of hiding his glasses?
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True, but, for those unfamiliar with it, the agglutination seen with an anti-Sda is usually mixed-field (see the attached photograph - not a fantastic photograph, but I have "tarted it up" a bit - from the original paper, Macvie SI, Morton JA, Pickles MM. The Reactions and Inheritance of a New Blood Group Antigen, Sda. Vox Sang 1967; 13: 485-492).
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Fine pbaker. No problem with that, but there are some places that, even today, check EVERY apparently negative reaction with a microscope.
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It isn't. We used it whenever we were looking at a possible ABO HDFN in the Reference Laboratory at NHSBT-Tooting Centre.
