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  1. Yesterday
  2. Documentation of Transfusions

    We were cited on the 30 minute rule at our last CAP inspection. We now rely on the temperature of the unit, taken with an infrared thermometer, if the unit is returned. The unit must register < 10C to be acceptable back into blood bank inventory. And we see, too, that the temperature can be out of range within a relatively short period of time.
  3. UKAS - Uncertainty of measurement in BT

    We were inspected recently and did not have any UoM for Diamed systems, which they had no issue with. We do however have a table in the SOP listing mitergation of uncertainty for the analysers but no actual calculations and they were happy that we had considered it. I think in the standards it says to 'consider' uncertainty so we listed things like IQC, EQA, competency assesment of users, 3rd party controls, standard reagents that have a BAT etc.
  4. Post Vasectomy Sperm Presence

    Did you try a hemocytometer? It seems like a count of less than 100 / ul would be doable on one. See: http://www.vivo.colostate.edu/hbooks/pathphys/reprod/semeneval/hemacytometer.html (you can find anything on the internet!) Scott
  5. Silica-Coated Tubes for Testing

    I don't know if/how silica might affect testing, but I think that might only apply to the stopper, not the tube proper. You could certainly use regular 16 x 100mm (borosilicate) tubes, rather than the "vacuum" versions. We get generic tubes from VWR, cat # 47729-576.
  6. Hello all, We are looking for larger tubes than our normal 12x75 tubes to use when performing adsorptions. I'm finding that a lot of serum glass vacutainer tubes, that would be the perfect size (16x100), are silica-coated. Would this silica coating interfere with any of our adsorptions or post-adsorption testing? Thanks!
  7. We currently perform post vas sperm presence and report our results as number of sperm seen per HPF and make a note if the sperm were motile or non motile. The American Urological Association states that a vasectomy is successful if less than 100,000 sperm/mL are seen. Does anyone have any idea how to convert sperm per HPF to sperm per mL? We could use a hemocytometer but numbers are usually so low that doesn't seem logical.
  8. For Type and Screen we use ambient 1 day and refrigerated 3 days.
  9. Specimen Collection and Processing

    In general, our procedures require a specimen up to 48 hours old, as long as it is stored refrigerated. Usually specimen testing is begun soon after the specimen reaches the blood bank, where they are left at room temp while that is completed. Scott
  10. What is everyone's requirements for specimen collection and handling for pre transfusion testing? I am having a hard time finding out how long specimens can sit at room temp. How long specimens can sit unspun. ETC. Thanks for any info or insight
  11. B(A) Phenotype?

    While I admire your tenacity in following up your case Kellimq, unless your R&D Rh genotyping facility is well-practiced in ABO genotyping ( and I am NOT saying that they are not), you should keep in mind that ABO genotyping is notorious for predicting ABO phenotypes that do not necessarily match the genotype (see Daniels G. Human Blood Groups. 3rd edition, 2013. Wiley-Blackwell, p25, Section 2.3.2.6 Predicting ABO phenotype from DNA testing.). Certainly, in the UK, our Histocompatibility and Immunogenetics Laboratories, who used to perform ABO gentyping when performing the tests for renal transplants have been banned from so doing, and now have to use good old-fashioned serological techniques, are one unfortunate episode.
  12. B(A) Phenotype?

    Hi, We recently also had a B (A) phenotype detected by the Ortho reagents. Our reactions were similar to yours. We are fortunate to have a R&D Rh gentoyping facility at our Reference Lab here in Brisbane, Australia who confirmed the B (A) phenotype. I confirmed with Ortho they are using the clone which detects this. We transfuse Group B where possible.
  13. Last week
  14. Just saying Hi

    I got bored watching day time television and so, after I had brought my lectures up-to-date, and taken them off the blood service's PowerPoint background, and put it on my own, I decided to become a locum, and Zoe is now my boss for a while.
  15. base line pretransfusion vitals

    My only concern with pretransfusion vitals was to give a baseline in case there was a change during or shortly after the transfusion. I'm curious as to why you are aware of the pretransfusion vitals? I would certainly hope the nurse is aware and not in need of being alerted.
  16. TRM.42750 Storage Unit Alarms

    Interesting, I don't remember ever seeing a freezer that even had a low temp alarm let alone a way to check it! I think we just figured that part didn't pertain to freezers.
  17. Just saying Hi

    I thought Malcolm was retired!!!! What's this boss stuff?
  18. wAIHA with IgM and C3c/C3d coating

    Sadly, I doubt whether this was done by the NHSBT RCI laboratory. Such testing is usually deemed "too expensive" under "LEAN" rules!
  19. Lewis A

    No Anna.
  20. wAIHA with IgM and C3c/C3d coating

    OK Malcolm, in that case you can exclude it. I hadn't noticed the little English flag on the first post! Has anyone thought to do an eluate? That might be helpful
  21. B(A) Phenotype?

    Or, more mundanely, the reaction in the anti-A could be down to carry over. When working with gel, if there is condensation in the reaction chamber, or even worse, directly under the aluminium, this can be carried over when pipetting or when removing the aluminium. As the antiserum is so strong this can lead to false positive reactions - rarely a 4+. Is it too late to see a picture of the pipetted card?
  22. Lewis A

    Malcolm, are you really trying to kill Mr. Lewis??????????
  23. Lewis A

    From you to our pathologist's ear, Malcolm! Scott
  24. TRM.42750 Storage Unit Alarms

    An excellent question. In theory, there is no such thing as "too cold" for a freezer, so the low temp. alarm setting seems to be pointless. However, if such a unit does activate a low temp. alarm, it may indicate that the unit is malfunctioning in some way. It might just give you time to intervene before the unit goes "bang". I hope I've sufficiently emphasized the low probabilities of the above happening. Our facility still checks the low alarm points for our walk-in freezers (-20 C). Luckily, we have access to liquid nitrogen (LN2) which is very convenient and quick. In the past, we've very awkwardly used a sludge of alcohol and dry-ice to get a very low temperature (-60 C), but this doesn't help with ultra-colds (-80 C). For physical science reasons, we are unable to activate the low alarm on our liquid nitrogen tank !!!! We actually had an inspector challenge us on this issue a number of years ago.
  25. Lewis A

    Given the number of reactions that are recorded in the literature, which are very few, and the fact that the small amount of plasma left on the red cells to be transfused will very readily inhibit in vivo the anti-Lea in the patients plasma, I think this is overkill.
  26. Eluates on babies with positive DATs

    How do you know that the positive DAT is due to ABO incompatibility, unless an elution is performed. Surely, you are making an assumption (a very likely assumption, but an assumption nevertheless)?
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