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PathLabTalk would like to wish all members celebrating their birthday today a happy birthday. andean (64)jkling (70)mmccormick (70)bloodrek (56)Dolores (63)acarhakan (62)caldwellrcrj (56)awni (52)omartally (54)Marina Setaro (25)shawnsimpson834 (34)

I guess the solid phase is more sensitive to detect the auto in this patient. I will use the same method as detecting the antobodies to do crossmatch. Of course I will do saline and AHG crossmatch as well. Just my humble opinion.

We use Lumena (solid phase) for the ID. Crossmatch is tube method IS, LISS 37C, IgG

I am kind of curious about is the crossmatches and ABID using the same method?

At first I thought it might have been a high frequency antigen but it didn’t make sense as my crossmatches were compatible. I also thought it might be a cold antibody that reacts on IgG? I’m not very knowledgable on how cold antibody works and can’t back up how I came up with that 😅

Patient is identified as having “warmautoantibodies” in our lab with transfusion instruction to give C, E, K neg units. Patient was phentotyped for RH and Kell on the first work-up. When patient came back for a type and screen, 2 cell screen were 2+, ABID were all 1+ except for one cell being 3+. DAT was negative and when I performed tube screen and autocontrol, they’re both negative. When I looked at the previous work-up, no transfusion has been done since 2018, screen and ABID also have panagglutination reactions. DAT and Tube screen were also negative. My supervisor asked me to do an elution and it came out with panagglutination reaction (3+ and 4+). Since patient also needed blood, I IgG crossmatched 4 units of C, E, K neg and they were all compatible. Our facility called it as warm autoantibody but it just didn’t sit well with me. Should I have done further testing to rule out if that one 3+ meant something else on the ID? Thank you!

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"Nice crisp photographs, by the way." thanks thanks for advice

Are you looking for rally difficult cases, or more commonplace cases?

It looks like an A subgroup B to me. The apparent reaction with the A cells and the donor's plasma in the reverse group in the first photograph could be a weak anti-A1, but more likely it is another specificity altogether (such as an anti-P1). Without a doubt, however, a sample from the donor should be sent to a Reference Laboratory to have the ABO type sorted out (e.g. by adsorption and elution tests, preferably using a strong human-derived polyclonal anti-A). Nice crisp photographs, by the way.

Does anyone know of a book I could buy to help me practice case studies and get a more in depth understanding of Transfusion situations?

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this is a donor blood grouping also with tube tech anti A given Neg reaction !!! what could be the reason?

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Happy Birthday!

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Hats off to Nicole and the whole IBGRL team