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Arno last won the day on March 17

Arno had the most liked content!

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    Scientist with more than 20 years of experiences in blood banks, transfusion centers, hospitals and national health authority. I believe we share a common passion for Immunohematology and for the moment I do work for a private company in Switzerland (involved in marketing, education, biological support).

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  1. Hi! I do not know which gel card supplier you are using, but the one I “know” use 50ul of A1 and B cells with 25ul of plasma and an incubation at 37°C for 15 minutes. And all of this in an AHG gel card of course… having previously checked as well there is no additional antibody that could interfere (result from mother if available/antibody screening result). Hope it helps.
  2. I just answered this question. My Score PASS  
  3. I hereby forward you the link to the registration page => https://info.bio-rad.com/ww-IHD-transfusion-w-registration-lp2.html?WT.mc_id=201015029401 You'll see that if cannot make it for the live session (due to time difference), the recording will be made available about 1 hour after the live session using the same link that will be sent to you by email and you will have the opportunity to watch it at any time (and an unlimited number of times...).
  4. In this context (post transfusion with DAT pos, screening neg), would be worth running/trying an elution?
  5. Should be HbS negative as decreasing the proportion of HbS (compared to HbA) prevents complications/crisis of SCD related to vaso-occlusion. So not only for crisis but to prevent the crisis. https://onlinelibrary.wiley.com/doi/full/10.1111/bjh.14346
  6. Thank you for your feedback - much appreciated. This confirms my understanding and what I have read so far on this topic. Thanks again.
  7. Hi there! I hope you are all doing well. The Italian blood transfusion society has made the screening of Covid-19 patient for IgA deficiency systematic before the transfusion of convalescent plasma. http://isbtweb.org/fileadmin/user_upload/Italy.pdf Would like to know what is your position in this regards and is there any similar existing guidelines? Thank you in advance
  8. Yes indeed different pH, different suppliers may explain such a behavior (some anti-M are enhanced with acidification of plasma). In addition, Anti-M often shows dosage effect but I believe you have antigen M double dose cells on your panel too. What are the phenotype of the 2 cells reacting in screening and the one not reacting? Is your patient antigen M negative? It also exists the anti-M1 (the M1 antigen belongs to the MN CHO collection) that reacts with some M positive cells and stronger with M/N positive cells (M1 is expressed on M positive cells) and it can be, though
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