Hi Clarest,
I am not familiar with the MTS product line, but to me it says that enzyme treated cells can be used in AHG card (the human anti-IgG is already in the gel card of course) though you may experience some non specific reactions plus a higher detection rate of unclinically significant antibodies (specific but unwanted...). This uses (enzyme + AHG), as mentionned above, should be well understood/assessed/validated/targeted => you will as well enhance the reactivities for other clinically significant antibodies such as anti-Jka which is the purpose. Then, they rather recommend to use these enzyme treated cells in their "neutral-saline" cards (called buffered gel), which does not contain AHG. It is propably a way to prevent you to complain if you get an increase of positive reactions to be further investigated whereas this is mostly due to unclinically significant antibodies. Enzyme reactive antibodies (e.g. anti-Rh) are "expected" (why antibodies do no read IH books...) to agglutinate in neutral-saline cards as ABO agglutinins do, without AHG. Having said that, anti-Jka are better detected in coombs + enzyme ....