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SMILLER last won the day on October 20

SMILLER had the most liked content!



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    Has been around for a while
  • Birthday 08/10/1958

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  • Biography
    Medical Laboratory Scientist
  • Location
    Saginaw, MI, USA
  • Occupation
    Generalist, mid-sized level 2 trauma center
  1. Post Vasectomy Sperm Presence

    Did you try a hemocytometer? It seems like a count of less than 100 / ul would be doable on one. See: http://www.vivo.colostate.edu/hbooks/pathphys/reprod/semeneval/hemacytometer.html (you can find anything on the internet!) Scott
  2. Specimen Collection and Processing

    In general, our procedures require a specimen up to 48 hours old, as long as it is stored refrigerated. Usually specimen testing is begun soon after the specimen reaches the blood bank, where they are left at room temp while that is completed. Scott
  3. Lewis A

    From you to our pathologist's ear, Malcolm! Scott
  4. Antigen Tested Units

    Right. But please look at AABB standard 5.8.4. As I mentioned above, the donor processing facility can only label a unit as tested negative (far a particular antigen) if that has been done on two separate occasions. In that case, or if the unit in question was tested by the donor facility, the donor facility could indeed label the unit as "negative for RBC antigens". Otherwise, it would have to be tested, either by the donor facility or when it reaches your blood bank. After all, every donation by a donor is typed twice for ABO before a patient gets it. Scott
  5. wAIHA with IgM and C3c/C3d coating

    Vel, as always, I appreciate your response, Malcolm. Scott
  6. Lewis A

    Ja. Here, if the anti-Le a shows up on an inpatient, we set up 2 AHG crossmatched units, because that is what we do for all atypical antibody patients. But if it does not show up in the antibody screen, we have to order confirmed Le a units from our blood supplier and XM those! It's so 20th century... Scott
  7. wAIHA with IgM and C3c/C3d coating

    Isn't it possible, for a new antibody, to have both IgM and undetectable IgG produced at the same time, or even IgM without IgG, at least at first conversion? Scott
  8. CAP TRM.41350

    I would say that since they mention "a label or tag", that they are thinking that a label would be something that is stuck to the unit, and between the labeled unit and the transfusion tag all that required info would be there. Scott
  9. Lewis A

    Are we the only ones still screening units for patients producing anti-Lewis A? Is there ever a reason to do this for routine cross matches? Scott
  10. Antigen Tested Units

    Right. They also have to include things like expiration date. product code, etc. So between the tag from us and they label already on the unit, I guess it is all covered. Scott
  11. CAP TRM.41350

    Interesting standards from CAP. AABB only requires the unit to be tagged by the transfusion service with two recipient IDs, donor unit ID number, and compatibility info. At the bedside, ABO/Rh must be checked between patient and donor unit. We have the patient ABO/Rh on the tag. Scott
  12. Antigen Tested Units

    The above is interesting, and it is found under Transfusion Services standards. Under collection facility standards, 5.8.4 says that untested units for "other" antigens may be labeled negative only if units from two previous donations were tested by the collection facility. Does anyone get this kind of information from their blood supplier? We just either get a "confirmed negative" or a "historical negative" label, with the historical label specifically stating that confirmatory testing must be done. So I guess we are stuck with that. Not really much of an issue here though! Scott
  13. Antigen Tested Units

    There are two types of "antigen negative" units we can get from our supplier here in Michigan. One is "historically negative" -- those have to be retested when they arrive. The other type is "confirmed" -- those units have been confirmed negative for a particular antigen at the supplier and do not need to be retested here. Scott
  14. For cell counts on various fluids, we simply use a hemacytometer without any staining. I would think that it is pretty common? Scott
  15. Daily Reagent QC requirements

    Really, then you don't run an auto-control with new AB Pos patients becayse A, B and O cover "everything". Its going to come down to what your regulator pulls up regarding whatever standards they use: FDA, CAP, etc. Granted, some of them do not make sense when you think of "science" stuff like serology and phyisiology, but you have to pass those inspections! Has anyone ever talked an inspector out of something that they have a standard for? Scott