Posted April 9, 20196 yr comment_76433 Let's try this again. Anyone using negative controls for their backtype reagents (A1 cell and B cells)?? If so, what are you using?
April 9, 20196 yr comment_76440 I'm pretty sure any of the commercial QC systems will have this covered and I don't remember them being all that expensive. I have used both the Ortho and Immucor systems and don't remember any issues.
April 10, 20196 yr comment_76448 16 hours ago, Malcolm Needs said: Anti-A for the group B red cells and anti-B for the A1 red cells. Same or QC material from Quotient or BioRad works
April 11, 20196 yr comment_76473 We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive.
April 12, 20196 yr comment_76492 I also use Anti-B for affirmagen A1 rgt neg ctl and Anti-A for affirmagen B rgt neg control as part of new lot/newshipment check. as part of daily control I use ortho confidence
April 15, 20196 yr comment_76517 Plasma from an AB patient (negative antibody screen, no issues w/ blood type).
April 15, 20196 yr comment_76522 On 4/11/2019 at 9:09 AM, NicolePCanada said: We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive. We do the same.
April 19, 20196 yr comment_76556 If I am qcing gel - I use the diluent as a neg control. If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc. (personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA).
April 20, 20196 yr comment_76562 22 hours ago, David Saikin said: If I am qcing gel - I use the diluent as a neg control. If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc. (personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA). Hmmm. Here in Michigan, we are indeed doing negative controls for reverse cells (we just use albumin). We are FDA and JCAHO inspected. Scott
April 20, 20196 yr comment_76564 On 4/9/2019 at 2:16 AM, Malcolm Needs said: Anti-A for the group B red cells and anti-B for the A1 red cells. By definition, reagent reverse grouping A1 and B cells are used to detect anti-A and anti-B antibody in patient plasma. Accordingly A1 cells should react with anti-A but not with anti-B, and B cells should react with anti-B but react with anti-A. Therefore, A1 cells should not be agglutinated by anti-B. No agglutination is a negative test result, i.e., a negative control test. Likewise, B cells should not be agglutinated by anti-A. No agglutination is a negative test result, i.e. a negative control test. Testing A1and B cells with AB plasma, Diluent, Albumin or saline may demonstrate that the test cells are not spontaneously agglutinating in their presence which serves as a negative control for those reagents, but does not serve as a negative control test for A1 cells or B cells.
April 24, 20196 yr comment_76591 We use Ortho Confidence antisera for the positive QC. Anti-A for the group B red cells and anti-B for the A1 red cells for the negative QC.
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