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comment_76433

Let's try this again. Anyone using negative controls for their backtype reagents (A1 cell and B cells)?? If so, what are you using? 

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  • Malcolm Needs
    Malcolm Needs

    Anti-A for the group B red cells and anti-B for the A1 red cells.

  • ORTHO CONFIDENCE

  • John C. Staley
    John C. Staley

    I'm pretty sure any of the commercial QC systems will have this covered and I don't remember them being all that expensive.  I have used both the Ortho and Immucor systems and don't remember any issue

comment_76434

Anti-A for the group B red cells and anti-B for the A1 red cells.

comment_76440

I'm pretty sure any of the commercial QC systems will have this covered and I don't remember them being all that expensive.  I have used both the Ortho and Immucor systems and don't remember any issues.  

comment_76448
16 hours ago, Malcolm Needs said:

Anti-A for the group B red cells and anti-B for the A1 red cells.

Same or QC material from Quotient or BioRad works

comment_76473

We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive.

comment_76492

I also use Anti-B for affirmagen  A1 rgt  neg ctl  and Anti-A for affirmagen B rgt  neg control as part of new lot/newshipment check. as part of daily control I use ortho confidence

comment_76517

Plasma from an AB patient (negative antibody screen, no issues w/ blood type).

comment_76522
On 4/11/2019 at 9:09 AM, NicolePCanada said:

We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive.

We do the same.

comment_76556

If I am qcing gel - I use the diluent as a neg control.

If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc.

(personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA).

comment_76562
22 hours ago, David Saikin said:

If I am qcing gel - I use the diluent as a neg control.

If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc.

(personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA).

Hmmm.  Here in Michigan, we are indeed doing negative controls for reverse cells (we just use albumin).  We are FDA and JCAHO inspected.

Scott

comment_76564
On 4/9/2019 at 2:16 AM, Malcolm Needs said:

Anti-A for the group B red cells and anti-B for the A1 red cells.

By definition, reagent reverse grouping A1 and B cells are used to detect anti-A and anti-B antibody in patient plasma.  Accordingly A1 cells should react with anti-A but not with anti-B, and B cells should react with anti-B but react with anti-A.  Therefore, A1 cells should not be agglutinated by anti-B.  No agglutination is a negative test result, i.e., a negative control test.  Likewise, B cells should not be agglutinated by anti-A. No agglutination is a negative test result, i.e. a negative control test.

Testing A1and B cells with AB plasma, Diluent, Albumin or saline may demonstrate that the test cells are not spontaneously agglutinating in their presence which serves as a negative control for those reagents, but does not serve as a negative control test for A1 cells or B cells.

comment_76591

We use Ortho Confidence antisera for the positive QC. Anti-A for the group B red cells and anti-B for the A1 red cells for the negative QC.

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