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Let's try this again. Anyone using negative controls for their backtype reagents (A1 cell and B cells)?? If so, what are you using? 

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We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive.

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I also use Anti-B for affirmagen  A1 rgt  neg ctl  and Anti-A for affirmagen B rgt  neg control as part of new lot/newshipment check. as part of daily control I use ortho confidence

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On 4/11/2019 at 9:09 AM, NicolePCanada said:

We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive.

We do the same.

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If I am qcing gel - I use the diluent as a neg control.

If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc.

(personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA).

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22 hours ago, David Saikin said:

If I am qcing gel - I use the diluent as a neg control.

If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc.

(personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA).

Hmmm.  Here in Michigan, we are indeed doing negative controls for reverse cells (we just use albumin).  We are FDA and JCAHO inspected.

Scott

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On 4/9/2019 at 2:16 AM, Malcolm Needs said:

Anti-A for the group B red cells and anti-B for the A1 red cells.

By definition, reagent reverse grouping A1 and B cells are used to detect anti-A and anti-B antibody in patient plasma.  Accordingly A1 cells should react with anti-A but not with anti-B, and B cells should react with anti-B but react with anti-A.  Therefore, A1 cells should not be agglutinated by anti-B.  No agglutination is a negative test result, i.e., a negative control test.  Likewise, B cells should not be agglutinated by anti-A. No agglutination is a negative test result, i.e. a negative control test.

Testing A1and B cells with AB plasma, Diluent, Albumin or saline may demonstrate that the test cells are not spontaneously agglutinating in their presence which serves as a negative control for those reagents, but does not serve as a negative control test for A1 cells or B cells.

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