Daily QC (again)

[jojo808 35]

Let's try this again. Anyone using negative controls for their backtype reagents (A1 cell and B cells)?? If so, what are you using? 

[Malcolm Needs ★ 4,230]

Anti-A for the group B red cells and anti-B for the A₁ red cells.

[ANORRIS 80]

ORTHO CONFIDENCE

[John C. Staley 983]

I'm pretty sure any of the commercial QC systems will have this covered and I don't remember them being all that expensive.  I have used both the Ortho and Immucor systems and don't remember any issues.  

[Kathyang 37]

  We use saline just to make sure they aren't just agglutinating.

[pinktoptube 44]

16 hours ago, Malcolm Needs said:

Anti-A for the group B red cells and anti-B for the A₁ red cells.

Same or QC material from Quotient or BioRad works

[NicolePCanada 14]

We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive.

[Karen knight 2]

I also use Anti-B for affirmagen  A1 rgt  neg ctl  and Anti-A for affirmagen B rgt  neg control as part of new lot/newshipment check. as part of daily control I use ortho confidence

[Bb_in_the_rain 76]

Ortho Confidence

[SMILLER 739]

Albumin.

Scott

[AMcCord 1,121]

Plasma from an AB patient (negative antibody screen, no issues w/ blood type).

[BankerGirl 170]

On 4/11/2019 at 9:09 AM, NicolePCanada said:

We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive.

We do the same.

[David Saikin 1,202]

If I am qcing gel - I use the diluent as a neg control.

If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc.

(personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA).

[SMILLER 739]

22 hours ago, David Saikin said:

If I am qcing gel - I use the diluent as a neg control.

If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc.

(personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA).

Hmmm.  Here in Michigan, we are indeed doing negative controls for reverse cells (we just use albumin).  We are FDA and JCAHO inspected.

Scott

[Dansket 371]

On 4/9/2019 at 2:16 AM, Malcolm Needs said:

Anti-A for the group B red cells and anti-B for the A₁ red cells.

By definition, reagent reverse grouping A₁ and B cells are used to detect anti-A and anti-B antibody in patient plasma.  Accordingly A₁ cells should react with anti-A but not with anti-B, and B cells should react with anti-B but react with anti-A.  Therefore, A₁ cells should not be agglutinated by anti-B.  No agglutination is a negative test result, i.e., a negative control test.  Likewise, B cells should not be agglutinated by anti-A. No agglutination is a negative test result, i.e. a negative control test.

Testing A₁and B cells with AB plasma, Diluent, Albumin or saline may demonstrate that the test cells are not spontaneously agglutinating in their presence which serves as a negative control for those reagents, but does not serve as a negative control test for A₁ cells or B cells.