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jojo808

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jojo808 last won the day on January 24 2016

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  1. Forgive my ignorance but what is the positive reaction you get when testing reagent Anti-A with A2 cells due to??
  2. There is an article from George Garretty called Problems Associated With Passively Transfused Blood Group Alloantibodies that kind of mentions this. Although I feel it is perfectly safe to give out of group platelets, (we have done so for years) my concern was at what point would it interfere, if ever, with ABO/Rh type testing with tube method? According to the article worst case would be positive DAT but again I wonder if it would be detected in the plasma
  3. Hope someone can clear things up for me: 1. Can a type B recipient have 'testable' anti-B, acquired passively via transfusion of a few type A and type O platelets?? Let's say one out of type per day for a week. 2. Does Type B and Type O persons have naturally occurring Anti-A2?? Inquiring minds want to know, thanks in advance.
  4. Previous Quote from Malcom Needs while searching older content on this subject: "In most cases, TRALI is caused by donor leucocyte antibodies reacting with alloantigens present on the patient's leucocytes, although patient alloantibodies have been involved in some rare cases. The antibodies concerned are usually HLA class I and II specific, but HNA antibodies have also caused this". The thread was actually discussing solvent detergent plasma. When investigating possible TRALI, exactly what tests are usually ordered on the implicated donor? (Antibodies against HLA class I and II? And what are HNA antibodies and what's the difference between HLA and HNA antibodies? Aren't they both testing for antibodies against white cells?? Also if donor is found to have HLA antibodies, does it matter to identify them and then test the recipient for the antigen??? I'm a little confused.
  5. Just wondering why most use a 2nd sample only within 24 hours of collection. Does anyone see anything wrong with testing an older sample, say 3-4 days old??
  6. Does anyone audit the units transfused in the OR? How does the individual units transfused in the OR go into the patients record? Does the units go on a flow-sheet and someone transcribes this into the chart? Does the whole flow-sheet get scanned somewhere in the chart? I'm just trying to get as much information prior to a meeting about this. It seems like most are using coolers in the OR which seems 'more safe' than one refrigerator to 'share'. I'm willing to trust the process once it leaves the blood bank as long as that is compliant with AABB and CAP.
  7. I do almost exactly like AmcCord except I do not discard thermometers every year (but it's sounding really nice right now). I also have a spreadsheet with the serial numbers listed and the location (refrigerator/ freezer/ room temp .....) there is a column on the spreadsheet to answer if the calibration was acceptable or not. If the answer is no (>1C from the NIST) then a comment of "discarded" is added to that row. Our old SOP had instructions for correction factors that meant if the thermometer is 0.5 C higher (warmer) than the certified, a label must be affixed to the thermometer that lists the correction factor (- 0.5 C correction factor) which means you minus 0.5 C from that thermometer reading. I wouldn't waste my time with correction factors, all techs will not remember to use it, just discard it and purchase new ones, less headache for you.
  8. Thank you everyone for your responses. As wrong as it sounds, it gives me some peace knowing that there are others going through or have gone through the same battles. And like John stated there are others 'stepping in' and just complicating the situation for blood bank with solutions that make matters worse, not better. I really appreciate the pep talk and I will pick my battles carefully and continue to do the best that we possibly can for the sake of our patients that we serve. I feel better now
  9. I have read several threads, some maybe 10 years ago regarding this matter but I didn't see anyone really addressing the following. My question is does anyone work at a hospital where anesthesia scans in the blood unit prior to transfusion?? According to AABB 5.28.4 "The transfusionist and one other individual (or an electronic identification system) shall, in the presence of the recipient, positively identify the recipient and match the blood component to the recipient through the use of 2 independent identifiers". There is also a similar statement from CAP TRM.41300. We had a near miss several years ago, same situation, different place. One refrigerator being shared in the OR, 2 big cases going on, you get the scenario. To me, it doesn't matter how great the cooler, refrigerator, blood tracking .... there is no fool proof system but can we get close to one? one of the threads addressed the Joint Commissions Sentinel Event Alert regarding blood for multiple OR patients in the same refrigerator among other things (1999). This was 20 years ago!!!! Have we not improved this in 20 years???? Is it that hard to scan in a blood unit? Does it not take more than 5 seconds to do this??? The people making these computer decisions at our facility just can't see how important this is. Geez and in this day and age of computers all I get is "Our computer system cannot currently check this and that and blah blah blah is all I hear. Calgon take me away! Sorry for the rant but I needed to get that off my chest.
  10. I totally agree with David. I would say most of our Anti-M's react only with the cells that have the homozygous expression and not with the heterozygous expression. I would say you have to call it an Anti-M especially if Gel is your primary method of testing with tube as your backup. I don't think you can call it negative just because the tube has no reactivity. We sometimes (very rarely) would have a Warm autoantibody that showed pan-agglutination in Gel but was negative with tube method at one hour incubation, no enhancement excluding the auto control which is usually still reactive. We would just add a comment about the negative results in tube method just for proper documentation but still result as a Warm autoantibody.
  11. Let's try this again. Anyone using negative controls for their backtype reagents (A1 cell and B cells)?? If so, what are you using?
  12. Hi all, I am trying to overhaul our policy for transfusion reactions as recommended by our last CAP inspector. We were basically culturing all reactions except urticaria and the inspector said we were wasting time and energy and I agree but I need more assurance for the definite criteria so I want to know what are your criteria for culture and gram stain for blood products that are in question. I've looked online and found some use only temperature increase while others use temp increase with or without other symptoms What my research has found is the following: Most call febrile reactions bet 38-40C, or an increase in temp bet 1-2C from the baseline (pre-transfusion). The ones that use other symptoms use tachycardia and/or hypotension. Just want a poll for several things: 1. At what temp do you call it a Febrile reaction? 2. Do you also use temp increase from the baseline? If so what is? 3. Do you use other criteria with Temp increase for culture? If so what are they? 4. If you do have one, what is your definition for tachycardia (eg =>100 bpm?) 5. Same with Hypotension, any numerical definition? I know there are threads on this but I don't want to sort through it all. Please, I would love as much input as possible. Add any advice or other pertinent information is greatly appreciated. Thank you all in advance.
  13. We have had a couple cases like tkakin where the 'cold' shows in the tube but because the Gel is incubated at 37 it is spun to a negative screen. Not sure what a settle test is but what usually works for us is warming the patient's plasma well at 37C prior to testing with a1 cell and b cell. Sounds like a pesky cold agglutinin if warming corrects your MCHC.
  14. Most references do not use the single symptom of increased BP to indicate a TACO reaction. They usually list a variety of symptoms in which a combination of 3 or more of the following need to be present: Increased BP, Respiratory distress (dyspnea), Acute or worsening pulmonary edema via xray, tachycardia, jugular vein distension, increased BNP (brain natriuretic peptide), response to diuretics...... It's up to your facility to decide what kind of criteria you want in your policy. In any case I would think this is important to note in the patient's file since the patient receives dialysis and would probably need to be transfused sitting upright and very slowly if this is indeed a TACO or TACO-like reaction for future transfusions.
  15. Thank you, ALL the responses were helpful to me!
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