Posted March 18, 201312 yr comment_50036 Does anyone use expired ABID Panels to help rule out cells homozygously?
March 18, 201312 yr Author comment_50039 What kind of control regimen do you use? Our sister hospital just got cited from CAP for not performing QC on the expired panel of use and are not sure what to use for QC.
March 19, 201312 yr comment_50043 We use known, very weak, antibodies of the same specificity as we are trying to rule out. For example, if we are trying to rule out a weak anti-K, which only reacts with presumed homozygous K expression, we use a known, very weak anti-K to ensure that the K antigen on the expired ABID red cells has not degraded.
March 19, 201312 yr comment_50051 Malcolm - are these the weak abs you spoke about earlier? . . . provided by your nat'l service.We do not have that luxury in the USA.I use a 1:4 dilution of either Fya or Fyb: have not had to deal with Fya-b- cell yet.
March 19, 201312 yr comment_50055 Well, some are David (anti-D, anti-c, anti-K and anti-Fya), but others are just some that we have collected from patients over the years and frozen down for just such an eventuality.
March 19, 201312 yr comment_50057 We keep panel cells for 4 months. Our SOP states they are acceptable until they start to hemolyze or turn brown. We have never been cited by AABB for using them.
March 19, 201312 yr comment_50060 We keep our panels until they start to hemolyze. We do run QC on all our expired panels. We use Anti-Fya typing sera with a heterozygous positive Fya cell and a Fka negative cell. we have never been cited by CAP for this.
March 19, 201312 yr comment_50062 Blow me! 4 months seems a bit long to me, even in CellStab!That made me burst out laughing. In America that would be a very vulgar statement indeed !
March 19, 201312 yr comment_50064 Here is an ongoing question regarding panel cell QC. If you QC your expired panels cells, do you QC your in date panel cells? For those of you that use Anti-Fy, what if the antibody you are trying to ID is not a Fy? To be truly accurate with QC, would you not need to test every cell for every antigen listed? Haven't we all found examples of antibodies that haven't read the book and do not react with all the cells they are supposed to? Is it because of the antibody or because of the cell? Would QC help in this situation?Just playing devil's advocate
March 19, 201312 yr comment_50068 That made me burst out laughing. In America that would be a very vulgar statement indeed !Oooops. Sorry. What is it? Friends divided by a common language - or something along those lines!!!!!!!!!!!!!!!!!!
March 19, 201312 yr comment_50074 Does anyone use expired ABID Panels to help rule out cells homozygously?We use commercial antisera as positive control and saline solution as negative control.
March 20, 201312 yr comment_50084 We do not use expired cells to rule out, only sometimes to prove the specificity (extra positive).But when you do it you have to do the controls that Malcolm sugested, show that there is weakening of the antigen you use to rule out. In the absence of a weak antibody you can switch to a diluted antibody or perform the antigen expression in titer compaired to a cell that is not expired. (a weak antibody is better, but this can be used as an alternative).The "4 months rule" did not make me say the same as Malcolm but I needed a few minutes to get from the flour on my chair again.Peter
April 10, 201312 yr comment_50360 Malcolm, I have tried to come up with a way to explain the phrase, but words that will not get me in trouble elude me.
April 10, 201312 yr comment_50361 Which phrase Elizabeth? - send me a private message if it is that embarrassing (or email me on Malcolm.needs@blueyonder.co.uk).
April 11, 201312 yr comment_50376 Which phrase Elizabeth? - send me a private message if it is that embarrassing (or email me on Malcolm.needs@blueyonder.co.uk).With respect to 'Blow me'; this would be the same as 'That's a funny thing' or 'Blow me down', generally 'What a surprise!'
April 12, 201312 yr comment_50418 Oooops. Sorry. What is it? Friends divided by a common language - or something along those lines!!!!!!!!!!!!!!!!!!Malcolm, in the U.S. it's a euphemism for oral sex.---We QC our expired panel cells with the corresponding commercial antisera to whatever antigen we are using that particular cell to rule out with. (Example: If using an expired K+ cell to rule out Anti-K, we test the cell with commerical Anti-K)
April 13, 201312 yr comment_50424 Om my goodness. I'm sorry if I have caused any offence. It doesn't mean that over here.
April 15, 201312 yr comment_50431 I'm sure no one is offended. We are all developing a greater global awareness, right? Last I knew no one in the UK would use the nickname Randy for someone named Randall, but that is common in the US. Edited April 15, 201312 yr by Mabel Adams
April 15, 201312 yr comment_50433 I hope my institute will not shut down the connection to this filty forum.
April 17, 201312 yr comment_50528 Another QC Method I have used is: On the outdated panel, run a cell that is positive (and reactive) for the "known" antibody and look for a similar strength of reaction. For example, let's say the patient has Anti-Jka reacting 2+ (indated panel), Anti-C reacting 1+ (indated panel), and you are using your outdated panel to rule-out Anti-S (so would need to run a cell that is Jka-, C-, S+)....you would also test a cell that is Jka+ or C+ and see if the strength of reactivity on the outdated panel is comparable to the strength of reactivity on the indated panel. That would be your control cell.Just a thought...Brenda Hutson, CLS(ASCP)SBB
April 17, 201312 yr comment_50535 Just because the Jka or C antigens were up to snuff, does that mean the S antigen would still be detectable?
April 18, 201312 yr comment_50544 We don't know that for sure on our in-date panels either. Someone may have spilled bleach nearby and destroyed the S antigen in some vials. The only way to be totally sure would be to QC every antigen on every cell every day of use.
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