Kristina Posted March 18, 2013 Share Posted March 18, 2013 Does anyone use expired ABID Panels to help rule out cells homozygously? Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted March 18, 2013 Share Posted March 18, 2013 Yes, but with a very strict control regimen. Link to comment Share on other sites More sharing options...
Kristina Posted March 18, 2013 Author Share Posted March 18, 2013 What kind of control regimen do you use? Our sister hospital just got cited from CAP for not performing QC on the expired panel of use and are not sure what to use for QC. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted March 19, 2013 Share Posted March 19, 2013 We use known, very weak, antibodies of the same specificity as we are trying to rule out. For example, if we are trying to rule out a weak anti-K, which only reacts with presumed homozygous K expression, we use a known, very weak anti-K to ensure that the K antigen on the expired ABID red cells has not degraded. OxyApos 1 Link to comment Share on other sites More sharing options...
David Saikin Posted March 19, 2013 Share Posted March 19, 2013 Malcolm - are these the weak abs you spoke about earlier? . . . provided by your nat'l service.We do not have that luxury in the USA.I use a 1:4 dilution of either Fya or Fyb: have not had to deal with Fya-b- cell yet. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted March 19, 2013 Share Posted March 19, 2013 Well, some are David (anti-D, anti-c, anti-K and anti-Fya), but others are just some that we have collected from patients over the years and frozen down for just such an eventuality. Link to comment Share on other sites More sharing options...
pbaker Posted March 19, 2013 Share Posted March 19, 2013 We keep panel cells for 4 months. Our SOP states they are acceptable until they start to hemolyze or turn brown. We have never been cited by AABB for using them. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted March 19, 2013 Share Posted March 19, 2013 Blow me! 4 months seems a bit long to me, even in CellStab! Link to comment Share on other sites More sharing options...
Kathyang Posted March 19, 2013 Share Posted March 19, 2013 We keep our panels until they start to hemolyze. We do run QC on all our expired panels. We use Anti-Fya typing sera with a heterozygous positive Fya cell and a Fka negative cell. we have never been cited by CAP for this. Link to comment Share on other sites More sharing options...
goodchild Posted March 19, 2013 Share Posted March 19, 2013 Blow me! 4 months seems a bit long to me, even in CellStab!That made me burst out laughing. In America that would be a very vulgar statement indeed ! Jyoung and Gnapplec 2 Link to comment Share on other sites More sharing options...
pbaker Posted March 19, 2013 Share Posted March 19, 2013 Here is an ongoing question regarding panel cell QC. If you QC your expired panels cells, do you QC your in date panel cells? For those of you that use Anti-Fy, what if the antibody you are trying to ID is not a Fy? To be truly accurate with QC, would you not need to test every cell for every antigen listed? Haven't we all found examples of antibodies that haven't read the book and do not react with all the cells they are supposed to? Is it because of the antibody or because of the cell? Would QC help in this situation?Just playing devil's advocate Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted March 19, 2013 Share Posted March 19, 2013 That made me burst out laughing. In America that would be a very vulgar statement indeed !Oooops. Sorry. What is it? Friends divided by a common language - or something along those lines!!!!!!!!!!!!!!!!!! Link to comment Share on other sites More sharing options...
BBCLS Posted March 19, 2013 Share Posted March 19, 2013 Does anyone use expired ABID Panels to help rule out cells homozygously?We use commercial antisera as positive control and saline solution as negative control. Link to comment Share on other sites More sharing options...
SMILLER Posted March 20, 2013 Share Posted March 20, 2013 "Well blow me down!" Link to comment Share on other sites More sharing options...
Rh-fan Posted March 20, 2013 Share Posted March 20, 2013 We do not use expired cells to rule out, only sometimes to prove the specificity (extra positive).But when you do it you have to do the controls that Malcolm sugested, show that there is weakening of the antigen you use to rule out. In the absence of a weak antibody you can switch to a diluted antibody or perform the antigen expression in titer compaired to a cell that is not expired. (a weak antibody is better, but this can be used as an alternative).The "4 months rule" did not make me say the same as Malcolm but I needed a few minutes to get from the flour on my chair again.Peter Link to comment Share on other sites More sharing options...
EDibble Posted April 10, 2013 Share Posted April 10, 2013 Malcolm, I have tried to come up with a way to explain the phrase, but words that will not get me in trouble elude me. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted April 10, 2013 Share Posted April 10, 2013 Which phrase Elizabeth? - send me a private message if it is that embarrassing (or email me on Malcolm.needs@blueyonder.co.uk). Link to comment Share on other sites More sharing options...
John Eggington Posted April 11, 2013 Share Posted April 11, 2013 Which phrase Elizabeth? - send me a private message if it is that embarrassing (or email me on Malcolm.needs@blueyonder.co.uk).With respect to 'Blow me'; this would be the same as 'That's a funny thing' or 'Blow me down', generally 'What a surprise!' Link to comment Share on other sites More sharing options...
jgabbard Posted April 12, 2013 Share Posted April 12, 2013 Oooops. Sorry. What is it? Friends divided by a common language - or something along those lines!!!!!!!!!!!!!!!!!!Malcolm, in the U.S. it's a euphemism for oral sex.---We QC our expired panel cells with the corresponding commercial antisera to whatever antigen we are using that particular cell to rule out with. (Example: If using an expired K+ cell to rule out Anti-K, we test the cell with commerical Anti-K) Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted April 13, 2013 Share Posted April 13, 2013 Om my goodness. I'm sorry if I have caused any offence. It doesn't mean that over here. Link to comment Share on other sites More sharing options...
Mabel Adams Posted April 15, 2013 Share Posted April 15, 2013 (edited) I'm sure no one is offended. We are all developing a greater global awareness, right? Last I knew no one in the UK would use the nickname Randy for someone named Randall, but that is common in the US. Edited April 15, 2013 by Mabel Adams Link to comment Share on other sites More sharing options...
Rh-fan Posted April 15, 2013 Share Posted April 15, 2013 I hope my institute will not shut down the connection to this filty forum. Link to comment Share on other sites More sharing options...
Brenda K Hutson Posted April 17, 2013 Share Posted April 17, 2013 Another QC Method I have used is: On the outdated panel, run a cell that is positive (and reactive) for the "known" antibody and look for a similar strength of reaction. For example, let's say the patient has Anti-Jka reacting 2+ (indated panel), Anti-C reacting 1+ (indated panel), and you are using your outdated panel to rule-out Anti-S (so would need to run a cell that is Jka-, C-, S+)....you would also test a cell that is Jka+ or C+ and see if the strength of reactivity on the outdated panel is comparable to the strength of reactivity on the indated panel. That would be your control cell.Just a thought...Brenda Hutson, CLS(ASCP)SBB Link to comment Share on other sites More sharing options...
goodchild Posted April 17, 2013 Share Posted April 17, 2013 Just because the Jka or C antigens were up to snuff, does that mean the S antigen would still be detectable? Link to comment Share on other sites More sharing options...
Mabel Adams Posted April 18, 2013 Share Posted April 18, 2013 We don't know that for sure on our in-date panels either. Someone may have spilled bleach nearby and destroyed the S antigen in some vials. The only way to be totally sure would be to QC every antigen on every cell every day of use. Link to comment Share on other sites More sharing options...
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