Great questions. We have had the ECHO for many years now, and see this phenomenon fairly often. We have a flowchart to determine the steps to take. This is the short version: If the patient reacts on the ECHO in all screening cells, and they have no antibody history, we do an ECHO panel. There could be an antibody to a high frequency antigen, or a mixture of multiple antibodies causing all screening cells to react. If the panel is also all positive, we do a tube screen and Auto with PEG, our backup method. If the tube screening and Auto are negative, end of story. Report screening as negative. Even Immucor says they do not know why this happens with some patients, but the most recent theory is that these patients react with the "glue" that holds the rbc antigens to the walls of the sample wells. I am very concerned about your coworker's thought process. You are absolutely correct that a newly developing antibody, or an old one with a low titer can react with the Capture method and be negative in tube. Just running tubes to "make the reaction go away" is very dangerous. Almost always, a patient with this non specific type reaction will react in all the wells of a capture strip. Good luck, Beth