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Brenda K Hutson

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Brenda K Hutson last won the day on June 26 2020

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    Blood Bank Technologist

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  1. Report as "Unable to determine Rh at this time." Brenda Hutson
  2. When I was at the Red Cross Reference Lab, we once had a donor who had donated 13 times as an O NEG.....then was determined by the regional donor testing lab to be a very weak subgroup of A (not sure of all of the testing they had performed to detect this and why it would not have been detected before this....but it was confirmed). Someone above suggested perhaps a weak A (or B subgroup)......maybe there is something to that?? Were all of the patients you tested against this unit, group O? Just a thought Brenda Hutson
  3. Well, not sure the wiggle room is "right" since no one has said anything specific, other than run positive and negative controls. So just throwing out some methods I have used in various places throughout the years. If you use the antisera method, you could easily run a positive and negative control cell for the antigen you are trying to detect or rule-out. In the other method, the cell with the known antibody would be your positive control and the rule-out cell would be your negative control (provided it was in fact negative). Brenda
  4. People have long used expired antisera and panels. You just need to run controls. Which brings one to the question....what are appropriate controls? That has never been clearly defined. Some people antigen type the cells tested for the antigen(s) for which they are using the cell (i.e. if using it to rule-out Anti-E, they type it for E to make sure it is still reactive). I was taught a variation on the theme. That is, to run a Positive Control Cell, which is a cell that is positive for the suspected/ known antibody and which reacts then at the same strength. For example, let's say you
  5. Hello Malcolm, I am the same Brenda Hutson that used to Post on PathLab Talk, but my e-mail address changed and I forgot my Password, so when I tried to login, it said Brenda Hutson was already in use, so I had to put my middle initial. Glad to be back. Brenda
  6. Hello All, I am the same Brenda Hutson that used to Post on PathLab Talk, but my e-mail address changed and I forgot my Password, so when I tried to login, it said Brenda Hutson was already in use, so I had to put my middle initial. Glad to be back. Brenda
  7. So we know that at times we add extra plasma to increase detection of weak antibodies. But my question is, has anyone done this with GEL testing? The instructions clearly state to use 25ul of plasma so just curious as to whether that is even an option with that technology? Thanks, Brenda Hutson, MT(ASCP)SBB
  8. Our new supervisor wanted me to see what others are doing with regard to questionable, weak blood types. Specifically, if for example the forward type is strong but the reverse is weak (say <2+) and there was no obvious explanation for the weak reverse type (immunodeficiency; elderly; etc.), would you still call out the blood type, or would you call it inconclusive based on not having an explanation for the weak reverse type? i.e. Anti-A=4+ Anti-B=0 A1C=0 BC=W+ Without an "obvious" explanation for the weak reverse, would you report the patient as group A or would you report it
  9. I know there must have been discussions on this topic, but in my search, only found it as it relates to young children (which is not our patient population). We are moving towards a 2nd blood draw/ blood type on patients with no historical blood type. I would be interested in hearing how others are managing that as far as workflow and hospital staff buy-in. 1. For Pre-Ops, when do you get that 2nd specimen? 2. For Outpatients, when/ how do you obtain that 2nd specimen? 3. For Inpatients they want to transfuse, what is the protocol? 4. Do you require it just be a di
  10. This refers to New Lot Confirmation and Acceptability. It states that "New reagent lots and shipments are checked against previous reagent lots or with suitable reference material before or concurrently with being placed in service. " Currently, we perform Lot to Lot Testing for the Fetal Screen Kits only. Is anyone interpreting this CAP question to also be applied to Commercial Reagent QC Kits?? Do we now need to perform Lot to Lot Testing on those also? Thanks, Brenda Hutson, MT(ASCP)SBB
  11. This refers to New Lot Confirmation and Acceptability. It states that "New reagent lots and shipments are checked against previous reagent lots or with suitable reference material before or concurrently with being placed in service. " Currently, we perform Lot to Lot Testing for the Fetal Screen Kits only. Is anyone interpreting this CAP question to also be applied to Commercial Reagent QC Kits?? Do we now need to perform Lot to Lot Testing on those also? Thanks, Brenda Hutson, MT(ASCP)SBB
  12. This is not an "exciting" question....but just wondering, does anyone out there know of a commercial label for Antigen Typing (to place on the unit) that has a space provided to write the Unit#? We could get some specially made, but that would be expensive; so just curious. I know we had one at a previous place I worked at many years ago, but not sure if they made those themselves? Thanks, Brenda Hutson, MT (ASCP)SBB
  13. So we have had 2 patient mysteries in the past week. One of them probably has a simple solution....but is just not something I have ever seen in over 30 years. The other one is more of a mystery. 1st case: We received a Cord Specimen on the baby from an A NEG mom to evaluate for Rhogam. The baby typed 4+ with Anti-A, but 1+ with Anti-B. We did wash the cells many times. We also obtained a heelstick but obtained the same results. I am used to seeing weak A typing on newborns; but not used to seeing it with Anti-B (but then statistically, I have seen many more A's over the years than
  14. For those of you who have switched to either the Erytra or Vision, I would be interested in hearing the Pros and Cons of your particular piece of equipment. I know there have been some separate listings (usually equipment-specific), so would just like to see it all laid out; which has the least problems (or the more manageable problems) as it seems they both have their pros and cons. Thanks, Brenda Hutson, MT(ASCP)SBB
  15. I am trying to create the conditions by which we can perform our Cord Blood Testing via automation. As we all know, cord blood specimens are not great. We were using a sterile screw top tube that they had used years ago, but in my efforts to see if we could automate it, we were able to locate sterile pink EDTA tubes for them to collect in. However, still getting them with clots. They found that if they filled them only 1/2 full (so quicker to get cap back on), the clotting was not as bad or frequent....but still no consistency. Our automation will run testing on Cord Specimens (Erytra), b
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