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Popular Content
Showing content with the highest reputation since 02/28/2024 in all areas
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Anti-Sciana 3
jtemple and 2 others reacted to Malcolm Needs for a topic
Vis-a vis Sc:-3 blood, I remember when I was working as a VERY junior member of staff in the IBGRL Red Cell Reference Laboratory of Dr Carolyn Giles and Joyce Poole, we did a family study following the relatives of an Sc:-3 female in a small village in Papua New Guinea (PNG), and we found six others. This was at least 40 years ago now, but it may be worthwhile contacting the PNG Blood Service to see if any of them are still donors, or, indeed, if they have found any other such donors.3 points -
Premature baby with Positive Antibody Screen?
John C. Staley and one other reacted to Michelle R for a topic
Interesting update. Received a very small amount of additional sample and performed a manual GEL (BioRad) crossmatch and it was compatible. Decided to try a 3% 3 cell screen (Immucor) converted to 1% for GEL testing and it was also negative. Very different results since we had been seeing pan reactivity in manual GEL previously (about a week ago). Possible preservative antibody that is neutralized (?) or non reactive in LISS tube? Hoping for more sample to perform repeat testing, maybe whatever it was has gone below detectable limits?2 points -
Solid Phase Methodology
John C. Staley and one other reacted to jshepherd for a topic
Hello! I've worked with Immucor over my entire 18 years in the BB - you should be able to reach out to your account rep for the info you are seeking regarding ABID troubleshooting. They have a collection of white papers that can help you. Regarding your Rh negative confirmation in tube - that sounds like something specific to your facility. It's not an Immucor recommendation that I am aware of, nor do we do that at my facility. The CMT plates are literally just a blank microtiter well, so the reactions are very similar to tube, and are read the same way. The trouble with the instrument reading the reactions is that it will not detect mixed field or rouleaux and can have false positives because of that.2 points -
BloodBankTalk: What is a “super Coombs” test?
Kris L. and one other reacted to Malcolm Needs for a topic
NOBODY has EVER performed either an Indirect Coombs Test (ICT) (or, still worse, an Indirect Coombes Test), or a Direct Coombs Test (DCT) (or, still worse, a Direct Coombes Test). There is most certainly NOT either an Indirect or Direct AHG Test. AHG is a reagent used in both the IAT and the DAT. The correct terminology for the former test is the Indirect Antiglobulin Test (IAT) and for the latter test is the Direct Antiglobulin Test (DAT). It is true that Coombs was the primary author on three papers describing the test1-3, but Mourant and Race were his co-authors on these papers, and they are often forgotten. Indeed, Coombs himself did not like the test being referred to as the Indirect Coombs Test and the Direct Coombs Test4, particularly as the principle of the test had been described in two papers published in the early 1900s,5, 6. 1. Coombs RRA, Mourant AE, Race RR. Detection of weak and ‘incomplete’ Rh agglutinins: A New Test. Lancet 1945, 246, 15-16. DOI: 10.1016/S0140-6736(45)90806-3. 2. Coombs RRA, Mourant AE, Race RR. A new test for the detection of weak and “incomplete” Rh agglutinins. British Journal of Experimental Pathology 1945; 26(4): 255-266. 3. Coombs RRA, Mourant AE, Race RR. In vivo isosensitization of red cells in babies with haemolytic disease. Lancet 1946; 247: 264-266. DOI: 10.1016/S0140-6736(46)91925-3. 4. Coombs RRA. Historical note: past, present and future of the antiglobulin test. Vox Sang 1998; 74: 67-73. DOI: 10.1046/j.1423-0410.1998.7420067.x. 5. Moreschi C. Neue tatsachen über die blutkörperchenagglutination. Zbl Bakt 1908; 46: 49-51. 6. Friedemann U. Weitere untersuchungen über den mechanismus der anaphylaxie. Z Immunitätsforsch Exp Ther 1 Originale 1909; 2: 591-641 (cited in reference 4).2 points -
Antibody ID
Yanxia and one other reacted to John C. Staley for a topic
Most assuredly!! She's a nurse and would not allow such activity unless performed with the upmost professionalism!2 points -
Antibody ID
Yanxia and one other reacted to Malcolm Needs for a topic
With a syringe and cannula I trust My Good Sir!!!!!!!!!!!!!!!!2 points -
Antibody ID
Malcolm Needs and one other reacted to John C. Staley for a topic
Not many have my option. My wife has an Anti-D, an Anti-K and for a short time a detectible Anti-s. I would just draw some of her blood every so often.2 points -
Antibody ID
Yanxia and one other reacted to Malcolm Needs for a topic
In the Reference Laboratory I worked in (in the UK, NHSBT-Tooting Centre in London), we would freeze any useful samples from both patients and donors (although the latter were quite rare), but we also belonged to a scheme named SCARF (Serum, Cells And Rare Fluids). I must confess that, having retired in late 2016, I'm not certain that SCARF is still going, or, come to that, how much it costs to join. One thing that I would caution against, and that is diluting a "strong" antibody to make a "weak" antibody (although this is far more important when trying to make a "weak antibody" to use as a control (for example, for an IAT), as a "strong" antibody has a completely different equilibrium constant (although this may not be too important if you just use them for teaching).2 points -
Premature baby with Positive Antibody Screen?
Yanxia reacted to Michelle R for a topic
We were thinking an anti-I initially as well but we didn't find much literature about a premature baby forming an anti-I at birth since it appears to be IgM. We were also wondering about other possible antibodies that would be non-reactive or weaker with cord cells. We requested Immunoglobulin tests and received the results today so I will post them for additional information. IgM is double the upper value for the range?? IgM= 45 mg/dL (range for <1 month old is 4-20 mg/dL); they repeated to confirm IgM= 47 mg/dL IgG= 519 mg/dL (range for <1 month old is 649-1627 mg/dL) IgA= <6 mg/dL (range for <1 month old is 0-0 mg/dL)1 point -
Imelda Bromilow.
Malcolm Needs reacted to Neil Blumberg for a topic
My condolences to her family, colleagues and friends.1 point -
Antibody ID
Yanxia reacted to Malcolm Needs for a topic
I confess that I am no expert on the chemistry of this, but, as I understand it from the late Prof. Patrick Mollison's book, it is purely to do with differences in the equilibrium constants of weak and strong antibodies.1 point -
Hi @Pamo, There are a few options. If you subscribe to a proficiency testing program, you can often save what they send you. WARNING - do not use these samples until after you have received the results if you are a CLIA lab. You can spike your training samples with commercial antisera. These don't always react as expected / hoped for. In my opinion, the best option is to save a recent prior patient sample that is no longer needed or expired. Commercial samples are also available, such as this from Hemo bioscience.1 point
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Imelda Bromilow.
exlimey reacted to noelrbrown for a topic
Thanks for letting us know, I worked with Imelda on a couple of projects when she was at Liverpool BTS. I always enjoyed her humour and approach to life. Noel1 point -
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My old lab used to do something similar! Just a dummy slide with the probe wedged in the well and putty sealing around the opening. For those wondering if a thermometer will fit -- there are some that do, and the wiring threads out under the cover using a small notch1 point
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Temp indicators for blood products
Kelly Guenthner reacted to RRay for a topic
This is our process except we don't do a data logger with each cooler. The indicators are considered an FDA approved device so we don't do both. We don't use them on every unit, just cooler units, and use the temp gun upon return. I loathe the indicators and I think they are borderline worthless. But I'm a bit gunshy of not using them for coolers after being cited many years ago. That was when there was that hot debate over coolers being transport vs storage. I was told at that time that the temp gun alone wasn't adequate because it doesn't detect if a unit was out of temp and then back in the cooler long enough to acclimate.1 point -
Ortho Gel combo workstation
SbbPerson reacted to Kelly Guenthner for a topic
1 point -
We document that the light is indeed green, indicating that the factory set temperature is OK, on our Daily Temperatures and Checks form as a check mark each day for the "MTS Incubator Status Light Green?". We also check the RPM's daily and record on the same form.1 point
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🩸Delve into the complexities of transfusion management with Magrolimab, an anti-CD47 monoclonal antibody. Explore achievements, challenges, and groundbreaking insights, emphasizing crucial mitigation strategies. Navigate the world of Magrolimab and its impact on RBC typing and serological testing. Discover the future implications and suggested avenues for research in this topic.📚🔬 https://immunohematologymadeeasy.com/summaries-and-mind-map-transfusion-management-in-the-era-of-magrolimab-hu5f9-g4-an-anti-cd47-monoclonal-antibody-therapy/1 point
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Changes to Manufacturer's Inserts
AMcCord reacted to BankerGirl for a topic
We use MediaLab also and scan the package inserts in a job aids. You can revise them the same as you can your policies.1 point -
Using platelets returned in a cooler with ice
Ally reacted to Neil Blumberg for a topic
Here's one paper that involves extended cold storage of room temperature platelets. They actually seemed more functional. Xu F, Gelderman MP, Farrell J, Vostal JG. Temperature cycling improves in vivo recovery of cold-stored human platelets in a mouse model of transfusion. Transfusion. 2013 Jun;53(6):1178-86. doi: 10.1111/j.1537-2995.2012.03896.x. Epub 2012 Sep 24. PMID: 22998069. Background: Platelet (PLT) storage at room temperature (RT) is limited to 5 days to prevent growth of bacteria, if present, to high levels. Storage in cold temperatures would reduce bacterial proliferation, but cold-exposed PLTs are rapidly cleared from circulation by the hepatic Ashwell-Morell (AM) receptor, which recognizes PLT surface carbohydrates terminated by β-galactose. We cycled storage temperature between 4 and 37°C to preserve PLT function and reduce bacterial growth. Study design and methods: Temperature-cycled (TC) human PLTs were stored at 4°C for 12 hours and then incubated at 37°C for 30 minutes before returning back to cold storage. PLTs stored at RT or at 4°C (COLD) or TC for 2, 5, and 7 days were infused into SCID mice and the in vivo recovery was determined at 5, 20, and 60 minutes after transfusion. Results: PLTs stored for 2 days in COLD had significantly lower in vivo recoveries than RT PLTs. TC PLTs had improved recoveries over COLD and comparable to RT PLTs. After 5- and 7-day storage, TC PLTs had better recoveries than RT and COLD PLTs. PLT surface β-galactose was increased significantly for both COLD and TC PLTs compared to RT. Blocking of the AM receptor by asialofetuin increased COLD but not TC PLT recovery. Conclusion: TC cold storage may be an effective method to store PLTs without loss of in vivo recovery. The increased β-galactose exposure in TC PLTs suggests that mechanisms in addition to AM receptors may mediate clearance of cold-stored PLTs.1 point -
Judd’s Methods in Immunohematology, 4th edition
kmbloodlz reacted to Sherif Abd El Monem for a topic
🩸🔬 Exciting Update in Immunohematology: Introducing the Fourth Edition of "Methods in Immunohematology"! In this latest edition, you can look forward to: 🔹 Unveiling new and advanced techniques 🔹 Enhancing your procedural finesse 🔹 Ensuring seamless and secure transfusions From conquering antibody detection to mastering ABO typing challenges, this guide is your comprehensive roadmap in the world of immunohematology. With the combined experience of over 50 years, these authors are truly your go-to companions for all things blood transfusion-related. 🏥🩸 https://immunohematologymadeeasy.com/judds-methods-in-immunohematology-4th-edition/1 point -
Ortho Panel A and B quality control
chupert reacted to John C. Staley for a topic
In all my years (30+) in blood banks and transfusion services I never QC'd panels and it was never addressed in any inspections/assessments. When ever the subject came up I figured that if you were not QCing every antigen on every cell you were doing little more than providing some random inspector with smoke and mirrors so they would think you are doing something worth while. A some point you need to trust the manufactures to do their job.1 point -
Just to add to this, ask your medical director if he/she is willing to help you out with study material. At the University Hospital where I work the medical director was kind enough to take us through the same material/coursework that the residents go through in order to prepare for their board examination. That along with a lot of studying on your on will have you on your way to an SBB.1 point
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Qualifying for the SBB
applejw reacted to David Saikin for a topic
You do not have to go to SBB school - BUT, the test is made so that those who do attend will have a better chance at passing. Challenge it . . . I did. Remember, this specialty exam has the least percentage of techs passing, but look at the knowledge you will gain in studying for it. Also, some blood centers offer study courses and I think there are some on line also. good luck1 point -
Imelda Bromilow.
John C. Staley reacted to Malcolm Needs for a topic
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