So, I did a Google search, and this was first on the list for 2 cell vs 3 cell antibody screen.
We have been doing a 2 cell screen for over a decade, I was hoping to find a published study that indicates this is unsafe and we are missing antibodies. To me, it intuitively feels like we should be.
We are using BioRad. I am neither knocking nor endorsing any company, we have two IH-1000 and those are the cells we use.
Their 2 cell screen says:
R1R1 (CDe/CDe) and R2R2 (cDE/cDE)
Homozygous expression of Jka
Their 3 cell screen says:
R1R1 (CDe/CDe), R2R2 (cDE/cDE), and rr (cde/cde), Jk(a+b-), Jk(a-b+), Fy(a+b-), Fy(a-b+), M+N-, M-N+, S+s-, S-s+, Le(a+b-), Le(a-b+)
How are we not missing weakly reacting Fya, or Fyb, or Jkb...? The two cell screen is only (planned) to be homozygous for Jka.