jtemple

What? All this time I have been using the wrong stuff! Ha! SPELL CHECK IS NOT YOUR FRIEND! 🤣🤣🤣🤣

I have never understood why we are using DDT instead of trypsin? I do realize that DDT is somewhat easier to use than trypsin, but the Kell problem is eliminated. M & N require looking at, but I will take that trade. In the Southeast we also look out for Jsb as quite a few Jsb- people live here.

You are probably right, however these are to get us within the transport requirements that are imposed on us by regulatory agencies.

Seems like a bit of insulation should take care of it. Not exactly sure what that would look like though. But something to slow down that temperature change. I usually sandwich mine in a couple of refrigerated cooler bags.

The interpretation we use is if the patient is actively bleeding then yes we give them. If not we turn it over to the medical director to decide. We then just document and abide by thier decision. They decide after consult with the attending and lab values.

That's an awfully big question and I am going to "try" to hit the highlights. PLEASE feel free to add or clarity anything to keep my foot out of my mouth! I will start with the standard Dat is positive. You perform an eluate and it is negative. That points to the possibility of a drug based reaction. It's the policy of some facilities to not repeat the elution if the strength of the dat has not increased in strength or a new allo antibody has been created. The elution can also help you determine if it is truly a auto or an allo antibody by seeing if the eluate reacts with DAT negative auto cells (retic harvested if needed of course). Next, what about the times when the DAT is negative? The auto could be be directed at something that is surpressed or even negative. The antibody might even be a different class of antibody such as Iga for example or perhaps a subclass of IgG that your antisera doesn't pick up. These are just a few things that I will note, quickly. Perhaps we can get Mr Needs to make a few comments. Jason Temple

The ARC guide that we use say that return of csp are guided by similar conditions for blood product return.

Recently, I had a situation that bothered me and I wanted to reach out to this knowledgeable group for opinions. A patient was going into the organ donor process. They wanted 4 or 5 units of blood. Unfortunately, the patient was O Negative and I could only offer them 2 units. So, the group handling it contacted a blood supplier and got three additional units and brought them into my hospital. Then proceeded to use them during the course of the process. My first question, should these units come through my blood bank? How can they not have these units tested at all? No ABO recheck, no crossmatch, nothing. Now according to them as the patient has passed, they can do that. I can find no exception in the standards. Is there something I don't know?

This is EXACTLY what I do. That way you can continue your juggling act without worrying about what wash you are on!

I haven't seen a rebuttal but the FY system is reported to be a problem with storage. It was reported by : Williams D, Johnson CL, Marsh WL. Duffy antigen changes on red blood cells stored at low temperature. Transfusion. 1981 May-Jun;21(3):357-9. doi: 10.1046/j.1537-2995.1981.21381201813.x. PMID: 7233520.

Harbor freight mini files are your friend.

I may be way off base, but this sounds like a drug reaction.

The biggest offender in this scenario is not letting your reagents come to room temperature before testing. Waiting at least 15 minutes after taking reagents out of the fridge will avoid a lot of this. If that is not the case here, perform a DAT on the unit.

Hi all, Community Blood Center of Greater Kansas City has one of these for sale. Please contact me if interested at jtemple@cbckc.org Jason Temple MT(ASCP)SBBcm