Rapundaa

We have been using the Galileo and Neo for 12 years. Yes we have seen issues with the Rh typing being positive on previously negative patients. Our protocol is similar to Dothandar.

Scott- What methodology are you using (gel, solid-phase?) We see a lot of equivocals with solid-phase and it can be very frustrating to weed through the reactivity to discover if a clinically significant antibody is there. We also see more equivocals in certain categories of patients (pregnant and some auto immune disease states). And as Malcolm has stated we have seen some of these equivocals become actual an actual E, C, Jka and several others! Sometimes going to an alternate method (we go from solid-phase to gel) can help because a patient may have a lot of non-specific reactivity in one method and none in another. Not the most definitive answer but we share your pain!

Same here since about the same year.

We are a Level 1 Trauma Center with extensive emergency, surgical, obstetrical, and outpatient services. Looking for motivated technologists, experience is a plus! Currently we have open positions for a full-time midnight shift and a part-time afternoon shift. These are dedicated blood banking positions (no rotation in other lab sections). The hourly rate is highly competitive for this regional area, excellent benefits as well. Our hospital is in Ann Arbor, Michigan. Please specify that you are interested in the Transfusion Service positions when completing the application using the link below: http://www.sjmercy.org/careers

We are a Level 1 Trauma Center, with extensive surgical, obstetrical and outpatient services. Looking for a motivated technologists, experience a plus! The open positions are for a full-time midnight and a part-time afternoon shifts. These are dedicated blood banking positions (no rotation in other lab sections). Our hospital is in Ann Arbor, Michigan. Please specify that you are interested in the Transfusion Service positions when completing the application using the link below http://www.sjmercyhealth.org/careers

In this case the donor will not be "on site". There is a surgery facility where the harvesting takes place and they would like us to provide units to keep the donor oxygenated during the process. The donor is anonymous and we will have no prior txn history.

Could you footnote (non-chartable) the control results on the antibody screen for manual testing? We use Cerner PathNet and we document control testing for antigen typing in the non-chartable section. We Keep manual testing results in a folder with the patients name (as for antibodies) and scan those into a special on-lie folder once every two to three years.

Remember that turn-around-time is based on how many specimens are running in advance of the new ones being added for the next run. Also remember that some analyzers will not allow you to add additional specimens if testing is already in progress (not continuous access). It's helpful to be allowed to cancel testing that has not already started (without loss of reagent volume) and add STAT testing.

Does anyone have experience providing blood to a gift of life organ donor? Do you receive a specimen and provide compatible blood based on serological testing or do you issue uncrossmatched O negative units? Have you ever experienced a transfusion reaction with this situation?

We considered doing this recently but because we are FDA inspected we didn't want to get into the whole "home made reagent" issue. There are a lot more hoops to jump through unlicensed sera.

I'm not entirely sure what you are asking... But why was antibody identification not done in solid-phase? Kidd antibodies are known to love the solid-phase environment, gel not so much. We have had numerous examples of Kidds that react in Capture and not in gel during our validation testing and since. It is always a best practice to be performing your identification in the same method as your screening. And that would extend to crossmatches as well.

We accept verbal orders in emergency situations. The expectation is that these orders will be entered at a later time into the HIS (mostly happens). The request is documented on one of these forms (attached) and it is stapled to a copy of the checklist that we use during issue of products. This form and checklist are stored with all the other Blood Product Request forms received via the LIS for 10 years. Request for Issue.pdf

The following are our criteria for performing a KBT: Order a KBT on an Rh-negative obstetrical patient for any of the following: post-delivery Fetal Screen test is positive, trauma during pregnancy (ordered by the physician), mother had a vaginal bleed, fetal death, terminated pregnancy, amniocentesis or CVS at > 20 weeks gestation, mother had a version procedure, and/or neonate is Rh-negative with a positive or invalid Weak D test, or Rh cannot be determined. At less than 20 weeks we issue one dose of RhIg without further testing, other than the type and screen. CarrieM has nicely summarized the use of the Immucor FMH RapidScreen if that is the kit you are using. Additionally at delivery, we use an on-line calculator recommended by the AABB to determine the amount of RhIg to be given based on the mothers height/weight: W:\AB\Bloodbank2\CAP AABB RhIG calculation\RHIGCALe.zip\

We have NEOs and the positive and negative controls are part of each set of AHG-xms run on the analyzer.

Same as ChrisW for 10+ years