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Tabbie

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Tabbie last won the day on May 12

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    BMS1

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  1. Tabbie

    Mycoplasma pneumoniae

    Thanks Galvania All made up so if you don't ever see something I have 'made up' please let me know. I was trying to fit lots of questions into one scenario.
  2. Tabbie

    Mycoplasma pneumoniae

    Thanks for your time yes very helpful. I will keep read
  3. Tabbie

    Mycoplasma pneumoniae

    So for example the question below If you only knew that the patient had been transfused but no other details how would you phenotype or would you just obtain a genotype? The BSH guidelines say " If the patient is known to have been transfused in the previous three months, phenotyping may be misleading" Most regularly transfused patients such as Haemoglobinopathy patients admittedly would already be phenotyped but if they were not they would required Rh K matching units, DARA patients would you Rh phenotype if units are required ASAP but you have time for a crossmatch. What further tests would you do if you had the resources to ensure you provided matched units So for example after the elution to identify the antibodies can you use the RBC left from the process to phenotype ? or does the elution damaged the RBC is there another procedure for phenotyping if the patient has been recently transfused? Misleading means ? - What anomalies would you actually see ? weakened reactions, dual populations (if ABO) anything else? Why with warm autoantibodies does elution not help ? What about in pregnancy are there any anomalies with elution/adsorption/phenotyping ? Thanks
  4. Tabbie

    Mycoplasma pneumoniae

    I work in a small lab we just don't get the experience of the unusual. I am trying to learn more by posting hypothetical case studies as I cant post real ones. Thanks
  5. Tabbie

    Mycoplasma pneumoniae

    Great thanks Galvania More questions If it was an anti-I would the pattern be pan-reactive by IAT weaker and Enzyme stronger reactions? If patient was previously transfused would you phenotype RBC if you knew the group of the ABO mismatch i.e. if a dual population say Group A given to a group O? Would you perform an eluate to remove the antibodies then add anti-A to remove the A RBC then phenotype the O RBC and assume they are the patients RBC as the phenotype? If you only knew that the patient had been transfused but no other details how would you phenotype or would you just obtain a genotype? If no mix field and DAT C3d only and Donath Landsteiner test was positive would you then titer with P antigen positive RBC? Cheers
  6. Tabbie

    Auto adsorption

    Thanks Malcom for reply it was a while ago and I didn’t cite it as I thought I would come across more that would explain it. But looks like from you reply this is not possible. If I come across it again I will post.
  7. Tabbie

    Mycoplasma pneumoniae

    Hi All Hypothetical Senario Female patient unknown transfusion history with mycoplasma pneumoniae what exclusions and further testing would you perform ? When would you perform a titre ? Reactions greater than 3+. If emergency units required with titre greater than 64 what is your protocol ? Thanks
  8. Tabbie

    Lewis exclusions

    Hi All Lewis antibody exclusions. Realise they are rarely clinically significant only causing HTR and would perform crossmatch compatible. However I want to clarify the theory for exclusions. Human Anti-Lea is found in Le(a-b-) however exception to rule in Tawain where 10 Lea (a-b+) patients with anti-Lea were found. Most anti-Lea contains weak anti- Leb causing false positives. However monoclonal reagents seem to indicate no cross reactivity? What reagents do you use to exclude the presence of either anti-Lea or Leb ? Thanks
  9. Tabbie

    Auto adsorption

    Hi All Reading about an auto anti-C with C antigen negative expression by serology phenotype and genotype. If you could not obtain a result from auto adsorption (not enough sample to test) how would you confirm it was an auto antibody ? Thanks
  10. Tabbie

    NAD but positive Cross-match

    I like things to be right keep up the good work of correcting and educating 😀
  11. Tabbie

    NAD but positive Cross-match

    Patient phenotype is Fya + Fyb+ I just had not seen a Gata mutation result before had read about it. Thought it may be an additional molecular phenotype request but it was included in the panel reported
  12. Final results Auto Anti-Jka and nonspecific enzyme IAT ( no requirement to select Jka antigen negative units) Phenotype E-K- and again Fy GATA mutation negative. Has anyone seen an anti-Jka only react by enzyme and not IAT which has not been an auto anti-Jka ? Thanks
  13. Tabbie

    NAD but positive Cross-match

    Final results ABO group not determined. Phenotype c-E-K-M+N- and interestingly Fy GATA mutation negative.
  14. Tabbie

    What are your rules for ruling out?

    Are there any rules for ruling out P1 with the different antigen expression strengths? Thanks
  15. Tabbie

    NAD but positive Cross-match

    Interesting about Lea+Leb+. The eluate was negative
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