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Tabbie

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Tabbie last won the day on May 12

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    BMS1

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  1. Tabbie

    NAD but positive Cross-match

    I like things to be right keep up the good work of correcting and educating 😀
  2. Tabbie

    NAD but positive Cross-match

    Patient phenotype is Fya + Fyb+ I just had not seen a Gata mutation result before had read about it. Thought it may be an additional molecular phenotype request but it was included in the panel reported
  3. Final results Auto Anti-Jka and nonspecific enzyme IAT ( no requirement to select Jka antigen negative units) Phenotype E-K- and again Fy GATA mutation negative. Has anyone seen an anti-Jka only react by enzyme and not IAT which has not been an auto anti-Jka ? Thanks
  4. Tabbie

    NAD but positive Cross-match

    Final results ABO group not determined. Phenotype c-E-K-M+N- and interestingly Fy GATA mutation negative.
  5. Tabbie

    What are your rules for ruling out?

    Are there any rules for ruling out P1 with the different antigen expression strengths? Thanks
  6. Tabbie

    NAD but positive Cross-match

    Interesting about Lea+Leb+. The eluate was negative
  7. Hi All Female patient not childbearing age with historical A POS no antibodies detected subsequently multi transfused. After transfusion of 2 x O NEG units a week later a sample tested no antibodies detected by IAT however cross-match request was performed by IAT and both units were positive Further testing DAT +1 and panel cells enzyme ONLY reactive +2 with R1R1 cells and the rest of the panel cell reactions +4 ABO reverse grouping showed A cells +2 (conclusion therefore possible A subtype with a no specific antibody or reaction with cde in reverse grouping reagents due to an anti-c) Reference Lab Results Non specific anti-E and anti-c detected in enzyme IAT, DAT IgG 1+ As multi transfusion advise was cross-matching group O E-c-K- red cells Genotyping results to follow. Would anyone agree with the reactions with the reverse grouping agents and if they have seen this was the anti-c detected been by IAT/and or Enzyme Thanks
  8. Hi All Historical A POS (never phenotyped) no antibodies detected. MDS patient, multi-transfused with APOS/NEG units over past 3 months. Results Screening cells (using BioRAD 3 cells) +2 positive reaction only with R2R2 (cell II). DAT +2 (IAT method/ IgG/C3d polyspecific) Panel negative which suggested a low prevalence/frequency/incidence antibody however - crossmatch was positive which led the question was there was another underlying antibody ( a soup of antibodies). Reference Lab Results Possible Jka by IAT enzyme and non specific enzyme reactions. Now awaiting genotyping. Advise to crossmatch Rh/K/Jka D-C-E-Jka- My question is now the patient has returned to group A POS (there was a dual population before with the D type due to the previous transfusions) is it acceptable to transfuse D+ (as historically RhD+) C-E-Jka- units or could there be a potential D typing anomaly ? Thanks
  9. Tabbie

    Elution Studies

    Can you expand on the IgG 2 and/or IgG4 rational/theory ? Was the DAT negative for these patients and can you give an explanation to why ? Thanks
  10. Tabbie

    Elution Studies

    In regard to the low affinity antigens it seems that DAT are usually negative as described because these antigens do not fit well with their cognate antibodies and HDFN/HTR is rare. Can any distinction be made in regard to positive/negative DAT results for high prevalence antigens ? The 901 series seems to cause severe HDN/HTR (Anti-Vel) and MAM describes DAT + but no HDFN with IgG1 and IgG 3 ? Thanks
  11. Tabbie

    Rh phenotypes

    What if the emergency o negs are taken for a patient with an anti-c antibody ? What protocols do you have for patients with antibodies who maybe are from another hospital (not on your system) and want for example group specific uncrossmatched ? Do you use concession/declaration forms before they take the O negs/group specific or can they just take them ? Thanks
  12. Tabbie

    Elution Studies

    Good points I used the wrong description. Can you clarify the use of frequency and incidence are synonymous with prevelance. Assuming so and that the 700 series is different collection only because they do no belong to any known blood group system ? Thanks
  13. Tabbie

    Reagents

    Does anyone use Alsevers instead of Cellstab and why which test would it be used for ?
  14. Tabbie

    Reagents

    Now reading the JPAC 1.2: Specifications, performance evaluation and quality control of blood grouping reagents and lab reagent inserts 😀 there seems to be a mix of human and murine products just wondering for example why anti-Lea is made from murine IgA as it’s biochemistry is built on type 1 chains can a human equivalent not be made ?
  15. Tabbie

    Reagents

    Thanks for all that detail much appreciated I will read about auto anti-E Happy Easter 🐣
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