By Eagle Eye
Do you check microscopic reaction for all DAT?
Do you use Ortho/Immucor/Other reagent?
Do you receive specimen fro API/CAP for proficiency testing?
Is there a requirement to check microscopic reaction / mixfield reaction?
If yes, is this CAP/AABB/CLIA/other? \
Our facility is evaluating making a change to our process for Weak D testing for patients with a positive DAT. For years, if we were required to do a Weak D, but the patient had a positive DAT, we used to cancel the Weak D as invalid. Another hospital in our system mentioned that they tended to perform the Weak D, but then only cancel as invalid if the Weak D is positive. We are thinking about changing to this process, as we now have to result many babies as "Rh Unknown" and give their mothers Rhogam.
Per our Anti-D's package insert: "Red blood cells coated with alloantibodies or autoantibodies of the same or similar specificity as the reagent (i.e. cells that are DAT positive) may give weak reactions. This is due to decreased availability of antigen sites because of antigen blocking or steric hinderance. In extreme cases false-negative results may occur."
I'm worried about the "extreme cases" where a false negative could occur, but I cannot see this being common. Also, would you think that if the cells were coated with that much antibody, that we would see any other odd reactivity in the ABO/Rh? What do other facilities do?
Thank you in advance,
I am sorry if this has been discussed previous... I searched and didn't find anything. Quick question...
We are transfusion service that performs DAT's using poly-clonal IgG... if it is positive, we run the mono-clonal IgG, however, we do not run the C3d. How many of you would and/or do run the complement control cells for DAT QC in addition to Check Cells?
We are about to move from using Bio-Rad IH-1000 to Immunocor NEO in our blood bank department. As most of you are already aware, the IH-1000 uses column agglutination technology (CAT), whereas the NEO uses Solid Phase Red Cell Adherence (SPRCA) assay. SPRCA is known to be more sensitive, which is great when picking up on elusive antibodies belonging to Kidd blood group system (I think ). My concern is about the techniques which employ the use of indicator cells that are coated with anti-IgG. It will only pick up on IgG antibodies and none of the IgM antibodies. How significant is this? Is there any way of picking up IgM antibodies using such technique? Or should we not worry since IgM antibody does not usually reaction at 37C?