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Yanxia

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Everything posted by Yanxia

  1. The elution react with the father's red cells. Using those three Rh types, I reference auto-antibodies adsorption, because I am not sure if there is a mix of anbodies in the mother's.
  2. Maybe we can try by using the mother's serum to react with the father's red cells (if they are not ABO type compatible, using R1R1,R2R2,rr red cells adsorb the allo-antibodies separately before doing elution ). I am not sure if I can make myself clear.
  3. I just can't understand why the phenotype gives 4+ of c antigen when the genotype is c neg.
  4. Thanks for sharing, Andrea. I have spotted one big difference between your SOP and mine, that is we don't discard the pipette tips for each dilution. I will do paralle test to see the difference in titre later.
  5. Maybe some day we can actually do it, My meaning was if we detected an antibody and didn't make sure it was auto or allo, we did crossmatch using a method that was sensitive, in case we missed it.
  6. I guess the solid phase is more sensitive to detect the auto in this patient. I will use the same method as detecting the antobodies to do crossmatch. Of course I will do saline and AHG crossmatch as well. Just my humble opinion.
  7. I am kind of curious about is the crossmatches and ABID using the same method?
  8. I guess if there was carry-over it is the same as using the same tube during the whole washing process.
  9. I mean the freezing and thawing test.
  10. Would you explain it more specifically, please? It sounds new to me.
  11. Is can detect anti-Vel which isclinical significant.
  12. The elevated IgM in the baby means there was intrauterie infection.
  13. It seems like allo- anti-I. But I can't explain why the Liss reaction is neg. I am waiting experts to explain and learn from them.
  14. May you kindly give us more details about it? Is it about the ionic strength? Thank you.
  15. Maybe repeat ABO typing can prove it.
  16. It needs about 6 minutes to expel the substance in blood circulation through urine.
  17. Just my guess, is there any possibilities of fetus D pos cells bind partof the antibodies?
  18. We have encountered a patient who produced auto anti-A(or A1,sorry I didn't identify it).
  19. Sorry,I can't find the English version.The underlined sentence means the cord blood cells needed to be washed at least once before testing.
  20. I am so lazy that I just checked our IgG card today . I noticed it said that cord blood need to be washed before testing. I am at home now, I will attach the package insert tomorrow.
  21. I guess this may be caused by the Wharton's Jelly which can cause rouleaux formation of the cord cells. For the gel technique, it is good to test the adult cells without washing, but for the cord cells, maybe the Wharton's Jelly block antigens on the cells' surface. I noticed that patients suffer from Multiple myeloma will not show false positive reaction in gel, but saddly I have not confirmed if there are false negative reactons.
  22. I prefer the each individual one policy. Because we can not make sure the baby has the combined antigens.
  23. Thanks for your sharing, Malcolm. In the course of reading the cases above, I encountered many problems which resolved by checking on books. This is a very impressive way to absorb new knowledge. As a hospital blood bank worker, I rarely have chances to get access to thoes cases.
  24. Here is a follow up. The baby had tested with anti-A on 27, April and transfused with O washed cells the same day, but on 10, May she had no anti-A and received A type packed red cells with no transfusion reaction. She has stoped feeding on her mother's milk for 14 days. I tried to persuade her mother to do some tests. The results came out and she has no anemia, with normal reticulocyte count and percentage. Her bilirubin and LDH are normal too. But she has not tested her haptoglobin. She has not received any blood transfusion and IVIG. She just diagnosed with slight anemia during pregnancy and after birth. She had taken iron supplements during pregnancy,. This is her eventh pregnancies and the third baby, she had several miscarriages. She told me her case seems like a mystery both to her and the doctors she know. She just want to know if she is ok and the baby will be healthy in the future.
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