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GEL DAT CONTROL


Davidwa
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Yes. We use poly cards and IgG cards. Negative control can be one of the screen cells. We make the DAT Positive control by incubating 1ml of O Rh pos cells with one drop of anti-D, mix well and incubate for 10 min at room temp. Don’t wash and make a 0.8% solution and use. It should give about a 2+ reaction (the objective), though this probably depends on the anti-D so you may need to experiment. 

Hope this helps. 

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We use a RH + and Rh Neg 0.8% suspension from donor units mixed with low titer Anti-D for our +/- diluent control for our IgG cards. 

We make a 0.8% suspension out of our Coombs Control cells for a Positive control and one of the above 0.8% suspensions for our DAT Controls for our IgG/C3d cards.

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  • 1 month later...

Has there been any questions regarding the manufacturer's QC instructions for polyspecific gel cards if you are only testing with IgG coated red cells?  I thought I understood from the Ortho instructions for QC that a positive and negative control were required and C3 coated cells must be used to verify anti-C3 reactivity.  I'm not saying that is what we are doing but the question has come up in our BB leader meetings about QC for the poly DAT gel cards on the Vision.

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We use a screening cell for the neg control and Ortho Coombs Control (made to 0.8%) as the pos control for both poly and IgG Gel cards.  We only perform IgG and Poly DAT's.

Edited by ANORRIS
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On ‎02‎/‎21‎/‎2019 at 6:53 AM, Malcolm Needs said:

Yes, but the card is European.  I have no idea if it is available in the USA.

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I love this card.  Had the opportunity to try it (thanks Malcolm).  Too bad it's not available in the USA.

I do not usually run a control with my IgG cards.   When I test w anti-C3b,-C3d I use my A2 cell as a negative control/C3 sensitized cells as positive.

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  • 2 weeks later...

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