Everything posted by Davidwa
Can you tell me where to buy Glycine soja lectin to prove the effectiveness of enzyme treated red cells. One company did not sell this product kit anymore. Doc1.pdf
1. In the book Judd's Methods in Immunohematology, auto adsorption is not feasible if pt transfused in 120 days. but in AABB tech manual method, the cut off is 3 months, i.e., 90 days. Would 90 days acceptable for auto adsorption? or have to stick to 120 days? any input? 2. AABB method implied that the typing on patient's reticulocytes by harvesting autologous red cells should be exercised with caution for interpretation of the E, e, c, Fya, Jka, and Ge antigens. How can be confident to the typing results for those antigens just mentioned? If no confidence, this method is not valuable to type the pt recently transfused. The reason I asked is that I eagerly to find ways to use pt typing information to assist allo adsorption. 3. I can use genotyping information, but it will take 2-3 days to get results and we may not wait so long in some cases.
Lets say you weld the donor unit with a syringe or an aliquot bag. Is this welding method regarded as sterile? What is the parent bag expiration date after taking small amount of blood from the parent bag after heat seal? No change or shorter date? What is the expiration date of aliquot / syringe after blood drawn into it (weld first, then heat seal)? 24hrs or same as parent bag?
I am trying to introduce allo absorption technique to our blood bank, due to high incidences of WAA patients with chronic transfusion dependence. Currently we have to send all these recently transfused patient samples with WAA to reference lab. Now we are exploring the possibility to do allo absorption in house. I am not sure how far we can go, really. But I hope we can accomplish it if I can get help and support from your guys with plenty of experience on allo absorption. 1. The top challenge is how to get donor cells with R1R1, R2R2, rr with combination of Kell, Kidds, Duffy positive/negative cells. If you find a donor unit meeting your requirements, how did you get the pack cell (say 5 ml or 10 ml) from the main unit bag? Cut segments? or draw the cells from the main bag then seal it? 2. if draw cells from the main bag, how many days for the main bag valid for future testing ? the original expiration date or does not matter since it was not meant to for patient transfusion? 3. or can we subdivide the whole bag into multiple small tubes or transfer bag? how to store these aliquots? expiration date? 4. any reference (literature and websites) and SOP can share? 5. Triple absorption is least I want to perform if I have patient phenotype or genotype information. That will make easier to do allo absorption. Some people said geno type should not be treated as phenotype unless phenotype confirmation performed. Not sure this is main school of thoughts or not. 6. Finally, is there any company providing commercial triple absorption cells in the market? Panel cells not feasible to do absorption right? Any help for any above question highly appreciated.