Davidwa Posted February 20, 2019 Share Posted February 20, 2019 Do you run control for the gel DAT test? If yes, what gel card you use and how to do that? If not, how to make sure the positive gel DAT is not false positive? Thanks. Link to comment Share on other sites More sharing options...
Malcolm Needs ★ Posted February 21, 2019 Share Posted February 21, 2019 Yes, but the card is European. I have no idea if it is available in the USA. jojo808, Arno and Texas Lynn 3 Link to comment Share on other sites More sharing options...
Ensis01 Posted February 21, 2019 Share Posted February 21, 2019 Yes. We use poly cards and IgG cards. Negative control can be one of the screen cells. We make the DAT Positive control by incubating 1ml of O Rh pos cells with one drop of anti-D, mix well and incubate for 10 min at room temp. Don’t wash and make a 0.8% solution and use. It should give about a 2+ reaction (the objective), though this probably depends on the anti-D so you may need to experiment. Hope this helps. Link to comment Share on other sites More sharing options...
Patty Posted February 21, 2019 Share Posted February 21, 2019 We use a RH + and Rh Neg 0.8% suspension from donor units mixed with low titer Anti-D for our +/- diluent control for our IgG cards. We make a 0.8% suspension out of our Coombs Control cells for a Positive control and one of the above 0.8% suspensions for our DAT Controls for our IgG/C3d cards. Link to comment Share on other sites More sharing options...
applejw Posted April 18, 2019 Share Posted April 18, 2019 Has there been any questions regarding the manufacturer's QC instructions for polyspecific gel cards if you are only testing with IgG coated red cells? I thought I understood from the Ortho instructions for QC that a positive and negative control were required and C3 coated cells must be used to verify anti-C3 reactivity. I'm not saying that is what we are doing but the question has come up in our BB leader meetings about QC for the poly DAT gel cards on the Vision. Link to comment Share on other sites More sharing options...
ANORRIS Posted April 19, 2019 Share Posted April 19, 2019 (edited) We use a screening cell for the neg control and Ortho Coombs Control (made to 0.8%) as the pos control for both poly and IgG Gel cards. We only perform IgG and Poly DAT's. Edited April 19, 2019 by ANORRIS added Link to comment Share on other sites More sharing options...
David Saikin Posted April 19, 2019 Share Posted April 19, 2019 On 02/21/2019 at 6:53 AM, Malcolm Needs said: Yes, but the card is European. I have no idea if it is available in the USA. I love this card. Had the opportunity to try it (thanks Malcolm). Too bad it's not available in the USA. I do not usually run a control with my IgG cards. When I test w anti-C3b,-C3d I use my A2 cell as a negative control/C3 sensitized cells as positive. Malcolm Needs 1 Link to comment Share on other sites More sharing options...
gene20354 Posted April 30, 2019 Share Posted April 30, 2019 We run the monoclonal control card in parallel with the poly card to catch the false positives. TRabs10 1 Link to comment Share on other sites More sharing options...
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