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galvania

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galvania last won the day on July 6

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About galvania

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    Seasoned poster
  • Birthday 05/09/1955

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    Female
  • Interests
    Jazz, birdwatching, gym
  • Location
    Castelletto di Vernasca, Italy
  • Occupation
    ex teacher/BMS in transfusion science. Now retired

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  1. Do keep us updated. I am sure we are all looking forward to news of a healthy baby
  2. Can I just point out here that no one serological test, or even combination of tests will detect all weak / variant Ds . And that includes women who test D+ but actually have a partial D and may make anti-D antibodies. It is SO important to know your reagents, and know what your anti-D reagents will and will not detect
  3. Sorry all, I replied on the other channel before reading all the above. ……...
  4. First of all, please do not worry. IF you do have anti-D antibodies, and they are real antibodies, this is only one of a number of tests that the doctors will do during your pregnancy to make sure everything is going OK with your baby. What they should do is recheck your blood for anti-D levels now and again in about 4 weeks' time to see if there is any change. It will show anti-D because of the Rhogam, but the important thing is to see whether the level increases significantly over time. Also, even now, if it is very high (VERY unlikely) then that would indicate it's real anti-D as opposed to the Rhogam. Ultrasound is a good idea. It is usually done anyway during pregnancy, but it will also show if something is happening that they need to react to.
  5. Apologies for not answering earlier. No, I did not mean 12 cells in the screening cell panel. I meant putting up 12 different antibody screens (12 different patients) each using a three-cell screening panel. Putting up = testing
  6. 1. did you think to do a DAT on the posttransfusion sample to see if the antibody was all 'stuck' on to the transfused red cells? 2. What method did you use to K-type the units of blood? did it involve an IAT? If so was the positive result a false positive because the unit had a positive DAT?
  7. sorry for the late reply. What I meant is that one tends to put up a batch of antibody screens. In the time taken to put up say 12 screens the cells can cool down enough for a cold anti-M in the plasma to latch on. On the other hand, panels tend to go up one at a time (1 patient at a time) so cells have less chance of cooling down. (Always assuming the screening cells and the pane are from the same manufacturer and being tested in the same method)
  8. In my case, temperature, as the screening cells and panel were both from the same manufacturer, therefore the same buffer system
  9. Because I've seen so many of them…………. But actually if your panel is not in the same buffer then pH or ionic strength could be the culprit rather than the temperature. Important to stress that these cold anti-Ms (in that they dont react strictly at 37°C) have no clinical significance. If it happens again, you can try the following: 1. Repeat the panel, incubating for 15mins at RT (on a Coombs card). The results will be stronger in this case. AND 2. Repeat the screen in the following way. Warm the cells to 37°C (best just to use a small aliquot - you dont want to 'cook' the whole bottle). And put the Coombs card (foil still on) in the incubator for 15mins. Put the patient's plasma in the incubator. IN the incubator, pipette 50ul of cells into the appropriate wells followed by the patient's plasma. Incubate for 15mins. At the same time, start the centrifuge empty. This warms the centrifuge up a bit. After 15mins put the incubated card into the centrifuge which will have now stopped and centrifuge immediately. This should negativise the screen results. This is about the nearest you can get to doing a gel test at strictly 37°C
  10. and what is wrong with this patient? what drugs is (s)he on?
  11. It depends a bit on how you work. If you are working manually then it is quite common to pipette a whole series of tests . In this time the cells can cool down enough for the anti-M to latch on, and once it's on it stays on. On the other hand, panels tend to go up individually so the cells stay warmer
  12. I'm no longer working but if this is an antibody then it sounds logical that it could cause non-sp results…..
  13. The DAT MIGHT have a considerable IgA component. Some anti-IgG (and hence also poly) will pick up IgA more than others regardless of method.
  14. might be worth checking to see if she actually has antibodies to IgA
  15. The reverse cells are normally pooled. So the lady's antibody is probably reacting with some of the cells in the pool and not others. Usually the Rh pheno is the same on all the cells in the pools so it probably is not due to the anti-c unless your provider does not ensure that they are all the same, or one of them is a c-variant. Could easily be an anti-M or Lea or Leb though
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