Jump to content


  • Content Count

  • Joined

  • Last visited

  • Days Won

  • Country


galvania last won the day on January 7

galvania had the most liked content!

About galvania

  • Rank
    Seasoned poster
  • Birthday 05/09/1955

Profile Information

  • Gender
  • Interests
    Jazz, birdwatching, gym
  • Location
    Castelletto di Vernasca, Italy
  • Occupation
    ex teacher/BMS in transfusion science. Now retired

Recent Profile Visitors

The recent visitors block is disabled and is not being shown to other users.

  1. Three to four a week out of how many tests? Where are you getting your cells from? Is the reaction strength the same with all cells? Are you working manually or with an automat? Have you noticed a correlation with these patients' ABO groups? Also do you have any lookback as to whether the babies of these women were jaundiced or anaemic post delivery? Or were they OK?
  2. That's awful Frenchie. So much for all the talk about our 'valued public service workers'.
  3. you pretty much have to be retired to have used it routinely. Like me. And Malcolm
  4. Do keep us updated. I am sure we are all looking forward to news of a healthy baby
  5. Can I just point out here that no one serological test, or even combination of tests will detect all weak / variant Ds . And that includes women who test D+ but actually have a partial D and may make anti-D antibodies. It is SO important to know your reagents, and know what your anti-D reagents will and will not detect
  6. Sorry all, I replied on the other channel before reading all the above. ……...
  7. First of all, please do not worry. IF you do have anti-D antibodies, and they are real antibodies, this is only one of a number of tests that the doctors will do during your pregnancy to make sure everything is going OK with your baby. What they should do is recheck your blood for anti-D levels now and again in about 4 weeks' time to see if there is any change. It will show anti-D because of the Rhogam, but the important thing is to see whether the level increases significantly over time. Also, even now, if it is very high (VERY unlikely) then that would indicate it's real anti-D as
  8. Apologies for not answering earlier. No, I did not mean 12 cells in the screening cell panel. I meant putting up 12 different antibody screens (12 different patients) each using a three-cell screening panel. Putting up = testing
  9. 1. did you think to do a DAT on the posttransfusion sample to see if the antibody was all 'stuck' on to the transfused red cells? 2. What method did you use to K-type the units of blood? did it involve an IAT? If so was the positive result a false positive because the unit had a positive DAT?
  10. sorry for the late reply. What I meant is that one tends to put up a batch of antibody screens. In the time taken to put up say 12 screens the cells can cool down enough for a cold anti-M in the plasma to latch on. On the other hand, panels tend to go up one at a time (1 patient at a time) so cells have less chance of cooling down. (Always assuming the screening cells and the pane are from the same manufacturer and being tested in the same method)
  11. In my case, temperature, as the screening cells and panel were both from the same manufacturer, therefore the same buffer system
  12. Because I've seen so many of them…………. But actually if your panel is not in the same buffer then pH or ionic strength could be the culprit rather than the temperature. Important to stress that these cold anti-Ms (in that they dont react strictly at 37°C) have no clinical significance. If it happens again, you can try the following: 1. Repeat the panel, incubating for 15mins at RT (on a Coombs card). The results will be stronger in this case. AND 2. Repeat the screen in the following way. Warm the cells to 37°C (best just to use a small aliquot - you dont want to '
  13. and what is wrong with this patient? what drugs is (s)he on?
  14. It depends a bit on how you work. If you are working manually then it is quite common to pipette a whole series of tests . In this time the cells can cool down enough for the anti-M to latch on, and once it's on it stays on. On the other hand, panels tend to go up individually so the cells stay warmer
  15. I'm no longer working but if this is an antibody then it sounds logical that it could cause non-sp results…..
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.