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galvania

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galvania last won the day on April 12

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About galvania

  • Rank
    Seasoned poster
  • Birthday 05/09/1955

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  • Interests
    Jazz, birdwatching, gym
  • Location
    Fribourg, Switzerland
  • Occupation
    teacher/BMS in transfusion science

Display Name History

  1. I quite fancy an expedition to Loch Ness - with or without the monster......
  2. Sorry - just seen this is UK. Do you have access to DC screening cards with anti-IgG, -IGM, and IgA?? (Sorry colleagues from the US - this will mean nothing to you)
  3. Yes - however, I would have expected an increase in the reaction with the R2R2 cell in the enzyme-IAT and it did not move. Or did it go from a weak 1+ to a strong 1+. Even so - for a 1+ in IAT, I would expect a +++ in an enz-IAT for a 'normal' anti-D. Which country is this in?
  4. Interesting. So, a qualified lab scientist can take on complicated nursing activities too......? OK - so who shall I practice on? any volunteers????????
  5. I think doing enz-IAT is missing the point here. Usually one would expect an anti-D to react more strongly or at least AS strongly with papain-treated cells (2-step) than with non-treated cells in an IAT. Of course there are the odd exceptions and this is clearly one of them. So, provided your weak anti-D control is working correctly with the papainised cells, then it has to be patient-related rather than instrument/reagent related. I know it's a bit weird but I wonder if this anti-D is a mix of IgA and IgG and that sometimes the IgG component is not visible, and that the IgA part only reacts by IAT but not in papain. Having said that , I don't KNOW that an IgA antibody would not react in pap. I'm just surmising. Malcolm, do you know whether IgA antibodies would react in pap?
  6. Sorry Malcolm. Mea Culpa. Can I plead old age and being very tired?
  7. I don't suppose there is any possibility of seeing the images in the gel cards is there?
  8. So how strong is the reaction in Coombs?
  9. Are you using a one-step or a two-step enzyme technique?
  10. What exactly do YOU mean by traceability in this context, Dahboud?
  11. It depends what type of population you are testing. If you are testing patients, then ideally you need to have those antigens where antibodies may only react against homozygotes present in a double dose. It is almost impossible to do that with just two cells - regardless of whether you are testing in gel or in tube. So, three cells for patients. Two cells is fine for donors where very weak antibodies are less important
  12. It is also important to check whether this patient has EVER been pregnant or had a transfusion.
  13. One of the units could have had a weak K antigen (depression due to other antigens present in the KEL system) which was missed (through no fault of anybody) and called K-
  14. What needs to happen now is a thorough investigation of WHY this issue happened - not to apportion blame, but to ensure that it can never happen again due to an error in the lab
  15. It depends what you have available. Here there are 'Coombs' cards (either poly or just IgG) for doing the DAT; most of the time a positive result would then be tested on a card that differentiates between IgG and complement or IgG, IgA, IgM and complement. Both of these two cards have a control on them which is the equivalent to your 'saline control'. I too have seen some lovely cold agglutinin disease samples that are positive across the board, saline control included