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galvania

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galvania last won the day on June 4

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About galvania

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  • Birthday 05/09/1955

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    Jazz, birdwatching, gym
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    Castelletto di Vernasca, Italy
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    ex teacher/BMS in transfusion science. Now retired

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  1. The DAT MIGHT have a considerable IgA component. Some anti-IgG (and hence also poly) will pick up IgA more than others regardless of method.
  2. might be worth checking to see if she actually has antibodies to IgA
  3. The reverse cells are normally pooled. So the lady's antibody is probably reacting with some of the cells in the pool and not others. Usually the Rh pheno is the same on all the cells in the pools so it probably is not due to the anti-c unless your provider does not ensure that they are all the same, or one of them is a c-variant. Could easily be an anti-M or Lea or Leb though
  4. Also, the polyclonal (human) reagents will give false pos results in samples with pos DATS. But anyway, finding sufficiently good human antibodies to manufacture reagents from is getting harder and harder. So definitely monoclonal
  5. Are you working in gel? If so, repeat in tube. Tube is more sensitive for weak reverse reactions
  6. This is well known and there are indeed other threads concerning this. Transfused cells may have different densities to the patient's own cells and therefore when you spin the tubes in the centrifuge you may find that the transfused cells and the patient's cells separate. Then, depending on where you sample from in the tube, you will either get only transfused cells, only patients cells or a mixture, giving a mixed field. Typically instruments will select cells from the bottom of the tube, whereas pipetting by hand, one normally samples from the top, giving a discrepancy between the two methods.
  7. what are the antibodies and what is the reason for needing the titration?
  8. If you are seeing a lot of weak results in your anti-D well that subsequently turn out to be negative, I suspect the reason to be one of manipulation rather than anything serological. I would guess that NONE of these babies are group O. I am guessing that you are seeing carryover from your anti-AB. It can happen that if cards are stored somewhere where condensation can take place then drops of antiserum can condense into the reaction chamber and 'jump' into the next well or even next 2 wells when you remove the aluminium. This can cause false positive results. I suggest you check the cards before pipetting in them and see if there are any signs of these drops. Don't use the cards if there are and look for another place to store them
  9. what is the baby's DAT? Is it anaemic? Jaundiced?
  10. Beware of using reagent antisera to do your QC. The buffers in the reagents can interact with the buffers in the cells and, if using anything other than tube, the buffers in the support medium. You may get false positive results
  11. IF it looks like a real reaction and it's only one cell then it may well be an antibody against an LFA. If you send a good sample from the patient together with the cell concerned to your reference centre, they may be able to identify it for you - although that's on an interest-only basis
  12. Oh I quite agree Malcolm - just that those two possibilities need to be taken into account with this type of reaction
  13. or it might have been anti-buffer or cold 'rubbish'......
  14. Cliff - I cant see where to edit my e-mail address. It is now <removed by admin>. And no - I have no problem that anyone in the forum can see this
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