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galvania

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galvania last won the day on July 23

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About galvania

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    Seasoned poster
  • Birthday 05/09/1955

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    Jazz, birdwatching, gym
  • Location
    Fribourg, Switzerland
  • Occupation
    teacher/BMS in transfusion science

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  1. Hi Galvania,

    Do you have more information about cases of difficulty in adsorbing out allo-anti-Jra ?

    We have tried 6X adsorption still failed. Its reactivity like a HTLA.

     

     

    Emergency room

     

    1. galvania

      galvania

      unfortunately no more than the comment that I posted - that our reference lab reports difficulty too

      sorry

       

  2. galvania

    Anti-Jra in pregnant mother

    Anti Jra is quite common in Korea and they sometimes send some samples to our reference lab in Switzerland for testing. The reference lab have previously reported difficulty in adsorbing out allo-anti-Jra too
  3. It looks to me as though you may have some spontaneously agglutinating IgM in the sample. Do you have some neutral cards? If so, put 50ul of the cell suspension on the neutral card and centrifuge directly. That won't necessarily differentiate between an agglutinating IgG and an IgM but it would tend towards the latter if it were positive. Also, in case it is LISS-enhanced, you could try suspending your cells in Cell Stab or Alsevers or PBS and repeating. Just beware of cells 'training' through the gel. And make absolutely sure that whatever you use has well come to room temperature before you use it! Anyway, from those results, even though the control is positive, you clearly have a major component of IgG present.
  4. In my experience good monoclonal anti-D reagents will pick up all but the weakest of D antigen variants (by which I mean all normal D+ and all D variants with a reasonable number of D antigens on their surface). The positive DAT will not interfere with them unless it's caused by clinically significant cold haemagglutinin disease. So only the very very weak D variants will come up anyway with the weak D test in an IAT but not in direct testing. So if you did not do the weak D testing you might miss a few cases. Those few would have to nonetheless be able to stimulate mum into making an anti-D. I would be very interested to know exactly how many cases you see where the routine testing (using a sensitive method and good quality monoclonal anti-D reagents) is negative and the Weak D testing was positive (with a negative DAT). I wonder if it's more or less than the number of D variant mums you miss because they group as normal D+ ........... Also, for the anti-D to give you a false negative result because all the antigen binding sites are already loaded, your DAT would have to be VERY strong - and mum would have a known (hopefully), very high titre, antibody. So you would have to carry out an eluate anyway on baby's cells and find for example that mum has a high-titre anti-D and you can elute anti-D from the baby's apparently D-negative red cells. That's when you know that baby really is D+ and all the D sites are saturated. I've seen this twice in 'real life'.
  5. galvania

    Group O platelets titer

    the trouble with titres is that the result is method dependent - so unless the methods are identical, comparing across labs is not much use
  6. You are talking about patients? The biggest problem as I see it is that you will get a false positive result due to the positive DAT and then transfuse with D+ blood. Either you go to a lot of time and effort and strip off the antibodies from the red cells as Malcolm said - or, probably a lot easier - and safer - treat them as D-negative
  7. You will not know just on the basis of this single test. Looking under the microscope can help (not at the card!) to see if rouleaux is present. The diagnosis can also help. Also a cold auto-antibody will disappear if the test is carried out strictly at 37°C; the rouleaux will not.
  8. Cold auto antibodies can give you false positives in the forward group - but will also give you a false positive in the control well, so this will be picked up. The reverse group will also be positive. I have yet to see a case of 'normal' rouleaux that is so heavy that it will do this, but it is possible if bloods are taken directly after administration of plasma expanders.
  9. galvania

    Blood group discrepancy Ortho vision analyzer

    The manual result is no more accurate than the result obtained on the analyser. Personally I would report as unable to interpret due to mixed field post transfusion
  10. galvania

    Blood group discrepancy Ortho vision analyzer

    Would you call that a limitation of the automat? Personally I would not - any more than saying that sampling from the top of the tube is a limitation of manual sampling. It implies that there is a problem and it lies with the automat - when in fact it's a simple manifestation of a biological property of red cells
  11. galvania

    Gold Medal.

    Much deserved. I allocated you a winners cup. The icon says'thanks' but read the icon more as much deserved congratulations One of my colleagues will be in Brighton. I'll get him to come up and shake your hand (and maybe buy you a beer....) anna
  12. galvania

    Blood group discrepancy Ortho vision analyzer

    Likewise. This is very common and can occur on any automats
  13. galvania

    Mycoplasma pneumoniae

    And going back to the first case. Were those results 'made up' or did you actually have a patient that gave those results?
  14. galvania

    Polyagglutination

    sounds a bit too much like alchemy to me........but I don't pretend to be a chemist
  15. galvania

    Mycoplasma pneumoniae

    Tabbie - what exactly is behind this question? I get the impression there is something you are not telling us??????
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