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galvania last won the day on July 23

galvania had the most liked content!

About galvania

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    Seasoned poster
  • Birthday 05/09/1955

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    Jazz, birdwatching, gym
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    Fribourg, Switzerland
  • Occupation
    teacher/BMS in transfusion science

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  1. You will not know just on the basis of this single test. Looking under the microscope can help (not at the card!) to see if rouleaux is present. The diagnosis can also help. Also a cold auto-antibody will disappear if the test is carried out strictly at 37°C; the rouleaux will not.
  2. Cold auto antibodies can give you false positives in the forward group - but will also give you a false positive in the control well, so this will be picked up. The reverse group will also be positive. I have yet to see a case of 'normal' rouleaux that is so heavy that it will do this, but it is possible if bloods are taken directly after administration of plasma expanders.
  3. galvania

    Blood group discrepancy Ortho vision analyzer

    The manual result is no more accurate than the result obtained on the analyser. Personally I would report as unable to interpret due to mixed field post transfusion
  4. galvania

    Blood group discrepancy Ortho vision analyzer

    Would you call that a limitation of the automat? Personally I would not - any more than saying that sampling from the top of the tube is a limitation of manual sampling. It implies that there is a problem and it lies with the automat - when in fact it's a simple manifestation of a biological property of red cells
  5. galvania

    Gold Medal.

    Much deserved. I allocated you a winners cup. The icon says'thanks' but read the icon more as much deserved congratulations One of my colleagues will be in Brighton. I'll get him to come up and shake your hand (and maybe buy you a beer....) anna
  6. galvania

    Blood group discrepancy Ortho vision analyzer

    Likewise. This is very common and can occur on any automats
  7. galvania

    Mycoplasma pneumoniae

    And going back to the first case. Were those results 'made up' or did you actually have a patient that gave those results?
  8. galvania


    sounds a bit too much like alchemy to me........but I don't pretend to be a chemist
  9. galvania

    Mycoplasma pneumoniae

    Tabbie - what exactly is behind this question? I get the impression there is something you are not telling us??????
  10. galvania

    DVI +ve or DVI -ve?

    Very simply - NO, NO and NO. It is not for nothing that it is called ....'for donors'. However, just so something else is well understood. Using a card that is 'negative for DVI' does not mean it will be negative for any other Partial D.
  11. galvania

    Auto adsorption

    If the genotype was done with full sequencing and the phenotype was tested with various clones and all of that said negative, how did the paper come to the conclusion that this was an auto anti-C rather than an allo-anti-C?
  12. galvania

    Mycoplasma pneumoniae

    And I forgot to say - no, this is not the pattern of an anti-I
  13. galvania

    Mycoplasma pneumoniae

    Presuming you are in a normal lab, you should 1. Find out about transfusion history as a matter of considerable urgency, including plasma products 2. do your normal exclusions (I would be a bit worried about an anti-Jkb with something else 3. Use additional cells 4. Put up a saline RT panel 5. Fully phenotype your patient 6. Do a DAT - look very hard for a mixed field Then review!
  14. galvania

    Lewis exclusions

    the same as you would for any other antibody But you need to make sure that if there is an anti-Lea or -Leb present that it is reacting strictly at 37°C. Otherwise no clinical relevance at all
  15. Cells are coated with complement artificially. some methods will also cause IgG to be coated. IF the cells are used according to the method they were designed for the manufacturer will have made sure that , in that method, the IgG does not react. The problem starts when the complement-coated cells are used in other methods or, worse, for the CAP survey. The C3-coated cells that are used for the CAP survey almost always react with anti-IgG in gel because of this - but the cells used are actually designed only to be used with anti-C3 in tubes. so my advice would be, if you are using complement coated cells to control your anti-complement reagents, don't use them to check for negative reactions in anti-IgG

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