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galvania

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galvania last won the day on October 28

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About galvania

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  • Birthday 05/09/1955

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    Fribourg, Switzerland
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    teacher/BMS in transfusion science
  1. wAIHA with IgM and C3c/C3d coating

    OK Malcolm, in that case you can exclude it. I hadn't noticed the little English flag on the first post! Has anyone thought to do an eluate? That might be helpful
  2. B(A) Phenotype?

    Or, more mundanely, the reaction in the anti-A could be down to carry over. When working with gel, if there is condensation in the reaction chamber, or even worse, directly under the aluminium, this can be carried over when pipetting or when removing the aluminium. As the antiserum is so strong this can lead to false positive reactions - rarely a 4+. Is it too late to see a picture of the pipetted card?
  3. Lewis A

    Malcolm, are you really trying to kill Mr. Lewis??????????
  4. wAIHA with IgM and C3c/C3d coating

    going back to the AIHA, I wonder if there's a warm IgA component in there?
  5. Rh D discrepancies with method changes

    And I would like to stress one thing. There is NO serological method that will allow you to detect ALL partial and weak Ds. Variant Ds come in all shapes and sizes. There are some weak Ds that have so few D antigens that the most sensitive 'normal' serological techniques will not pick them up - they will be typed as D- (including donors). On the other hand, some partial Ds have a sufficiently high number of D antigen sites that you will detect them as a normal D+ (even patients). And there are some Partial Ds that react with all commercially available Anti-D clones. So you will pull these up too as D+ (even for patients). The message behind this is - You WILL misgroup some D variant patients as D+ and you WILL misgroup some D variant donors as D-. And you have been misgrouping them for ever. Until (and if) genotyping becomes as easy, fast and cheap to do for every routine group, then you have to learn to live with it!
  6. Daily Reagent QC requirements

    Oh dear, whatever happened to common sense? If you are testing an antibody screen, for example, why do you need a negative control? The majority of your samples will give a negative result. So you know that the negatives are working. But you DO need a positive control to make sure that the reagents are working. similarly, if you have an anti-k reagent, where are you going to get a k-neg cell to use as a control each time (in a normal lab)? For ABO, if you have an A, a B and an O you have covered everything, even the reverse group, as with the A and the B one of the reverse group cells will be negative each time.....
  7. I know some labs who run an AC with their screen, AND with their panel, AND with their XM - which they do even if their screen was negative AND get very worked up if the results are discrepant - like between a negative result and a trace reaction that's there if you look through a magnifying glass. Personally I would only do an AC with my panel if everything is coming up pos.
  8. You're STILL in that era. Genetics is discovering new things every day - not just about blood grouping either
  9. Sorry - who is talking about warm sand? But whatever - everything has to be 'validated' nowadays. Regardless if it makes any sense or not
  10. We used to do the same. I remember we had one patient whose cold aggs were so strong and had such a high thermal range we had to wheel her into our 'warm room' (a small room where we used to incubate all our microbiology stuff) to be bled for any tests. And we had to bring small bits of equipment into the room as well. But then I'm going back a very long time and tests were very unsophisticated then compared to now. Can't quite see that happening today, somehow
  11. Rh D discrepancies with method changes

    Can you hear me groaning ?
  12. Polyagglutination

    Weird.....Why did they order the lectin work up anyway? doesn't really look like a polyagglutinable sample from those results - but i don't have very much experience with lectins I think I would be inclined to do an antibody screen in the cold (thinking auto-anti-I or -i)
  13. Rh D discrepancies with method changes

    But look on the positive side. If you are seeing a discrepancy, this should alert you to the presence of a variant that you might not have known was there otherwise
  14. antibody screen on cord blood

    Why do I get the distinct feeling that someone is not giving you the full picture on this one....???
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