RichU

Our irradiated red cells have an updated expiry of 14 days max and are not irradiated if older than 2 weeks. Non-Hodgkins lymphoma does not require irradiated products.

We have a policy where we do not perform a cross-match on the first sample of a patient. This sample gets a full group card (forward and reverse group) and a confirm ABD card. The next sample, and any future samples, only have a confirmation (forward group). These 2 samples must be bled at least 10 minutes apart so that there is less chance that the incorrect patient is bled twice. Our blood donors get the full group card and a donor ABD card (Dvi+) on first donation. Further donations just get the donor ABD card

Thanks for your speedy reply Malcolm. To clarify - If a R1wR1 cell is positive by IAT in the screen/panel but negative against (the same) enzyme treated cells, can I exclude Anti-D, anti-C, anti-e and anti-Cw? Regards, Rich

Hi all, I am happy to use a negative enzyme result to exclude Rh antibodies. However, I have a nagging doubt about being able to do this with anti-Cw but can't find any reference to this. Have I made this up or can anyone point me to where I can find this in black and white. Cheers, Rich

Serious Hazards Of Transfusion (SHOT) have haemolytic transfusion reactions in separate reporting categories. All of the following can be 'Serious Adverse Reactions'; HTR Acute HTR Delayed HTR Hyperhaemolysis FAHR See SHOT definitions if you want more info. SHOT Definitions - Serious Hazards of Transfusion

@Malcolm Needs, I have done as you suggested and await a reply. Rich

Why are haemolytic reactions excluded from definition of ATR? SHOT in UK only defines Febrile, Allergic and Hypotensive Cheers

Hi Saeeed, I'm just curious to know where the auto control is. I would expect it to be in well 12 of the IAT panel card. Also, where are the enzyme panel results? Also, also, the group card seems to have a different number to the IAT cards - 476258 and 470258?

Hi and thanks for your replies. Sorry for not updating - i've been off work. Our enzyme method is papainised cells tested using BioRad NaCl cards. A follow-up sample reacted exactly the same for us but the reference lab report a unspecified antibody by IAT and enzyme IAT, don't say they cannot exclude anti-D or anti-E and require no further samples this pregnancy. Rich

Hi all, We have a 28/40 pregnant patient, group O neg with an antibody that fits exactly with anti-D on our IAT panel but is negative in enzyme. Previous sample reported by our reference lab as having non-specified antibody. Anti-D (and anti-E) could not be fully excluded. No record of prophylaxis. Any ideas? Cheers, Rich

I just answered this question. My Score PASS  

If the patient has had a recent transfusion, elution studies might show if the antibody coating the cells in the patient's sample has a specificity. Red cells with the corresponding antigen should be avoided unless you have previously typed the patient and can say whether it's allo or auto.

I just answered this question. My Score FAIL  

Ah ok. Makes sense. Cheers

How do you know a positive screen isn't caused by an alloantibody underlying the prophylactic anti-D unless you do an ABID?