gagpinks

Thanks everyone Clinician hasn't suspected transfusions reaction and hasn't asked for any investigations. This is something we noticed the results when they requested further blood unit. just wanted to know causes of DAT to be positive in C3d therefore shared on discussions board.

Hi we had patient who has known anti E antibody. He has been transfused with 2 units E- K- by IAT xmatch. 2 weeks iater we received sample for G/S and request for 1 unit of blood due to low Hb. However clinician haven't suspected any transfusions reaction. Performed antibody screen and found to be positive with Anti-E and Jka with DAT positive in IgG 1+ and C3d 2+. Would this be a transfusion reaction? Or patient had developed new antibody due to recent transfusions? What is reason for DAT to be positive in C3d as well? I think IgG antibody causes extracascular haemolysis . Is it due to antibody develop recently that might be IgM in nature ? Thanks in advance

Hi Malcom Could it be Matuhasi-Ogata phenomenon?

Is this mean autoantiboody absorbed better with Rh antigen? I am also trying to understand how RCI deal with pan-reactivity especially with HFA

Thanks Malcolm I just wanted be assured. Our SOP suggest it is good laboratory practice to get new sample after 2 units of platelet transfusions however i don't see any benifits if patient need regular platelet transfusions especially haem patients

Hi All If patient has 2 known historical blood group, do we still need valid group and save at time of platelet transfusion? I am trying to find out in guidelines but unable to find. If patient is on regular platelet transfusion how often should we repeat group and save and why?

Hi We perform DAT using polyspecific AHG card which detect IgG and/or C3d. If DAT is positive then we use monospecific card which detect DAT is positive due to IgG or c3d or both. This card also has control. Control must be negative.

You are well deserved for this.

I just answered this question. My Score PASS  

we did try this but apparently, it works very weekly than expected.

As Bankergirl mentioned you can use IAT method to confirm. But it will not differentiate between partial D and weak D

Hi I was reading articles on social media regarding 2-year-old girl developed anti-Inb ( In a news article from the USA). She has neuroblastoma and difficulty finding Inb negative blood. If a patient is on chemotherapy and required regular transfusion it is hard to provide blood in these situations. How severe anti-Inb can cause transfusion reactions especially when a patient is on chemotherapy? Can she have ABO and D compatible blood with methylprednisolone?

Reviewing this process so just wondering did you use any control with this process? Do we need to run control ? Can't find in guidelines

This could be due to anti c IgM in nature. I have seen this many time especially in pregnant lady.

Do you run any control with this process ? With IAT crossmatch we use OR1r cell with weak antiD.