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That is what my former supervisor used to say (he was a tech for over 50 years)! Get the titer up where you can work with it! God rest him!

I agree with Malcolm. In theory, there may be examples of anti-K that only react with K+k- cells, but in practice it's a very rare event. One of my former colleagues/mentors once said that one shouldn't worry about missing a weak antibody. If the patient were unfortunate to be transfused antigen-positive blood, the former weak antibody would be super-strong next time around !!! Problem solved.

I agree entirely with exlimey, except to say that even today's monoclonal antibodies need a potentiator. Many of them include a small amount of bovine albumin in the reagent bottle. I don't, however, agree with you Jermin. The reason being is that there is no such thing as a silly or daft question. The only silly or daft question is the one you (anyone) don't ask, because, if that question is not asked. the person who doesn't know the answer will never know the answer. Sadly, there are numerous examples of silly or daft answers!

The Rh typing reagents are designed to react that way. These days, the reagents are monoclonal, IgM in nature and give direct agglutination in a very short amount of time (similar to anti-A and other ABO reagents). Centrifugation is also usually part of the process. Antibodies to Rh antigens in patients (or donors) are typically IgG and require incubation and an antiglobulin phase. Most manual tube testing systems these days also use a potentiator to enhance reactivity and/or reduce incubation times. In the "bad-old-days", Rh typing reagents were human-source, IgG in nature and usually required incubation and an antiglobulin phase.

I like that ! None of this wishy-washy, barely reactive stuff.

I have an idea I may know who that was!

I have seen dosage a couple of times and a >K reacting at room temp only. I agree with Malcolm and his reasoning why K+k- cell is not required to rule out >K.

In my opinion (and that of the BCSH Guidelines) you do not need a K+k- red cell to rule out anti-K. If you look at the antigen profile of the red cells you use every day as screening cells, they will not have a K+k- cell, and yet you are ruling out the presence of anti-K (and any other antibodies directed against the major blood group antigens) with each sample that gives negative reactions with these red cells. In addition, if you look at the screening cell profile that the BCSH Guidelines recommend, they say that the K antigen MUST be represented, but NOT that these cells must be K+k-.

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I have seen about two cases of this in 43 years in blood transfusion. Yes, they exist - but so does Rhnull!!!!!!!!

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With ProVue's phasing out, there are likely some of you out there assessing what to get for replacement (Vision; switch to Immucor Solid Phase.....or, Grifols Erytra). We just purchased (and are now validating) the Erytra and I really like it (and NO, I promise I do not work for them and have no vested interest here). If anyone would like to know of our experience thus far, would be happy to share. NOTE: We previously used ProVue's (and I have used Solid Phase elsewhere). Thanks Brenda Hutson, MT(ASCP)SBB

I just answered this question. My Score PASS

I just answered this question. My Score PASS

Good to know! thanks.

We are going to RFP this coming year for some new automated analyzers. We did a quick look this spring at what is out there. I am surprised no one is speaking about the Biorad analyzers: newly released IH 500, IH1000 (Gel based) and Tango Infinity (4th generation solid phase analyzer) along with stand alone Gel readers and centrifuges. Although we are not sure which direction we are heading, I think they are certainly worth mentioning.