Amra23

Very useful information,thanks to everyone responded here!

Thank you so much,Malcom!

Thank you,Malcom!I'm finally enlightened !

Malcom,I would love to hear your ideas,please! My first option would be ccDEeK- if cross-matching is OK. Also I should say that the old unit of O ccDEEK- is not leucodepleted. But we do have leucoreduction filters. And about old units given to a new born..,is the high potassium levels the only concern?

I wonder why they still manufacture human reagents if that's the case...

Emergency case of a cardiac malformation on a new born baby with 0+ ccDEEK- DAT=neg, mother 0+ CcDEeK- . 0+ ccDEEK- fresh blood not available.Only 10 days old blood with this phenotype. Which is the best option for transfusion for this baby (it will be needed for the cardiac extracorporeal circulation),10 days old blood with his phenotype or fresh blood (within 5 days) with a slightly different phenotype?If the second option is the one,which phenotype would be best in this case? This kind of patients tend to receive multiple transfusions after surgery.

I didn't know that! This is something I will always have to remember!

Silly question...,but I'd really like to know,what is the difference in choosing between human and monoclonal reagents for ABO/D testing? Is it a first choice from these two?Is it the price? I work with Bio-Rad reagents and I know they have cards with human reagents but also identical cards with monoclonal reagents.Our lab use only monoclonal ones.

I work in cardiac surgery unit.It is very simple for us.We have schedules with the patients every day and we know every case in particular.Every unit of blood is labeled with the pacient's ID,ABO/Rh group,crossmatch number,the name of lab worker who performed the crossmatch and also the date of the test. We have 3 OR's and every time they need units of blood they call in our unit(which is only 2 floor distance) and we transport the units to them.They perform Bedside ABO/D test before every transfusion!All the dates from the blood unit are transcripted in patient's chart manually (we do not have a computer scan for that). So far everything works great. I am from east Europe country.

Thank you,Malcom, your answer was very enlightening for me! Have a great day!

Hi all. We have an 11 months old female pacient O Rh(D)negative Ccddeek- on forward group.Reverse group shows no antibodies in B cells and +3 with A1 cells.Controls negative.I performed a weak D test and after IAT faze I got a +3 result with anti-D(monoclonal).So I believe is a O Rh( D)weak type 1,right? Can we give her red blood cells D positive with no problem?Or we'd be much safer with D negative transfusion? Thank you.

Thank you,Malcom,your answer was very useful to me!I have so much to learn from you!

We had an 65 years old male pacient with A2B Rh(D)+ blood type.Never transfused before,antibodies screening negative.We asked our blood bank transfusion guidelines for this pacient in case of need(he was hospitalised for heart surgery).We got the answer that we can give him AB Rh(D)+ regular red cells (the chances he would develop A1 antibodies is 20 percents as far as I know) and only later,if he develops that antibodies we should switch him on B or O.What do you think about this?Is this correct in your transfusion policy? (Lucky pacient didn't need transfusion of red cells after all,only FFP) Just to satisfy my curiosity...and learn,of course.

We decided to prepare Rh(D) negative red cells for this pacient. We have a very strict protocol in our hospital.We perform ABO and Rh typing,Kell and Rh phenotype when needed,DAT and salin/enzyme/IAT for compatibility test and we request blood bank help only when discrepancies are descovered. Thank you so much for your answer,Malcom!

The pacient will be programmed for a cardiac by-pass(miocardial ischemia);he has also diabetes type 2 and mixed dyslipedimia.Of course he is on medication for all theese. We did not request the blood bank to perform tests for anti-Fya and anti-Fyb, and/or anti-Jka and anti-Jkb.