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exlimey last won the day on November 2

exlimey had the most liked content!

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About exlimey

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    Gaithersburg, MD, USA
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    IRL; Reagent Manufacturing

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  1. I just answered this question. My Score PASS  
  2. BloodBankTalk:Allergic Reaction

    I just answered this question. My Score PASS  
  3. Phenotying

    The Rh typing reagents are designed to react that way. These days, the reagents are monoclonal, IgM in nature and give direct agglutination in a very short amount of time (similar to anti-A and other ABO reagents). Centrifugation is also usually part of the process. Antibodies to Rh antigens in patients (or donors) are typically IgG and require incubation and an antiglobulin phase. Most manual tube testing systems these days also use a potentiator to enhance reactivity and/or reduce incubation times. In the "bad-old-days", Rh typing reagents were human-source, IgG in nature and usually required incubation and an antiglobulin phase.
  4. Rule out Anti-K

    I like that ! None of this wishy-washy, barely reactive stuff.
  5. Rule out Anti-K

    I agree with Malcolm. In theory, there may be examples of anti-K that only react with K+k- cells, but in practice it's a very rare event. One of my former colleagues/mentors once said that one shouldn't worry about missing a weak antibody. If the patient were unfortunate to be transfused antigen-positive blood, the former weak antibody would be super-strong next time around !!! Problem solved.
  6. I just answered this question. My Score FAIL  
  7. It sounds as if the "real", actionable result is the 24-hour reading - this should be recorded in the medical files. The "quick-and-dirty" initial test, while not very sensitive, may still be useful in some cases of extreme contamination. It may give the physicians a leg up on treatment. Perhaps a two-field record could be designed? Test #1 = Immediate; Test #2: 24-hr. The interpretation algorithm would include both results.
  8. 2rd determination of recipient's ABO

    It's probably all tangled-up in training, competency and proficiency. Maybe an administrative nightmare?
  9. Pregnancy and phenotyping?

    Yeah, I know. I was just being silly, pointing out that sometimes what sounds like a reasonable idea is often impractical and mostly useless. Proposed new policy: Type them once every week for the duration.
  10. Pregnancy and phenotyping?

    Very good point. One could argue that ALL pregnant women should be phenotyped specifically to look for mixed fields.
  11. Pregnancy and phenotyping?

    I'm assuming....yes, that gets me into trouble all the time......that you're worried about antigen suppression in pregnant women? Antisera licensed in the USA should have been tested extensively with samples from such patients. If they were unable to correctly phenotype samples from pregnant women, it's unlikely that they would have been approved. As Malcolm points out, the only real troublemakers are the Lewis antigens.
  12. Eek ! I don't like the sound of that. Ethylene oxide (ETO) is a gas used to sterilize materials that can't tolerate other processes (heat/radiation).
  13. A1 Lectin Proficiency Testing

    I'm certainly not a regulatory expert, but if it is indeed a requirement, I would think that a general "other antigen typing" proficiency would cover you. I can't imagine why one would need proficiency testing for one, or each, typing antiserum.