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exlimey

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exlimey last won the day on October 23 2023

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    Gaithersburg, MD, USA
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    IRL; Reagent Manufacturing

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  1. I just answered this question. My Score PASS  
  2. Not she, but it's possible the person that conveyed it to me got it from Dr. Worlledge.
  3. Something one of my mentors said early in my career: "Don't worry about junk. If it's a real antibody and you transfuse against it, it'll be nice and strong by the next time you see the patient."
  4. Your comments are spot-on, Malcolm. A "reference range" is typically the expected result from normal individuals. This is fine for something like a platelet count or a creatinine quantitation (or any of the chemistries), but is absolute nonsense for an assay that has only two potential outcomes: Positive or Negative. I suppose "Indeterminant" might be a third option. Here's your Reference Range for Antibody Screens: Positive / Negative / Indeterminant
  5. Concur with the above. It would have be a very serious F-M bleed to impact phenotyping. While there is a theoretical risk of mixed field, and potentially spurious interpretation of the results, if a gravid patient develops an antibody that late in the gestation, the very small risk of mistyping/reporting is worth taking.
  6. Is this approach applied to rare units ? For example, a donor who is Kp(b-), with anti-Kpb in their circulation. Granted, such a unit would probably only go to someone who already had anti-Kpb, so technically there would be no impact (analogous to Mabel's anti-D scenario). Just curious.
  7. It's remotely possible that Ortho use different diluents for different cells. I remember a rumor that "fresh" cells are suspended in one kind of diluent, but frozen-thawed cells are in a similar diluent with a couple of extra chemicals to compensate for the freeze-thaw process. I think that frozen-thawed (deglycerolized) cells are sometimes used in emergencies when the scheduled donor(s) don't appear.
  8. I agree with all the previous comments. You cannot manage a transfusion reaction in a patient who has died from lack of blood. One thing to add: In the time before time......emergency release units were always O negs. However, today's practice has evolved in a risk-based manner and it is now accepted that O pos units can fulfil this function. Perhaps ironically, if the old practice had been employed in this case (use O negs), it would have been very unlikely that this patient would get a E+ unit.
  9. Any of the major laboratory suppliers can help with lab design and furniture, for a price, of course. VWR (Avantor) and Thermo-Fisher are probably top of the list. I think that Mabel is referring to "Herman Miller" - a longtime standard for labs and offices. I think they may sell direct or through the distributors. Good luck. Designing a new space can be both fun and frustrating. Nothing can ever be perfect, compromises always have to be made.
  10. I think you can just use basic 40% glycerol (in water). Glycerol is available from many sources. Use an equal volume of 40% glycerol to washed, packed red cells. Freeze @ -80 C is small aliquots. I don't remember the details of recovery, but it involves washing with a couple of different Saline concentrations, akin to deglycerolizing red cells for transfusion. The "Valeri Method" - see the article below. Vox Sang 2000;79(3):168-74. An experiment with glycerol-frozen red blood cells stored at -80 degrees C for up to 37 years. C R Valeri 1, G Ragno, L E Pivacek, G P Cassidy, R Srey, M Hansson-Wicher, M E Leavy
  11. Moving seems to be very common after retirement, but It would be tough to leave Bend. Beautiful city and environs. I suggest fly fishing, but I'm biased. Lots of opportunities where you live, some of the more famous waters. Lots of mental stimulation, too (although not medical), with a heavy dose of Zen.
  12. Wow. That's bizarre. The routine retest of incoming materials detected the "transplanted" segments, only because of an Rh difference. If the transplants just happened to be the same ABO/Rh as the host - fairly good odds for an O+ to another O+, for example, it would be undetectable. I wonder how many times that's happened ? No way to know, I suppose.
  13. This is fascinating stuff. A lot of science, learned the very hard way, with a heavy dose of art. I don't envy those having to make these calls.
  14. Thanks. Probably an unanswerable question: How low a titer is "low enough" ? A follow-up.....can one transplant an A1 kidney into an A2 patient with anti-A1 ?
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