Hemo bioscience is currently developing a new product to be used for validation of blood bank instrumentation. To better understand the challenges associated with new method implementation, we would like to speak with 5-10 individuals who have recently, i.e. within the last year, implemented a new automated method for pretransfusion testing.
To be considered, please send an email to email@example.com with answers to the following:
Which instrument did you implement and when?
Which assays did you validate/qualify for your new instrument?
If selected to participate, a short phone call will be scheduled to further discuss your instrument validation/qualification process.
To show our appreciation, those who are selected and complete the project will receive an Amazon gift card via email.
Jimmy Lowery, MT(ASCP)SBBcm
Product Manager, Hemo bioscience
I searched the forum and read some discrepancy info from back in 2013 and 2014 but most of those patients had history of transfusion. Two days ago we had a prenatal T&S. No blood transfusions involved. We had no previous history of type or transfusions on file. The sample was tested on the ECHO and clearly typed as D negative with no discrepancies or concerns with both Anti-D reagents. Patient was reported as D negative. The same day we receive a call from the patient's midwife stating the patient has a history of being B Positive. We pulled the original sample and repeated testing on the ECHO. The repeat testing resulted in ? with at least one of the Anti-D reagents and resulted as NTD. (No type determined). We proceeded to perform tube as well as ECHO weak D testing. Patient was 2+ positive in tube with AHG phase using the exact same reagents used on the ECHO and 4+ positive with both Anti- D reagents ECHO weak D. My question is why did the initial test not react? If this was a particular variant etc., I could chalk it down to method or reagent but having one result on the same sample tested on the same day and then finding another result hours later when repeated does leave me wondering. Any ideas?? I will say the sample was EDTA and was refrigerated for several hours prior to the repeat testing.
Hi. We encountered an interesting case tonight. A male patient with no history of transfusion (also said he's never been transfused) typed beautifully A Pos. His screen, however, was positive 3+ for cells 1 and 2 and then D pos panel cells ranged from 1+ to 3+. The D negative panel was negative and antibody ID panel was positive for all D pos cells.
We then looked as his med list. He's on Bicalutamide, but there's no mention of BB interference in any literature I've Googled. He has a hx of prostate cancer and family hx of malignant neoplasms.
Tube results were negative across the board (I used both LISS and no LISS to see if any difference). They were text book, beautifully negative at that for all phases.
What could be an explanation of this??? Is there any cases of solid phase D reacting due to meds? Meds are the only conclusion we've come to so far. Any thoughts???