Desoki Posted September 19, 2012 Share Posted September 19, 2012 Dear friendswhen we should use albunim 6% as a control, and albumin 22% as control, and why? Link to comment Share on other sites More sharing options...
David Saikin Posted September 19, 2012 Share Posted September 19, 2012 I use 6-8% albumin for my Rh control; theoretically you only need to run it when the pt types as AB+. I would never use 22% alb as a control (unless using a high protein anti-D: has there been one available in the past 20 yrs?). Link to comment Share on other sites More sharing options...
galvania Posted September 19, 2012 Share Posted September 19, 2012 You need to use a control that is equivalent to the reagents you are controlling. Most of the time, companies sell a reagent control. This is actually NOT a waste of money. The reagents contain lots of things in their buffers in addition to albumin and you can occasionally get patients who react with these other things too, giving you a false positive result rrcc1974 and Abdulhameed Al-Attas 2 Link to comment Share on other sites More sharing options...
Justina Posted September 19, 2012 Share Posted September 19, 2012 We use 7% Albumin as control in patients that type ABpos (rule out polyagglutination). Link to comment Share on other sites More sharing options...
Mabel Adams Posted May 1, 2014 Share Posted May 1, 2014 We are implementing gel blood type testing at one of our hospitals and I am running into all sorts of opinions of how to make up 6% albumin to use as a negative control for the reverse typing test. We don't think they will be able to come by AB plasma consistently so we are looking at other options. What are others doing? Do you use 22% bovine albumin and dilute with saline, or MTS diluent 2 or MTS Diluent 2 Plus? Or do you use 25% human albumin and dilute with saline or...? Link to comment Share on other sites More sharing options...
Eagle Eye Posted May 2, 2014 Share Posted May 2, 2014 22% diluted to 6% with saline Link to comment Share on other sites More sharing options...
David Saikin Posted May 2, 2014 Share Posted May 2, 2014 We are implementing gel blood type testing at one of our hospitals and I am running into all sorts of opinions of how to make up 6% albumin to use as a negative control for the reverse typing test. We don't think they will be able to come by AB plasma consistently so we are looking at other options. What are others doing? Do you use 22% bovine albumin and dilute with saline, or MTS diluent 2 or MTS Diluent 2 Plus? Or do you use 25% human albumin and dilute with saline or...? I use the cell diluent as my negative control for both the back type and IgG cards; if positive then either my cells or my diluent are contaminated (unless the card itself is funky). If you want to use albumin, the easiest way is to dilute 22% albumin 2:1 with blood bank saline. This comes out to a tad more than 7% but I think that is reasonable AND EASY to do. mla 1 Link to comment Share on other sites More sharing options...
galvania Posted May 2, 2014 Share Posted May 2, 2014 What cells are you using for your reverse? You could just juse a group A as a neg control for the A cells and B for the B cells....... Link to comment Share on other sites More sharing options...
Mabel Adams Posted May 2, 2014 Share Posted May 2, 2014 We will use Ortho's pre-diluted 0.8% reverse cells--Affirmagen. These small hospitals may not have a B patient in any given week. Using plasma seems to me like a better negative control but if using it makes the process of running QC really complicated and 6% albumin (or even the empty diluent --is that diluent 2 or 2 Plus, David?) will work adequately then I would consider it. I would like to have an adequate, cheap, simple negative control. Aren't both diluents LISS? Does that alter the reverse type to have it in both the reverse cells and the diluted albumin used as a negative plasma sample? Link to comment Share on other sites More sharing options...
David Saikin Posted May 2, 2014 Share Posted May 2, 2014 I use Gel Cell Diluent from Hemobioscience - it is the equivalent of MTS2+. I would use saline to dilute the albumin, not the cell diluent. Link to comment Share on other sites More sharing options...
Dansket Posted May 2, 2014 Share Posted May 2, 2014 (edited) Forgive me for being so dense, but why do you need a negative plasma control when doing ABO reverse grouping in Gel? Are you trying to demonstrate that the AFFIRMAGEN cells do not spontaneously agglutinate in the presence patient plasma? or Do you want do demonstrate that the AFFIRMAGEN cells do not spontaneously agglutinate in the Buffered Gel? I assume you are using and A/B/D Monoclonal and Reverse Grouping card. If appropriate patient plasma is not available, I would just add the AFFIRMAGEN cells to the Buffered Gel columns, centrifuge and read. There is your negative control. Edited May 2, 2014 by Dansket Link to comment Share on other sites More sharing options...
Mabel Adams Posted May 2, 2014 Share Posted May 2, 2014 From what I can gather, Ortho recommends using AB plasma or 6% albumin as a negative control. Otherwise, I agree with you, Dansket. 6% albumin mimics the protein content of plasma but that is about it. I guess using it is looking for whether the Affirmagen cells will spontaneously agglutinate in a mileau that has the same protein content as plasma? Maybe it just makes everyone feel better. Link to comment Share on other sites More sharing options...
Dansket Posted May 2, 2014 Share Posted May 2, 2014 Mabel, When do you run this control, Daily, Day of Use or with each patient? Dan Link to comment Share on other sites More sharing options...
David Saikin Posted May 5, 2014 Share Posted May 5, 2014 Mabel, When do you run this control, Daily, Day of Use or with each patient? DanIn the USA you have to run a positive and negative control with gel cards,i.e., you have to show that the cards react and don't react. Link to comment Share on other sites More sharing options...
Mabel Adams Posted May 6, 2014 Share Posted May 6, 2014 It is to be run each day of use. Link to comment Share on other sites More sharing options...
Abdulhameed Al-Attas Posted May 7, 2014 Share Posted May 7, 2014 (edited) As Anna, mentioned above ''You need to use a control that is equivalent to the reagents you are controlling''.that is to say, from the same manufacturer of your Anti-D. The Rh control contains everything that is present in the anti-D typing sera except the anti-D.If Rh control is not available then you can use from 6-8% Albumin Because the Albumin added to the Rh control is about 6-8%. And as David said above,The control is necessary (especially in apparently group AB positive patients).Another application is to use as a negative control when testing a Du or Weak D. Edited May 7, 2014 by Abdulhameed Al-Attas Malcolm Needs 1 Link to comment Share on other sites More sharing options...
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