yan xia 196 Posted February 20 Share Posted February 20 There are a lot of brilliant explanations, I learned so much from here. My guess about the pos DAT is that if the DAT(IgM) is positive, then it may cause false positive result in the D testing. This is why when we test a sample which is AB Dpos, we will run a neg control for the forward typing. Link to post Share on other sites
exlimey 383 Posted February 22 Share Posted February 22 On 2/19/2021 at 4:09 PM, diplomatic_scarf said: I disagree. Most gel cards and Anti-D reagents won't detect DVI for patients. Fortunately I can find numerous suitable quotes, because it's true. https://labs-inc.org/pdf/361_3.pdf Apologies. I was discussing reagents used in tubes, not gel cards. Link to post Share on other sites
Bijoux71 2 Posted March 8 Share Posted March 8 In scenarios like this, questions and possibilities that first come to mind are the following, not in any specific order: 1.) The DAT of the donor unit is extremely relevant. Yes, DATs are not routinely run on all donors however, when IRL testing discovers a discrepant IAT antigen type, Rh or other, a positive DAT is often the culprit. If the discrepant type is at immed spin or direct agglutination, several possibilities come to mind, such as a bacterial contaminated donor unit which may cause spontaneous agglutination, or a medicine that donor takes and is not screened for or donor doesn't give an accurate reply to pre-donation questions, or a donor with a cold agglutinin. 2.) Some previous posts have stated that most current Anti-D reagents will not detect DVI. I feel like that statement is confusing and should be worded to say "most Anti-D reagents will not detect type DVI by direct agglutination, but are able to detect DVI at IAT. yan xia and carolyn swickard 2 Link to post Share on other sites
Malcolm Needs ★ 4,910 Posted March 8 Share Posted March 8 21 minutes ago, Bijoux71 said: 2.) Some previous posts have stated that most current Anti-D reagents will not detect DVI. I feel like that statement is confusing and should be worded to say "most Anti-D reagents will not detect type DVI by direct agglutination, but are able to detect DVI at IAT. I think you would also need to re-word your comment slightly, as your comment, as written, is not valid in all countries. Within the BSH Guidelines for pre-transfusion compatibility procedures in blood transfusion laboratories (Milkins C, Berryman J, Cantwell C, Elliott C, Haggas R, Jones J, Rowley M, WIlliams M, Win N. Transfusion Medicine 2013; 23: 3-35) states, in paragraph 4.3.2 D typing, "i Where secure automation is used, D typing may be undertaken using a single IgM monoclonal anti-D reagent, which should not detect DVI. In the absence of secure automation, each sample should be tested in duplicate, either with the same reagent or with two different IgM monoclonal anti-D reagents; this is to reduce the risk of cross-contamination and the potential for procedural error where manual testing is undertaken." (my bold font). It will be seen that the Guidelines state "which should not detect DVI", rather than "which should not detect DVI by direct agglutination", and, having served for some time on the Transfusion Task Force of the British Society for Haematology, I can assure you (and others) that each and every phrase and sentence in any of these Guidelines is discussed to the nth degree to ensure that what is printed is what is meant. The Guideline goes on to give much more detail in terms of what testing or reagents should be used in different circumstances. Link to post Share on other sites
RichU 7 Posted Wednesday at 02:15 PM Share Posted Wednesday at 02:15 PM (edited) We use BioRad gelcards. There are different ABD-Confirmation cards for Donors and Patients. The Donor one detects DVI, the patient one does not. The D status of all donors found negative by the ABD conf card are confirmed using a monoclonal anti-D by IAT. Is re-grouping of units, sent from the blood bank, at the hospital a thing? Sorry for the late post Edited Wednesday at 02:24 PM by RichU Forgot to add question. Link to post Share on other sites
Malcolm Needs ★ 4,910 Posted Wednesday at 02:44 PM Share Posted Wednesday at 02:44 PM 28 minutes ago, RichU said: We use BioRad gelcards. There are different ABD-Confirmation cards for Donors and Patients. The Donor one detects DVI, the patient one does not. The D status of all donors found negative by the ABD conf card are confirmed using a monoclonal anti-D by IAT. Is re-grouping of units, sent from the blood bank, at the hospital a thing? Sorry for the late post Not in the UK. diplomatic_scarf 1 Link to post Share on other sites
AMcCord 1,289 Posted Thursday at 12:28 PM Share Posted Thursday at 12:28 PM 22 hours ago, RichU said: We use BioRad gelcards. There are different ABD-Confirmation cards for Donors and Patients. The Donor one detects DVI, the patient one does not. The D status of all donors found negative by the ABD conf card are confirmed using a monoclonal anti-D by IAT. Is re-grouping of units, sent from the blood bank, at the hospital a thing? Sorry for the late post In the U.S. we are required to confirm donor types at the hospital. Link to post Share on other sites
Joanne P. Scannell 228 Posted Thursday at 07:28 PM Share Posted Thursday at 07:28 PM I'm chuckling reading all of this because it's like the question, 'If the parents are both Group O, can they produce a Group A baby?' Ask a student, they'll say 'No way!'. Ask a BB fanatic, they'll say, 'Sure it could happen ... and here's how ...' And in this forum, there is never a simple answer! Malcolm Needs, diplomatic_scarf and AMcCord 1 2 Link to post Share on other sites
diplomatic_scarf 13 Posted Thursday at 08:06 PM Author Share Posted Thursday at 08:06 PM 36 minutes ago, Joanne P. Scannell said: I'm chuckling reading all of this because it's like the question, 'If the parents are both Group O, can they produce a Group A baby?' Ask a student, they'll say 'No way!'. Ask a BB fanatic, they'll say, 'Sure it could happen ... and here's how ...' And in this forum, there is never a simple answer! "Sure it could happen ,,, and here's how... one of the parents perhaps has the Bombay phenotype" Malcolm Needs and galvania 2 Link to post Share on other sites
galvania 804 Posted yesterday at 03:35 PM Share Posted yesterday at 03:35 PM or mum is a surrogate or baby is the result of an ivf with external donors Malcolm Needs, yan xia and carolyn swickard 3 Link to post Share on other sites
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now