noelrbrown

I just answered this question. My Score PASS  

This is an interesting topic and something i worked on many years ago. We would always do a DAT on suspected AIHA patients and would do the subclass DAT too to give us more information. The data we obtained was combined with CR51 assay data and the Hematologic picture. However usually and pretty quickly the DAT would max out at 4+ or 5+ depending on your facility scoring. This made it difficult to assess the efficacy of the drug regimen i.e. is it improving red cell survival or not. We used to quantitate IgG on the red cell using a radioligand assay to assess efficacy of drugs and guide the treatment. is this still done now? I imagine there is another assay instead of radioligand that quantitates IgG bound to the red cell?

Harvey would pretty much make anything, I hadn't realised he had passed away. One option is to ask a 3D printer person to make it but it would have to be a large printer or Dorinda Owens was or is based in Florida and makes custom racks, not sure if she is still in business i can never forget her email gelcellgirl@aol.com

Hemo bioscience sells a commercial IgG Anti D called MedTek it is supplied with a Certificate of analysis that gives the potency of the Anti D catalog number H801.

Thanks for letting us know, I worked with Imelda on a couple of projects when she was at Liverpool BTS. I always enjoyed her humour and approach to life. Noel

Hemo bioscience has developed a new polyagglutinability kit and is in the stages of validation and testing. Previously we had access to Tn polyagglutinable cells. However we lost access to the donor who had acquired Tn polyagglutinability. Does anyone have access to a patient or donor that is exhibiting Polyagglutinability? We manufacture Neuraminidase treated cells here so we have the general T polyagglutinability covered. Thanks in advance. Noel Brown President Hemobioscience Inc.

Sad news indeed, thanks for letting us know. Noel

If you subscribe to a whole Blood Proficiency test ( like CAP JAT) the samples contain reverse group antibodies anti A, Anti B etc. and a couple of antibodies. its easy to write your own study looking for carryover in the adjacent samples. Alternatively Hemo bioscience makes and sells a validation kit for instrumentation and this contains similar materials....

Thanks for letting us know, I worked with David a long time ago on an IgG quantitation assay.

Here is an example of how to calculate numbers of units to be screened from a Proficiency test we designed a couple of years ago. It makes a nice worked example. Frequencies and Donor Screening The frequency of the c (small) antigen is 80% in Caucasians, 96% in Blacks, and 47% in Asians (Refer to Table 3 for additional information regarding Rh antigen frequencies in various populations).3 Antigen Symbol Caucasian Black Asian D 85% 92% 99% C 68% 27% 93% E 29% 22% 39% c 80% 96% 47% e 98% 98% 96% Table 3: Common Rh blood group antigen frequencies3 The frequency of the K antigen in 9% in Caucasians and 2% in Blacks. The K antigen can be found at higher frequencies in specific populations—found in approximately 12% of Iranian Jews and can be found in up to 25% of the Arab population (Refer to Table 4 for additional information regarding the antigen frequencies associated with the Kell blood group system). Antigen Symbol Caucasian Black Other K 9% 2% Iranian Jews = 12% Arabs = As high as 25% k (small) 99.8% 100% Kpa 2% Less than 0.01% Kpb 100% 100% Jsa 0.01% 20% Jsb 100% 99% Table 4: Common Kell blood group antigen frequencies3 Problem #1: How many units of pRBCs should be crossmatched in order to find 3 antigen negative units? 1. Given that ~80% of the population will have the c (small) antigen, ~20% of the donors should be negative for the c (small) antigen. Additionally, given that ~9% of the population will have the K antigen, ~91% of donors should be negative for the K antigen. **NOTE: When multiple antigen frequencies are involved, you must multiply the antigen negative frequencies together. In this case we would multiple 20% and 91% to account for the donors who are c (small) negative and K negative. 2. Number of units to be crossmatched = x x = 3 units/0.18 = 16.7 (or 17 units) 3. The blood bank should crossmatch 17 units, where 3 of the 17 units should be compatible.

There is no licensed anti COb in the USA, i have a few vials of raw plasma in a freezer that i use for research purposes...

I suggest you try any high potency IgM anti D which these days is a monoclonal and thus not high protein. The anti D on your bench right now ( immediate spin) should work.

Add some Dextran to a plasma sample, it should do the trick and will save you a lot of time and effort freezing and retaining samples etc.

Hemo bioscience is a small biotechnology company based close to RTP has an opening for a Manufacturing Technologist to work in our laboratory and assist with manufacturing activities. The Manufacturing Technologist personnel participate in all non-analytical activities related to the manufacturing of Hemo bioscience products. This position is responsible for following standard operating procedures, best laboratory practices, current good manufacturing practices (cGMP) and current good documentation practices (cGDP) to ensure products are manufactured and systems are maintained to meet internal and customer specifications. Skills required: · Follows SOPs, best laboratory practices, current good manufacturing practices and current good documentation practices to ensure products are manufactured to meet specifications. · Participates in all non-analytical aspects of the manufacturing process from receipt of raw material to release of finished product. Activities include, but are not limited to: o Product label generation and printing o Product labeling and packaging o Product filling and capping o Lab inventory and cleaning o Temperature monitoring of refrigerated systems o Equipment maintenance o Specimen processing, non-analytical o Solutions preparation o Product manufacture · Responsible for documentation of all manufacturing related activities. · Provides suggestions for better production and staff performance. · Assists management for various production related duties. To perform this job successfully, an individual must be able to perform essential duties satisfactorily. The requirements listed below are representative of the knowledge, skill, and/or ability required. · Minimum Associates degree in a life science field or 1 years experience in an industrial cGMP or laboratory environment. · Bachelor’s degree in a life science field preferred Blood bank or Clinical Laboratory experience desirable but not required Ability to closely follow procedures and write reports clearly and concisely with good organizational skills. Close attention to detail. Knowledge of MS office programs. Please apply by submitting a copy of your resume to info@hemobioscience.com Salary is DOE, Benefits are provided

So.. just a quick word of warning, its easy to grind up seeds and do an extraction. But what i have found is that the extraction may or may not work and its a bit fickle. if you go down the path of making your own lectin you must QC it by using either polyagglutinable cells or at the very least prepare Neuraminidase treated cells. If you don't you could end up with false negatives.