noelrbrown

Sooooo, the action of eluting the antibody off the red cell can change the antibody structure, i recently saw this with an Anti Fya. I dont know the exact mechanism but imagine it has to do with the very low pH causing a change in the epitope.

It was Polyclonal material, I didnt detect the anti C or (G) on incoming inspection but did elute it off R2R2 Cells along with an Anti D. These days i stick to monoclonals.

Yes Anti G is very likely, BUT I once manufactured Anti D coated cells using R2R2 cells and was able to elute off a weak anti C along with an anti D, it was an odd antibody as it only reacted in Gel... i still believe it was an example of M-O.

Did you detect another (different) antibody in the eluate? eg. like an anti D ? If so you may want to consider Matuhasi-Ogata phenomenon....

Heterozygous versus Homozygous expression for the antigen is key especially in the MNS system. You should know what the status is of the cell you are using so that you can compare apples to apples.

Be aware that most Anti AB these days is a blend of anti A and Anti B monoclonal and is not strictly an anti AB.

Dear BBSLM, I am happy to walk you through some hints and tips in using the Hemo bio kit with gel cards, we use it all the time with Grifols and Ortho Gel cards here in our testing lab. The Hemo kit is very similar to the Immucor /Gamma kit as it is an acid elution method. However the Hemo kit is the only one that has been validated for use with gel cards by the manufacturer. our phone is 919 313 2888 and email is info@hemobioscience.com

You have to spin the final eluate very hard and for some time, try 5000 RPM for 5 minutes in a benchtop. We have validated our kit in Gel card systems. Other manufacturers kits may or may not be validated in a gel card. I suggest you read the IFU carefully and or call the manufacturer technical support.

If the plasma has Infectious disease state documents then HB may be interested in purchasing plasmas as we currently buy from commercial sources.

Which gel are you using and what is the source difference for the anti IgG i.e. monoclonal versus polyclonal or is it anti complement issue you are experiencing?

Have you tried EYlabs? They have a bunch of lectins (concentrate)

Can you explain further the potency statement "doing a potency this potency vary from 1:4 to 1:128." Do you mean with a AHG containing anti Complement?

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Hemo bioscience (USA) also sells the DTT ready to use (frozen) but I understand it is a long way from Portugal

Sounds like poor advice from the IRL, I think what they meant to say was that the CD38 antigen is weakly expressed on Cord cells. The Kell system is completely removed by DTT treatment so you should give K neg blood as you have no way to exclude anti K, BTW that also means JSa, Jsb, Cellano etc. also Lu null cells do not express CD38 either.