noelrbrown

You have to spin the final eluate very hard and for some time, try 5000 RPM for 5 minutes in a benchtop. We have validated our kit in Gel card systems. Other manufacturers kits may or may not be validated in a gel card. I suggest you read the IFU carefully and or call the manufacturer technical support.

If the plasma has Infectious disease state documents then HB may be interested in purchasing plasmas as we currently buy from commercial sources.

Which gel are you using and what is the source difference for the anti IgG i.e. monoclonal versus polyclonal or is it anti complement issue you are experiencing?

Have you tried EYlabs? They have a bunch of lectins (concentrate)

Can you explain further the potency statement "doing a potency this potency vary from 1:4 to 1:128." Do you mean with a AHG containing anti Complement?

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Hemo bioscience (USA) also sells the DTT ready to use (frozen) but I understand it is a long way from Portugal

Sounds like poor advice from the IRL, I think what they meant to say was that the CD38 antigen is weakly expressed on Cord cells. The Kell system is completely removed by DTT treatment so you should give K neg blood as you have no way to exclude anti K, BTW that also means JSa, Jsb, Cellano etc. also Lu null cells do not express CD38 either.

yes it is, Hemo bio sells it frozen ready to use and you can buy it powdered from other vendors. Noel

Unfortunately the answer is, it depends... If the anti CD47 is a human antibody, then it will cause a positive DAT as CD47 is expressed on most cells in the body including erythrocytes. If the anti CD47 is mouse or goat then it will not as this antibody will bind to CD47 but will not cause the same interference we see with Daratumamab. I believe recent therapies involve blocking some of the CD47 receptors.

Hemo bioscience is selling ready to use DTT ( frozen), see our web site for details.

Be careful as the diluents used to suspend the cells will be different. For gel card assays you need a Low Ionic strength diluent. Diluents often contain potentiators and will have been validated for use in the appropriate card system. I am not saying they won't work and they probably will however you should perform validation to ensure you don't miss a significant antibody.

Hemo bioscience is going to be launching ready for use DTT in the next 2-3 weeks. We plan on pushing it through our email list and will update our web site with product codes.

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No greater than 20% HCT is my advice