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Showing results for tags 'rouleaux'.
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I had a patient that had an ABO discrepancy. Testing is as follows: anti-A: 4+, anti-B: 4+, A1 cell: 1+s, B cell: 4+, Screen cells I.S.: all negative, LISS 37: all negative, poly AHG: all negative. Group O donors I.S.: negative, Group A donors: 1+ to 2+. Saline replacement for A1 and group A donors: negative. Microscopically, the A1 and A donors do not really appear as rouleaux. So the question is why do we see apparent rouleaux only with the A cells and not the screening cells?
Hello, I currently use Gel as my primary method. A tech on the weekend got the following: 3 cell screen: 2+ for all Gel panel A (11 cells) 1+ and 2+ reactions with the exception of 2 cells and the Auto control that were neg. Gel panel B (11 cells) 1+ and 2+ reactions with the exception of 3 cells that were neg. No pattern of single, multiple, multiples with dosage. Wells appeared to have thin sheet on the top of the column. Tube method immediate spin revealed Rouleaux and AHG results were negative. Saline Rep was done at immediate spin and reactions were removed. Is this a good enough, to prove that Rouleaux is causing the reactions in Gel? What would you recommend to indicate reactions are Rouleaux and not something else? Thanks, -Serafin