mpmiola

I am researching a good option to confirm the ABO typing of patients who are in the first care. We would like a bedside test, realized before infusing the blood into the patient. Does anyone know of a practical, quick and safe method that can be performed by nursing professionals, with no immunohematology laboratory experience? If you have any other ideas for patient safety and can share, I'll be grateful.

We left ice bars in the coolers to keep cold, we replaced at the time of preparation for transportation. we also use ice / barrier / blood / barrier / ice. Before doing that we had problem, now works well!

When nonavailable ABO-identical platelets. What to transfuse? Example: Patients of group A 1st choice: AB (incompatibility major) 2nd choice: B (incompatibility major) or 1st choice: O (incompatibility minor)

Why the first and not the others?

Yes, but c.261delG characterizes deleterious O alleles and is not present in alleles A. If this mutation is homozygous, I would suspect a cross-reactivity.

Thank you Baby Banker. If cardiac surgery is for extracorporeal, should red cells be washed if they present more than 24 hours of irradiation time?

I also agree with the subgroup of A. However, I would do other tests before considering the case as resolved. 1. Are the reagents you used from different manufacturers from the same clone? Do they have the same title? Differences in clone or titer may lead to differences in reactivity. 2. Tn antigens may be cross-reactive with some anti-A antibodies. Treatment of RBCs with the enzyme may help to exclude this interferant. 3. I would or would refer you to the molecular investigation to identify the deletion mutation at position 261 of exon 6, which characterizes O alleles. 4. Flow cytometry can also assist in the evaluation of the mixed field. Is it happening because of poor reactivity or chimerism?

Hello, I need your help with transfusion of packed red blood cells in pediatric cardiac surgery. Our surgeon requests washed and irradiated red blood cells for all children up to three months old and for children over three months, only if it is the second procedure. Did we ask why? However, he could not answer. It is important to say that we provide red blood cells of 5 to 7 days of storage, only that is 3-5 days post-irradiation and identical ABO. I believe it has no significant benefits. Some studies say that washing minimizes the risk of inflammation, but it is not a consensus. Other risks seem to me to be more worrying than the benefits pointed out, such as: Risk of contamination - our service does not have automation for the washing of red blood cells; Loss of yield - in addition to the loss of cells that occurs during the procedure, mechanical trauma can accelerate the process of hemolysis of more fragile cells; Anyway, I would like to know your opinion on this subject and what is the practice adopted by your service? Thanks for any input,

We only issue units that are 3-5 days post irradiation. Thanks a lot

Thank you, I'll do it.

John C. Staley, he asks for all children up to three months old and for children over three, only if it is the second procedure. When we asked for a referral for this conduct, he did not provide it! I believe it has no significant benefits. Some studies say that washing minimizes the risk of inflammation, but it is not a consensus. Other risks seem to me to be more worrying than the benefits pointed out, such as: Risk of contamination - our service does not have automation for the washing of red blood cells; Loss of yield - in addition to the loss of cells that occurs during the procedure, mechanical trauma can accelerate the process of hemolysis of more fragile cells; Anyway, I would like to know your opinion on this subject and what is the practice adopted by your service?

Hello guys, I need your help with transfusion of packed red blood cells in pediatric cardiac surgery. Our surgeon requests washed and irradiated red blood cells. Did we ask why? However, he could not answer. Someone knows what's best, red blood cells washed or not washed? It is important to say that we provide red blood cells of 5 to 7 days of storage and identical ABO. Thanks for any input,

I agree with Ciff and the Auditor that the best way is to transport it with cold material. This is how we do with external institutions to our Hemotherapy service. However, our inpatients are prepared for transfusion prior to delivery of the bags. When the nurse arrives with the bag, it usually does not take more than 5 minutes to install. So I have doubt if it really is necessary. Thank you for your contribution. Despite the difficulties we have ... we want to provide the best to our patients. I apologize for my poor English.

Yes, expect room temperature! Not necessarily the 30 minutes. This is the point! If we follow the guidance of the auditor and carry out the transport Cold (1-10), we will have to wait a little. Why not consider the transport time in RT?

The same nurse who transports, performs the transfusion. We have records of exit and start of the transfusion.