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Posts posted by SbbPerson
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As most of you know, there is a severe blood shortage nationwide(USA). Please donate it you can.
Thank you, you are heroes!
- Marilyn Plett, Cliff, AMcCord and 1 other
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On 4/1/2019 at 11:11 AM, butlermom said:
Does anyone know a vendor that sells a heat block that will reach a temperature of 120C? Our pathology department is looking for one.
Thanks.
Sorry, I know this question is 3 years old, but I found this link from Fisher, it lists incubators that can go up to 150C if needed. Good luck
https://www.fishersci.com/us/en/browse/90088096/dry-block-incubators
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31 minutes ago, Malcolm Needs said:
It is all over the place, to be honest.
It is Caucasian, rather than caucasian, It is group O, D Positive, and group A, D Positive, rather than either group O Positive or group A Positive (see the early editions of Peter Issitt's book).It is Oh (with a subscript "h"), and not "Bombay". The FUT1 gene, or, rather, the lack of a functional gene through various different genetic mutations, leads to the "Oh" phenotype, but this should NOT be called the "Bombay phenotype". Although this phenotype was first described by Bhende YM, Deshpande CK, Bhatia HM, Sanger R, Race RR, Morgan WTJ, Watkins WM. A “new” blood-group character related to the ABO system. Lancet 1952; i: 903-904. DOI: 10.1016/S0140-6736(52)92356-8, Another example of the Oh phenotype can be seen in the rare recessive condition, Leukocyte Adhesion Deficiency Type II where, to all intents and purposes, the patient will have a normal H gene, and yet the red cells are of the Oh phenotype, and anti-H can be found in the plasma. the phenotype has been identified in many different parts of the world (and is not just confined to mutations in India or even Asia (Hidalgo A, Ma S, Peired AJ, Weiss LA, Cunningham-Rundles C, Frenette PS. Insights into leukocyte adhesion deficiency type 2 from a novel mutation in the GDP-fucose transporter gene. Blood 2003; 101: 1705-1712. DOI: 10.1182/blood-2002-09-2840).
The other thing is, of course, that "Bombay" no longer exists - it is now Mumbai!I APOLOGISE FOR BEING A COMPLETE PEDANT!
No need for apology. Your input is great appreciated Malcolm. Thank you!
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4 hours ago, Malcolm Needs said:
If necessary, I am prepared to justify vote. I hope you are willing to justify your (or the wife's) incorrect nomenclature!!!!!!!!!!!!!!!!!!!!!!!!!!
Lol is it genomic? Or genotype? What would you call it Malcolm?
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For instance, take a caucasian man and his wife. Both type as O Positive, but the wife gave birth to an A positive baby.
The wife claims it is because she has Bombay. By genotype she is a Group A but serologically she types as O positive.
She refuse to consent to DNA testing. If she is lying, can she be charged with a crime?
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On 4/12/2019 at 9:27 AM, Bb_in_the_rain said:
For those of who works in transfusion service laboratory and would like to learn more reference cases, I can post some mock-up cases here. If you would like me to do it, please hit the "heart" button on this post. If enough folks want to practice case studies on reference lab cases, I can post mock-up cases here weekly or so..
I know this post is like 3 years old, but just wanted to say it would be nice to see some practice case studies in transfusion medicine. Thank you.
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On 11/28/2017 at 11:48 PM, margann said:
One of my staff was washing cells to do to do an elution but the cells wouldn’t spin down. The haematology indices are all normal and we tried it in another centrifuge but it was still red cells 3/4 of the way up the tube. In the end she split it into e tubes but even so it didn’t spin down as much as expected. Any explanations?
I know this question is like 5 years old, but better late than never. Anyways, it sounds like either there is something wrong with the sample or the centrifuge. If it a sample problem, request a redraw. That is just not normal. Good luck.
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On 9/26/2022 at 4:05 AM, CARMEN DELGADO said:
Just to clarify
is it safe to give blood components to patients before identifying the antigens present without having to use filters
juliedel23
All blood components must be transfused using transfusion tubing that has an inline filter. This filter helps removes clots, platelet clumps, and debris, etc. There are other filters that filter out WBCs, but those are not usually used at bedside. They are used during the manufacturing of blood components. So basically, all standard blood component transfusions to patients requires a filter.
So it is safe to give blood components to patients before identifying the antigens? No. You would need to know at the least the ABO/Rh antigens of the blood component before you can give it to any patient.
Good luck.
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3 minutes ago, SbbPerson said:
I just answered this question.
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My ScorePASS
Boy, I really liked this one! There are people who still use "heterozygous" and "homozygous" when describing antigens in red cell panels. When I come across a person who does this, although I understand what they are trying to say, it irks me a bit. Haha
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I just answered this question.
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My ScorePASS
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I use to work for a blood bank that QC'd expired panel cells when it is in use. We used the expired panels as selected cells, for rule outs/ins.
- exlimey, John C. Staley and donellda
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On 9/22/2022 at 1:33 PM, MMendoza said:
Hello,
We have just aquired two ortho Vision's we have successfully set up, connected to the LIS, validated, and tested one at one of our hospitals. However, the second site is having trouble even connecting to the LIS. We have ensured the settings are the same. We can ping the instrument from the Instrument server and vice versa. But we cannot telnet from instrument to IP and port on our instrument server. Our ports are open. Does anyone have any ideas or has this happened to anyone else? The Ortho technical keeps saying it is our network, however, firewall, security, ports, everything on our side has been checked so I am at a loss. Any ideas or feedback is extremely appreciated!!
Thank you!
Did you do a function check on the 2nd instrument? Since you can ping it, it appears to be connected to the LIS. So I am thinking it is a instrument problem. Tell those Ortho people you're a paying customer and they need to make sure you are happy with their service. Sorry, I couldn't be more help. Good luck.
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What are the filters for?
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I have never heard of that. What is LowT? I assume WB means whole blood?
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I am sorry, I know this post is about 3 years old. But I came upon it, and it peeked my interests. I googled the Sysmex Pochi because I have never used one before. It seems like a pretty amazing small hematology analyzer to me! Did you ask your oncologist what CBC/Diff results they need? Like what parts of the differential do they absolutely need? If the Pochi can provide that required information, why not just stick with Pochi? And save some money from not having to use the Coulter. Good luck.
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On 9/2/2022 at 9:23 AM, David Saikin said:
Thanks
for that info. We have ordered one of these.
Wow, I hope it works out well for you!
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12 hours ago, yayani said:
thanx for replay
yes we draw from actual blood and we are using CMIA ARCHITECT for screening donors
but we hosted an assessor that told us to use "another source" to repeat the test and he suggests to use a new specimen from blood bag .
thats why i am asking if someone else doing this!
Another source? Do you mean from a different blood draw? You would have to call in the patient to have the blood drawn again. But usually we don’t call patients back unless if we need more specimen or that there was a possible error in specimen processing.
I am not sure why would you need another specimen from the same blood unit. You collected the specimen tubes from the initial donor draw? So the assessor wants you to draw another specimen directly from the bag after it is collected already?
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On 8/31/2022 at 5:50 AM, Neil Blumberg said:
We use either ABO identical or washed O red cells. Usually volume reduced so the hematocrit is around 70-80% either by centrifugation or washing with Plasmalyte. We have data that saline washing is likely associated with more hemolysis and metabolic acidosis. Leukoreduced and <21 days old.
We prefer not to use the very short storage red cells (<7 days) as there is evidence they are more dangerous from randomized trials, albeit in mostly adult patients.
We do not CMV test or test for hemoglobin S, except for exchange transfusions. No evidence that hemoglobin S trait is a problem for transfusion in any situation, but particularly for smaller volume transfusions.
We do irradiate for newborns since immunodeficiencies, while very rare, are often not diagnosed until later in infancy or early childhood.
I think most places use a solubility test to screen for the sickle cells disease and the sickle cell trait. Although usually asymptomatic, sickle cell trait carriers can produce sickle cells in conditions of low oxygen tension. And of course, sickle cells have poorer oxygen carrying capacity than normal cells.
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On 9/6/2022 at 9:59 AM, rmilford said:
I'm wondering if it is valuable to get my SBB. If I have an MLS and 2 years of experience in a very busy, vey well-run hospital blood bank, will completing an SBB program give me additional knowledge that will actually help me run my current blood bank? Can anyone comment on the quality of online programs available? I see programs that are available online and accredited by CAAHEP include: UTMB Health, BioBridge Global, LifeShare Blood Center, Rush University, and oneblood.
It will depend on your needs and preferences? Would you prefer distance learning(online) or classroom learning? I got my SBB through Rush University, and it was a very rewarding and enrichening experience! I really enjoyed it!
Here is a list of SBB programs and their admission requirements. Good luck!
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On 9/8/2022 at 5:01 AM, jayinsat said:
@rmilford, have you considered the DCLS program at UTMB? Since you are considering the SBB, which I think is worth the knowledge alone, you may find that the DCLS program will do the same and more, giving you a terminal degree. I anticipate the role of DCLS will become important in healthcare in the near future. It also opens up more possibilities outside traditional lab roles, including teaching higher education.
Just another thought.
UTMB also has a Masters of Science Transfusion Medicine(MSTM) program, if you are more interested in furthering your blood banking education. If you already completed an accredited SBB program, you would only need like 24 credits to get your MSTM. I finished 6 credits already, so I have only18 more to go.
Like everybody else has mentioned here, it will open more doors for you. Good luck
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Is the specimen from the actual blood donation draw? Are you using serologic methods? I assume yes for these 2 questions. All reactive initial results need to be repeated in duplicate. Interpretation is based on best of 2 out of 3. For example your initial result is reactive, 2nd is reactive, but 3rd is non-reactive. You're interpretation is reactive.
Then send out the donor specimen for confirmatory testing. Good luck.
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I think we give the freshest blood because it is at its most effective for oxygen carrying capacity, since pH level may drop due to possible cell lysis during storage. We basically do the same thing as mentioned above by several people, except we also do HbS testing on the RBC unit. We want to give the freshest and best oxygen-carrying red cells to our neonatal patients in need.
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I apologize this is a dumb question. Why should there be 3 days between type and screens if a patient requires blood? What can happen in 3 days? Thank you in advance, I appreciate your time and knowledge 🙌🏽👍🏽
Mock-up cases
in Case Studies
Posted · Edited by SbbPerson
spelling
What did it mean at the end, with the "blank, blank" ? And why would the doctor ever advised the patient to get rid of her antibody card? That was odd, considering that she did have a history of antibodies.
I have not seen many patients here in America with an Antibody card. The last one I encountered showed me her card when I drew her type and screen. This was very helpful for me, considering I was a one-man show! I did all the blood collecting and testing! I appreciate all the help I can get.