Jump to content
Eagle Eye

Antigen typing on recently transfused patient

Recommended Posts

How do you perform antigen typing on recently transfused patient?

Do you use monoclonal reagents for antigen typing for recently transfused patient? Explain the reason for using monoclonal reagents?


Share this post

Link to post
Share on other sites

Gel testing will display mixed cell, so we use that method when possible.  If mixed cell reactivity is seen, we cannot interpret the results (or evaluate with caution taking into account how much blood was transfused, sometimes there is a tiny amount of positive/negative cells = transfused cells).

Share this post

Link to post
Share on other sites

We let our reference lab deal with that problem. They have been typing using cell separation (which is kind of fun to do), but are moving toward molecular depending on the case. Molecular is still too pricey for using routinely.

Share this post

Link to post
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now

  • Similar Content

    • By dmed03
      Trying to get the antigen typings on blood bag units to auto fill into Meditech from the barcode. Any ideas on how to do this?
    • By mollyredone
      I know you are not supposed to antigen type anyone who had been transfused in the last three months, but what someone who is pregnant?  Seems like those two go together a lot, but if it is a simple immediate spin or RT incubation, would it be accurate if she is showing a new antibody right before delivery?  She already had an anti-E and today, for her C-section, and is showing an anti-c.  I was going to antigen type her but couldn't find any info about whether it would be valid or not.
    • By seraph44
      Hello Blood Bankers,
      I recently ran into my first unsatisfactory Antigen typing QC. A tech performed QC for an "e" antisera using a heterozygous cell. Results were negative at IS and negative after 5 min incubation. This was repeated by the same tech and later repeated by myself:Results did not change.
      The "e" antisera was ran with a different heterozygous cell and results were negative at IS and 2+ after 5 min incubation. Both the cells and the antisera were near expiration date. However, I'm a bit confused as to what corrective action is needed. How do I prove my panel cells are working correctly and how do I prove my antisera is working correctly?
      I think I proved my antisera is working correctly by running it with another Hetero cell. Should I run my panel cell with a new antisera to prove the panel is "e" positive?
      Thank you,
    • By BBNBHM
      Does anyone know of any standards that require repeat of special antigen typing that was performed by a reference lab.  At this time, the facility I work at will repeat any special antigen typing that was performed by the ARC and then documents this information in the computer system (and on paper) and places their own special antigen stickers on the units in addition to the tag attached by the ARC.  Any info would be greatly appreciated!  Thanks!
  • Advertisement

  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.