I know you are not supposed to antigen type anyone who had been transfused in the last three months, but what someone who is pregnant? Seems like those two go together a lot, but if it is a simple immediate spin or RT incubation, would it be accurate if she is showing a new antibody right before delivery? She already had an anti-E and today, for her C-section, and is showing an anti-c. I was going to antigen type her but couldn't find any info about whether it would be valid or not.
Hello Blood Bankers,
I recently ran into my first unsatisfactory Antigen typing QC. A tech performed QC for an "e" antisera using a heterozygous cell. Results were negative at IS and negative after 5 min incubation. This was repeated by the same tech and later repeated by myself:Results did not change.
The "e" antisera was ran with a different heterozygous cell and results were negative at IS and 2+ after 5 min incubation. Both the cells and the antisera were near expiration date. However, I'm a bit confused as to what corrective action is needed. How do I prove my panel cells are working correctly and how do I prove my antisera is working correctly?
I think I proved my antisera is working correctly by running it with another Hetero cell. Should I run my panel cell with a new antisera to prove the panel is "e" positive?
Does anyone know of any standards that require repeat of special antigen typing that was performed by a reference lab. At this time, the facility I work at will repeat any special antigen typing that was performed by the ARC and then documents this information in the computer system (and on paper) and places their own special antigen stickers on the units in addition to the tag attached by the ARC. Any info would be greatly appreciated! Thanks!