How do you perform antigen typing on recently transfused patient?

Do you use monoclonal reagents for antigen typing for recently transfused patient? Explain the reason for using monoclonal reagents?

 

We don't antigen type recently transfused patients.

We wouldn't antigen type recently transfused patients either.  We do not guess - we genotype, in the full knowledge that the genotype does not always match the phenotype.

Gel testing will display mixed cell, so we use that method when possible.  If mixed cell reactivity is seen, we cannot interpret the results (or evaluate with caution taking into account how much blood was transfused, sometimes there is a tiny amount of positive/negative cells = transfused cells).

We let our reference lab deal with that problem. They have been typing using cell separation (which is kind of fun to do), but are moving toward molecular depending on the case. Molecular is still too pricey for using routinely.