Grifol Antisera's and red cells antibody screening kit

[salman 1]

 Is any one having experience about "Grifol" blood bank Antiser's / Antibody identification pannels etc.?

Pls share your experience,

[Auntie-D 363]

I'm not sure what you are asking. Is there a problem at the moment with the reagents?

[salman 1]

Sorry for late reply,

we are planning to replace Biorad to grifol antisera's

[cthherbal 54]

I don't know anyone who is using them, yet.

[David Saikin 1,361]

I have just "played" with the Grifols manual gel system and reagent red cells.

Red Cells/antibody panel:  What to say about reagent red cells - they work as anticipated.  Their 11 cell panel has a very nice antigram including Kpa and Jsa positive cells (don't know if the Jsa+ would be on all lot#s.

Have not used any of their antisera, but have tested antigen typing in their gel using "classic" reagent abs.  Everything I tested (monoclonal C,c,E,e,K,Jka,Jkb, and M; and polyclonal k,Kpa,Fya, Fyb,S,s and Lectins: A1 and N) worked in their gel (as they do in Ortho's).  I use a variety of vendor antisera from Ortho, Immucor, Hemobioscience and Quotient.  I found Immucor's monoclonal anti-P reacted with P negative cells (as it does with the Ortho gel also).

For what it is worth . . .

[tricore 150]

Our company is switching to Grifols. They are starting to validate. Manual methods and all three instruments. I will keep you posted if there any problems, do not expect any.

[Belial 0]

On Friday, February 20, 2015 at 4:45 AM, salman said:

Sorry for late reply,

we are planning to replace Biorad to grifol antisera's

I can understand your decision. the quality of bioraed is going down. I use also Grifols and it is good stuff.

[tricore 150]

One of our hospitals just came up on the Grifols Instruments - Erytra, Wadiana, and DG Reader.

The techs I queried at the 2 largest hospitals like the Grifols reagents. They like the reactions - they are "crisper".

[David Saikin 1,361]

You definitely do not find the anti-M's like you do with the Ortho gel.  I had a few frozen and they did not react with the Grifols system.