Use of monospecific IgG or polyspecfic Reagent in IAT?

[Desoki]

I want satisfied answer about what is more better use of monospecific IgG or polyspecfic Reagent in IAT whatever tube method or gell card, and why?

very thanks for your cooperation

[Yanxia]

If the specimen is EDTA anticoagulation , I will use monospecific IgG reagent, because the complement activation is suppressed by EDTA chelation Mg2+ and Ca2+; and if the specimen is not EDTA's, I will use polyspecific reagent to detect the complement dependent antibodies.

[David Saikin]

I use anti-IgG unless I am performing a Direct Coombs' test, in which case I also use anti-C3b,-C3d. We do not stock polyspecific antiglobulin.

[Malcolm Needs ☆]

In most cases, we use polyspecific AHG (simply because it is cheaper), but Shily is quite correct in what she says.

If we have a stubborn auto-antibody, however, we will more often than not use a monospecific anti-IgG reagent (despite the cost) because we tend to get better, clearer results that way.

[David Saikin]

Malcolm - do you do your testing in gel cards (poly cost more than IgG?)?

In most cases, we use polyspecific AHG (simply because it is cheaper), but Shily is quite correct in what she says.

If we have a stubborn auto-antibody, however, we will more often than not use a monospecific anti-IgG reagent (despite the cost) because we tend to get better, clearer results that way.

[Malcolm Needs ☆]

You are absolutely correct about the cards David.

My mind was working in two directions at once, and I posted a load of nonsense really (nothing unusual there then)!!!!!!!!!!!!!!!!!!!!!!

No, I was thinking more in terms of the liquid AHG we use for the tube techniques.

[Losmeister]

Our specimens are always EDTA.

We use mono-specific IgG for IAT.

[Likewine99]

We are the same as Losmeister

[Brenda K Hutson]

I remember many years ago when we switched from Poly to IgG. Made life so much easier!

Brenda Hutson, CLS(ASCP)SBB

[EDibble]

We use the ECHO for most IAT testing but use tubes as our backup. We use IgG