Losmeister

Been donating since High School. Its one of the most generous anonymous goodwill deeds a human animal can do. Since HS, mostly rbc's, but about 25 aphersis platelet donations in the last 3 yrs. Work in the field drives home the necessity. And if you wait for the next guy ( or gal for that matter ) to do the right thing, you might wait a long time...

my response is not so much response as it is an addition to the originally posed question a) of the SBB's that currently visit this forum can anyone forward an opinion on 1) the best ONLINE program available and 2) the best work/study program?? I have been thinking of taking this trun in my career currently I am licensed in California as a Clinical Immunohematologist and working at http://www.bbr.org/ any and all info would be immensely appreciated!

Our specimens are always EDTA. We use mono-specific IgG for IAT.

After reading a thread re:WAAs and the use of PEG for autoadsorptions our lab here is considering adopting this method. It will however lead us to use much higher volumes of PEG which we are now purchasing by a manufacturer. Do any of you all make your own 20% PEG solution? If so, your recipe and recommended expiry date would be much appreciated. My presumption is that the QC would be similar to what the package insert for the commerically prepared solutions would be. With the exception that 4 drops be used in lieu of two. Any and all response will be greatly welcomed.

Indeed, the phenotypes you describe were the crux of the original post. Neither the current Harmening's Modern BB and Transfusion Parctices, nor the current AABB manual made any mention of the original ID in 1953 nor listed all the potential phenotypes that could make allo-anti-f. My understanding of the way the "compound" antigen functions in the end proven sound. The article also points out how there are cases where the f antigen is expressed WITHOUT the presence of e.

We have, on occasion, used some of the ALBA anti-sera and found it quite good.

I was informed today by my technical director that they, Immucor, were increasing reagent prices... somewhere north of 50%

"According the author cDe/cDE individuals can produce f antigen not anti-f except autoantibody."

"According the author cDe/cDE individuals can produce f antigen not anti-f except autoantibody." I think cDe/cDE EXPRESS the f antigen, therefore can only produce an auto ANTIBODY.

The article answered my question. Forum adminstrator can feel free to delete thread.

An adendum... Perhaps the way I phrased the latter portion of the above may make one reluctant to respond. Its most expressly NOT MY WISH to create an adversarial relation with my colleagues, or put anyone on this forum in an in-between position. A recent search yielded the following article... Anti-f in a 24 yr old male... http://findarticles.com/p/articles/mi_qa3890/is_201004/ai_n53930552/?tag=content;col1 in this case the patient expresses c, but not e. " Then the antibody identification process was completed by antigen typing the patient's red blood cells for c and e antigens. The cells were c-positive and e-nagtive as shown on Table 3." (for a reason unknown to me none of the tables cited in this online version are viewable) Can the conclusion be drawn that it is therefore possible for a patient who lacks the c antigen but expresses the e antigen to form an anti-f antibody?

recently our lab rec'd a specimen for Ab ID on a patient who was known to have an anti-K. This time, however, she also had a strong COLD AGGLUTININ and 2+ coombs ( Igg ++, POLY ++ C3-) So... a REST adsorption was perfomed and it looked to me that she had developed an anti-f to go along w/ her previous anti-K. ( clearcut rxns in 10/16 panel cells,a homozygous c+ cell was NOT reactive) Now, the patient is O pos, C+, E-, c-, e+ , and this gave me pause, but my understanding of the Anti-f antibody is as follows- the epitope is created of the juxtaposition ( cis position) of the c and e antigens, thus Dce/DCE WILL react, while DcE/DCe will NOT. So, my question is whether or not the literature, and collective experience dictates that a patient MUST be negative for BOTH c and e for a patient to develop an anti-f. My understanding tells me my call is reasonable while the senior tech and our technical advisor wish for the report to be changed. ( Call it an anti-c) Either way our recommendation was for IAT crossmatch compatible blood lacking the K and c antigens.

I had the pleasure of seeing Dr. Garratty speak a couple years back... however, I am googling ( no, not drooling) like mad and not finding anything about this teleconference series...

question- RE: Elution to attempt to see whether an auto-Ab has specificity Is it acceptable to do a PEG autoadsorption, THEN Elute?

[ATTACH=CONFIG]388[/ATTACH]Get Smart!

"my insistence on understanding " thats just good science If ya ask me... um, which you didn't...

Pos DAT flashed through my mind before a weak D expression of some sort... just percentages in my own experience, I guess...

Liz- I believe David refers to AABB method 4-10 Polyethylene Glycol Adsorption

As for the comment above - the flippant/jokey tone was easily indentified for me... same way I used to feel about WARM AUTOS... just send and wait... jajaja! Anyhoots, am awaiting fruther word from Lisa as to how their patient tolerated the washed units....

The temp spikes reported by Lalamb is the first I'd heard of this. More detailed info and patient hx is something I've missed being once removed into the reference laboratory as opposed to the xfusion services in the hospitals I was employed at previously. We've crossmatched ~40 platelets for this patient w/ 100% compatability, so he's doesn't SEEM to have platelet antibodies. As for going K- preemptively, I'll bring it up to our technical director, its a logical extension to the way we treat the 5 major Rh antigens presently.

Hello... actually I am a sometime lurker who works at the Ref Lab that Lalamb sends specimens to... ( 20 yrs BB experience ( as well as Hemo, Chem et cet) , 3rd yr at Ref Lab at a local Blood Bank- see http://www.bbr.org/ ) Patient has developed an anti-K ( see above # of units since April 2010, no wonder...) Malcolm stated above and I concur, washing is a good idea if only to see whether or not it helps at all...

I thought CRAP was cold reactive antibody phenomena..