SandyR Posted February 2, 2007 Share Posted February 2, 2007 Could anyone share what they use as an Rh control when doing tube testing? We only incorporate an Rh control when we have an AB person.Thank you Link to comment Share on other sites More sharing options...
Mabel Adams Posted February 3, 2007 Share Posted February 3, 2007 We use Gammaclone control. (Our typing reagents are all Gammaclone.) You can use 6% albumin and I think some use saline. Link to comment Share on other sites More sharing options...
Eagle Eye Posted February 3, 2007 Share Posted February 3, 2007 we use 6% albumin for Rh control. Link to comment Share on other sites More sharing options...
SandyR Posted February 5, 2007 Author Share Posted February 5, 2007 Do you dilute your own 6% albumin or can you buy this product?Thanks Link to comment Share on other sites More sharing options...
donellda Posted February 6, 2007 Share Posted February 6, 2007 We generally use a 6% albumin solution that we prepare from 22% albumin. Patient's serum can also be used or any commercial antisera that gives a negative reaction. For AB Pos patients we generally use the 6%albumin or serum. Link to comment Share on other sites More sharing options...
Eagle Eye Posted February 7, 2007 Share Posted February 7, 2007 Do you dilute your own 6% albumin or can you buy this product? Thanks We prepare 6% from 22% albumin. Link to comment Share on other sites More sharing options...
JAlvarez Posted February 13, 2007 Share Posted February 13, 2007 We also use 6% albumin diluted from the 22%. Link to comment Share on other sites More sharing options...
vdvictory Posted April 22, 2009 Share Posted April 22, 2009 We also use 6% albumin diluted from the 22%. When you dilute it yourself what do you use for the expiration date? Does it have to be made every day or can it be used until it is all gone? What do you use as the lot number? We currently buy Ortho Rh control that is more than $1000 a box so we want to change to making our own. Thanks for your help!!! Link to comment Share on other sites More sharing options...
sdweaver29 Posted April 23, 2009 Share Posted April 23, 2009 Saline is our choice for Rh control, and economical. Link to comment Share on other sites More sharing options...
galvania Posted April 23, 2009 Share Posted April 23, 2009 You really should use a proprietary Rh control. The control should be EXACTLY the same formulation as the anti-D (minus the antibody, of course!) - not only with respect to albumin concentration, but other components of the buffer as well. Otherwise you could be missing false positives due to spontaneous agglutination/aggregation with one of the buffer componentsAnna Link to comment Share on other sites More sharing options...
Eoin Posted April 23, 2009 Share Posted April 23, 2009 (edited) Agree wholeheartedly with Galvania.Inspectors over here would insist on the same formulation for Rh control (only minus Ab). This thread goes back to 2005 - interesting. Are we under pressure to do it "on the cheap"?RegardsEoin Edited April 23, 2009 by Eoin Link to comment Share on other sites More sharing options...
eeagan Posted April 23, 2009 Share Posted April 23, 2009 For those of you who use the Ortho Rh control, be aware that this control is only for the Ortho human based anti-D. It is not for the Ortho monoclonal anti-D. If you are using the monoclonal anti-D, you should use 6% albumin, saline or the patien'ts serum/plasma for the control.We use a Rh control (6% albumin) only for AB positive patients when they are typed manually. We use the Ortho ProVue. So all patients typed on that have a Rh control as part fo the typing. Link to comment Share on other sites More sharing options...
SandyR Posted April 23, 2009 Author Share Posted April 23, 2009 I would love to use a proprietary Rh control but I don't see that Ortho offers one for their BioClone Anti-D tube test. If I'm missing something please let me know Link to comment Share on other sites More sharing options...
eeagan Posted April 23, 2009 Share Posted April 23, 2009 Sandy, You are not missing anything. Ortho does not have a Rh control for their Bioclone anti-D. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted April 23, 2009 Share Posted April 23, 2009 Galvania and Eoin are quite correct in my opinion.If you do not use such a negative control, but use things like saline, albumin, saline and albumin, etc, you are not using a negative control; you are using a sop (and I don't mean a Standard Operating Procedure!) to make you think you are using a negative control. It gives you peace of mind when, in reality, you should be extremely nervous! Link to comment Share on other sites More sharing options...
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