sdweaver29

Can you send me a copy? Sdweaver29@msn.com

Please tell me why staff prefer to use the Provue over Echo. I am working on the Echo validation.

Can you exPlain to me what kind of samPles would be foamy? Overall, what kind of performance do you get by having automation? If techs are not thinking through the process, with automation they will totally forget how to think as most get. Omfortable. We have had a provue for a few years now, now techs fear doing anything the tube method

I am glad to hear that others are seen the same problem I have been fighting for quite sometime. As a matter of fact the whole thing got to be too much that I loss confidence on the instrumen. Which is soon to be replaced, mostly because Ortho forgot what matter most- THE CUSTOMER.

thanks Malcom, I am honored by seen you respond to my post. I have visited this site regularly fo r a couple of years and have learned soo much just by reading everyone's post and reponses. I did decide to place a message on patient;s chart to transfuse compatible Rh negative , C negative packed cells. I am just very puzzle by the fact that this patient is actually weak Du, has developed a anti-D and that has also sensitized his cells. The eluate show the anti-D and C pattern but also the rest of the cells were very weakly positve caused by the warm. I would like to also share another case that I encountered today. Patient was previously identified with anti-K andanti-E. Since patient has received K,E negative units. Last transfusion was 11/2011. Patient returned yesterday for a another transfusion. The panels showed the previously identified antibodies with a positive autp control. DAT's were performed and reported as positive. The eluate, according to tech performing testing indicates anti-K recovered. Is this possible? I am very incomfortable with all this as the staff that work the off hours are generalists.

I agree with magnum, that would make too much sense.

Can someone offer some ideas on this case: 49 yo male. going for back surgery. Sample received for type and screen. patient is O Du positive with positive control. Antibody screen all three cells negative. Panel positve all the way, positive auto control. DAT positive, Igg positive, C3 negative. Eluate shows a WARM with a anti-D. Warm adsortion performe and anti-D and anti-C recovered. Patient antigen typed for C negative. Patient was originally typed as D positive (2+) and transfused 4 units O positive back in 2004. I thought that anti G was produced by individuals that were D negative. If it C and D how is it possible to have a positive DAT and recover anti-D from the aluate? There is no Winrho or diagosis of Cancer.

I have a similar issue. Gel antibody screen negative, therefore an immediate spin crossmatch done but all units attempted were incomaptible 2+. Carried to 37 and reactions are still present, although weaker. Now negative at coombs. Short cold shows positive for cold agglutinin. If my REST adsorption is negative screen at 4C, where does that leave me with the crossmatch. The original crossmatch shows incompatible at 37 which I consider significant. Is it acceptable to report as incompatible due to cold rather than prewarm crossmatch and then report compatible. I really have a hard time just reporting compatible after prewarm technique. The evening staff is all generalist and they are too quick to jump on prewarm technique without investigating the cause of the reactions. Does any one have a good flow chart for working with cold and the best approach to handle the crossmatch and reporting? I would greatly appreciate any help here from those blood bankers that have more experience.

I had the no reaction problems with exactly the same lot number while doing my proficiency. I called Immucor and they did confirmed they had had some reports of very weak or negative reactions. They overnight me a new lot of complement check cells. They work but still very weak reaction. I really had to incubate them for 10 minutes at room temperature after adding the check cells before centrifugation.

I wanted to go soo bad but our staffing situation at our facility got bad I had to cancel.:cries::cries:

Barb, I would greatly appreciate if you could share your copy of the SBB exam review. My email is sdweaver29@msn.com

I would appreciate a copy of the 2007 SBB exam review. my email address is sdweaver29@msn.com

Yes, we do send the FFP to OR n cooler to include a temperature monitor. If plasma is not transfused we can return it to our inventory and utilize for another patient within 24 hours of thaw

We apply the same criteria to thaw plasma as we do to pack cells. We always take temperatures when units come back even if under the 30 minutes. I have found that it may not take long for the unit to fall outside the 10C shipping temperatures if they are not issued in a validated cooler. I have seen more this summer and part may be due to the extreme temperatures we are facing here.:cool:

We have just gone thru the false positive #2 cell and negative panel. Now with the new lots I see a different problems. Patient was identified with ANti-kell just last week. Then this weekend while working a new sample for crossmatch I went ahead and set the patient on provue to run antibody screen along with panel to save time. To my surprise, the antibody screen was negative but my panel was positive. The panel showed Kell in addition to something else. This sample was ran with the exact same lot of screening cells and panels. Has anyone seen anything like this?

I have had the exact same problem with my previous lot wich expired 07/24/09. I contacted Ortho but they were not able to provide a clear answer. They did tell me that they hace changed formulation of the 0.8%, they removed the antibiotic and for some reason now the cells are more sensitive to light. Noothing they have told me so far explains the problem. It is erratic because you see the problem with one patient but not others. I am really loosing confidence with Provue.

We use Soft for our LIS, we have our provue interface to when you query the orders if the untis have not been retyped there is one schedule. But, becaful we have had three incidents where the provue totaly misinterpreted results. I have submmitted the data to J&J but have not heard anything.

Saline is our choice for Rh control, and economical.

I am really pleased to see that other techs are seen the same problems in reference to WARM autoantibodies. I think we may be getting to comfortable calling things WARM and the gel technology seems to have created a lot of confusion. Our facility follows similar protocols as you have described.