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Found 11 results

  1. How long does RHIG really persist? The package insert says one thing, but with Rh-loving methods such as solid phase, I feel like I see RHIG hang around a lot longer. I think this has implication for pregnant mothers who have suffered from trauma or miscarriage prior to their current pregnancy. Thoughts?
  2. Hi All, I was wondering if antibody titre is performed on a pregnant mother who previously had HDFN. According to the books, it mentions 'After the first affected pregnancy, the antibody titer is no longer useful'. Therefore does it mean that it doesn't matter what the antibody titre level is, and should be referred to fetal medicine specialist regardless? Or if there is more to this, I would be grateful for some enlightenment
  3. We currently have a 50 year old male in house that had an accident that damaged his foot 3 weeks ago. He arrived septic and has had to have an amputation. His ABO/Rh gives a B pos with a 4+ anti-D. His gel screen and panel give 1+ results that match up with an anti-D (all others rules out). His autocontrol was positive at 1+ by IgG, neg for compliment. The eluate results matched the original antibody ID. Presently this patient's specimen is on its way to our reference lab. Previous history at another facility lists him as B Pos, screen negative. As far as we know, he has never been
  4. Has anyone seen an Anti-D go from negative to 2+ positive to negative? We had negative antibody screens on an elderly A Negative woman from 2012 through 2018. 8 RBCs were transfused during this time. In August 2018, the antibody screen was 2+ positive, Anti-D was identified, and she received 1 RBC. In April 2019, the antibody screen was negative on 2 different occasions. The possibility of the August 2018 specimen being the wrong patient seems unlikely since we use hand-labeled separately armbanded specimens. However, I have never seen a true Anti-D behave this way.
  5. Mrs X Para 2+1 with dichorionic diamniotic twins. Anti-D+G present Anti-D quantitation levels at 13+2 and 29+3 weeks were 21IU/mL-1 and 330IU/mL-1 respectively. Mum O RhD Positive rr, Dad A Rh D Positive R2Ro Twin 1: A RhD Positive DAT 4+, did not go over 1.5MoMs on middle cerebral artery Doppler peak systolic velocity surveillance and did NOT require IUT. Postnatally only required top up Tx’s Twin 2: O RhD Positive R2r DAT 4+. required three intrauterine transfusions. Postnatally required Exchange Tx, IVIg and phototx and serial top-ups Although both twins in this case were RhD positive
  6. What are other people's institutions practices on the following. If you have a patient with an anti-D do you need to go ahead and carry out the D antigen typing on the patients rbcs through the IAT phase(weak D testing)? The AABB 18TH ed. Technical Manual states on pg. 327 "When the D type of a patient is determined, a weak D test is not necessary except to assess the red cells of an infant whose mother is at risk of D immunization." It then goes on to say under Identification of Antibodies to Red Cell Antigens pg.401 "Determining the phenotype of the autologous red cells is an important part
  7. Hi all, I'm trying to find out the similarities and differences around the world (but particularly in Europe) for guidelines related to measuring fetomaternal haemorrhages and issue of anti-D. I'm particularly interested in which countries issue anti-D and don't estimate the FMH, what formulas countries use for calculating doses, what tests different countries use, whether the FMH is measured in whole blood or packed cells, whether different countries use the 1.22 muiltiplier to account for the different sizes of red cells... Not a small list I know. I've found a few internation
  8. Hi All, So I would like to present a scenario that happened to me and get your input. I received a specimen from the ED for ABO/Rh testing on a young female (she had a miscarriage, which at the time I was not aware). We use the BD Pink (EDTA) blood bank tubes for all of our blood bank testing, this particular sample was about a little more than 1/4 of the way full (yes not the best sample, learned my lesson with this case) - there were no visible clots in the tube or in the cell suspension I made for testing (testing was done fairly quickly since it was only an ABO/Rh and we use a STATSpi
  9. We are dealing with a 24 day old baby who just had his second top up transfusion. He was born at term to an A neg mom who was discovered at that time to have anti-D. Her titer was 32. She had a negative antibody screen at the beginning of the pregnancy but was not tested when she got her 28 week dose of RhIG. The baby was A pos and had a strongly pos DAT. These results triggered us to test the mom and find the anti-D (we don't do routine screens on all OB admits). The baby had a Hct at birth of 40, so a bit low but not worrisome. He was under bili lights for 5 days and went home with a
  10. Dear All I am writing a paper and I am finding difficulty sourcing a reference for the following statement I am making in the paper. I know I have read it somewhere but for some reason I cannot find it now Background: once an Intrauterine transfusion has happened, the transfusion procedure itself is known to increase antibody levels. Therefore carrying out serial quantitations post IUT are unnecessary as the patient will be closely monitored by MCA Doppler US. Best wishes John
  11. We have a patient who received many units of group/type specific red cells in Jan. of this year. At that time, his antibody screen was negative. His group and type is A, Rh positive using monoclonal typing reagents. The anti-D reactions have been 2+ and 3+. His current specimen shows an anti-E in the plasma and a panagglutinin in the eluate tested in gel, with stronger reactions in the D+ cells. His eluate tested in tube shows a clear anti-D pattern. Could this be a D variant? Auto-anti-D? Should he get Rh negative red cells? Thanks!
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