Jump to content

Transfusion Reaction Work-ups


mrmic
 Share

Recommended Posts

I am curious to find out what methods other Microbiology Labs are using for investigating Transfusion Reactions for the Blood Bank Transfusion Service?  Limited verbal communications suggest there are a lot of variations out there.   Based on product, how do you screen for Salmonella, etc., Staph aureus, Yeast or Yersinia?   Currently, the ways its has been handed down for X years, is that we inoculate three Thio broth tubes and incubate them at 4C, 22C, and 35-37C for 5 days.   If there is any growth we, gram stain and sub to appropriate media plates.     I am a little concerned if this is still adequate, esp for Yersinia spp.   What does your Blood Bank Transfusion Service "think" you are screening for? 

Thanks

Link to comment
Share on other sites

  • 4 years later...

  • 5 months later...

If bacterial contamination is suspected due to the signs/symptoms of the patient(high fever, vomitting, tachycardia, etc..) and/or discoloration of the blood product, an initial gram stain is performed and 4 plates are inoculated with the blood, Blood agar, chocalate, mackonkey, and an anaerobic plate. Then we go from there.

 

Not sure about the reasoning of incubating the broth at 4 degrees C.  Is that to rule in/out a cold agglutinin? 

Link to comment
Share on other sites

11 hours ago, SbbPerson said:

If bacterial contamination is suspected due to the signs/symptoms of the patient(high fever, vomitting, tachycardia, etc..) and/or discoloration of the blood product, an initial gram stain is performed and 4 plates are inoculated with the blood, Blood agar, chocalate, mackonkey, and an anaerobic plate. Then we go from there.

I sincerely hope that you would also contact your blood supplier to let them know that there is a high risk of bacterial infection, as there may well be other blood components from the same donor that may yet still be untransfused to a patient, and which should be immediately put into quarantine.

Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
 Share

  • Advertisement

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.