Jump to content

Featured Replies

Posted
comment_70041

I am curious to find out what methods other Microbiology Labs are using for investigating Transfusion Reactions for the Blood Bank Transfusion Service?  Limited verbal communications suggest there are a lot of variations out there.   Based on product, how do you screen for Salmonella, etc., Staph aureus, Yeast or Yersinia?   Currently, the ways its has been handed down for X years, is that we inoculate three Thio broth tubes and incubate them at 4C, 22C, and 35-37C for 5 days.   If there is any growth we, gram stain and sub to appropriate media plates.     I am a little concerned if this is still adequate, esp for Yersinia spp.   What does your Blood Bank Transfusion Service "think" you are screening for? 

Thanks

  • 4 years later...
  • Replies 4
  • Views 3.9k
  • Created
  • Last Reply

Top Posters In This Topic

Most Popular Posts

  • Malcolm Needs
    Malcolm Needs

    I sincerely hope that you would also contact your blood supplier to let them know that there is a high risk of bacterial infection, as there may well be other blood components from the same donor that

  • donellda
    donellda

    If you have enough of the unit left, you could use blood culture vials along with an initial gram stain.

comment_83556

If you have enough of the unit left, you could use blood culture vials along with an initial gram stain.

  • 5 months later...
comment_84372

If bacterial contamination is suspected due to the signs/symptoms of the patient(high fever, vomitting, tachycardia, etc..) and/or discoloration of the blood product, an initial gram stain is performed and 4 plates are inoculated with the blood, Blood agar, chocalate, mackonkey, and an anaerobic plate. Then we go from there.

 

Not sure about the reasoning of incubating the broth at 4 degrees C.  Is that to rule in/out a cold agglutinin? 

comment_84376
11 hours ago, SbbPerson said:

If bacterial contamination is suspected due to the signs/symptoms of the patient(high fever, vomitting, tachycardia, etc..) and/or discoloration of the blood product, an initial gram stain is performed and 4 plates are inoculated with the blood, Blood agar, chocalate, mackonkey, and an anaerobic plate. Then we go from there.

I sincerely hope that you would also contact your blood supplier to let them know that there is a high risk of bacterial infection, as there may well be other blood components from the same donor that may yet still be untransfused to a patient, and which should be immediately put into quarantine.

  • 7 months later...
comment_85929

I believe the 4C thio is for isolation of Yersinia - likes the cold - but I haven't worked microbiology in many years now.  There's a special media micro uses for that now - I think.

 

Create an account or sign in to comment

Recently Browsing 0

  • No registered users viewing this page.

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.