Posted June 5, 20178 yr comment_70041 I am curious to find out what methods other Microbiology Labs are using for investigating Transfusion Reactions for the Blood Bank Transfusion Service? Limited verbal communications suggest there are a lot of variations out there. Based on product, how do you screen for Salmonella, etc., Staph aureus, Yeast or Yersinia? Currently, the ways its has been handed down for X years, is that we inoculate three Thio broth tubes and incubate them at 4C, 22C, and 35-37C for 5 days. If there is any growth we, gram stain and sub to appropriate media plates. I am a little concerned if this is still adequate, esp for Yersinia spp. What does your Blood Bank Transfusion Service "think" you are screening for? Thanks
May 31, 20223 yr comment_83556 If you have enough of the unit left, you could use blood culture vials along with an initial gram stain.
November 5, 20222 yr comment_84372 If bacterial contamination is suspected due to the signs/symptoms of the patient(high fever, vomitting, tachycardia, etc..) and/or discoloration of the blood product, an initial gram stain is performed and 4 plates are inoculated with the blood, Blood agar, chocalate, mackonkey, and an anaerobic plate. Then we go from there. Not sure about the reasoning of incubating the broth at 4 degrees C. Is that to rule in/out a cold agglutinin?
November 6, 20222 yr comment_84376 11 hours ago, SbbPerson said: If bacterial contamination is suspected due to the signs/symptoms of the patient(high fever, vomitting, tachycardia, etc..) and/or discoloration of the blood product, an initial gram stain is performed and 4 plates are inoculated with the blood, Blood agar, chocalate, mackonkey, and an anaerobic plate. Then we go from there. I sincerely hope that you would also contact your blood supplier to let them know that there is a high risk of bacterial infection, as there may well be other blood components from the same donor that may yet still be untransfused to a patient, and which should be immediately put into quarantine.
June 13, 20232 yr comment_85929 I believe the 4C thio is for isolation of Yersinia - likes the cold - but I haven't worked microbiology in many years now. There's a special media micro uses for that now - I think.
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