mrmic Posted June 5, 2017 Share Posted June 5, 2017 I am curious to find out what methods other Microbiology Labs are using for investigating Transfusion Reactions for the Blood Bank Transfusion Service? Limited verbal communications suggest there are a lot of variations out there. Based on product, how do you screen for Salmonella, etc., Staph aureus, Yeast or Yersinia? Currently, the ways its has been handed down for X years, is that we inoculate three Thio broth tubes and incubate them at 4C, 22C, and 35-37C for 5 days. If there is any growth we, gram stain and sub to appropriate media plates. I am a little concerned if this is still adequate, esp for Yersinia spp. What does your Blood Bank Transfusion Service "think" you are screening for? Thanks Link to comment Share on other sites More sharing options...
donellda Posted May 31, 2022 Share Posted May 31, 2022 If you have enough of the unit left, you could use blood culture vials along with an initial gram stain. SbbPerson and David Saikin 2 Link to comment Share on other sites More sharing options...
SbbPerson ★ Posted November 5, 2022 Share Posted November 5, 2022 If bacterial contamination is suspected due to the signs/symptoms of the patient(high fever, vomitting, tachycardia, etc..) and/or discoloration of the blood product, an initial gram stain is performed and 4 plates are inoculated with the blood, Blood agar, chocalate, mackonkey, and an anaerobic plate. Then we go from there. Not sure about the reasoning of incubating the broth at 4 degrees C. Is that to rule in/out a cold agglutinin? Link to comment Share on other sites More sharing options...
Malcolm Needs ★ Posted November 6, 2022 Share Posted November 6, 2022 11 hours ago, SbbPerson said: If bacterial contamination is suspected due to the signs/symptoms of the patient(high fever, vomitting, tachycardia, etc..) and/or discoloration of the blood product, an initial gram stain is performed and 4 plates are inoculated with the blood, Blood agar, chocalate, mackonkey, and an anaerobic plate. Then we go from there. I sincerely hope that you would also contact your blood supplier to let them know that there is a high risk of bacterial infection, as there may well be other blood components from the same donor that may yet still be untransfused to a patient, and which should be immediately put into quarantine. Ensis01 and SbbPerson 2 Link to comment Share on other sites More sharing options...
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