Immucor W.A.R.M.

[Liz 227]

I would like to receive the package insert of the WARM kit by Immucor.

Thank you

Liz

[L106 271]

Liz -

There's a way to go to Immucor's website (www.immucor.com), and print a copy of any of their package inserts. Once on the website, you have to click on USER GROUP and sign up to get access. (It's a little tricky.)

Donna

[Eagle Eye 203]

I will try to attach it here.:)

WARM.pdf

[Dr. Pepper 909]

Liz, if I can put in yet another plug for the PEG autoadsorption technique from the AABB Technical Manual: we switched to this technique after using WARM for several years. It is easier, quicker, more efficient, uses less cells, produces more adsorbed serum and is substantially cheaper.

[Eagle Eye 203]

Did you ever encounter a situation where antibody was diluted?

[Dr. Pepper 909]

We have found that 2 adsorptions are usually sufficient to get rid of all the autantibody, as compared to 3 or 4 using WARM. So there is less dilutional effect from residual saline in the red cells. As for the procedural dilution of the test serum by adding an equal volume of PEG, that's pretty much what happens if you were using PEG on unadsorbed serum. The Tech Manual notes that some have reported a weakening of antibody reactivity compared to other methods, and that some use 6 drops of adsorbed serum/PEG mixture instead of 4. We tested several spiked samples using PEG vs. WARM - adsorbed sera. We found equal or higher titers with the PEG sera (6 drops) and feel quite comfortable with the technique.

If you know where you're headed, you can do a serum panel, DATs, eluate and panel, autoadsorptions and crossmatches and get the blood out the door in less than 2 hours.

[David Saikin 1,353]

Dr Pepper speaks the truth . . .

[Liz 227]

Thank you all very much for your kind help.

Liz

[Liz 227]

I have a question that I didn't find in the insert, and based on your experience: how many vials are there in a WARM kit and approximately how many autoantibody removals can I perform per vial. (I need this information to justify havng the WARM kits on the Blood Bank Budget).

Thank you

Liz

[Eagle Eye 203]

I believe 10 vials/kit. And as I said earlier in my post, if you are doing two cycle you will use one vial. once you make reagent it's basically good for one patient.

If your patient population is as such where you get DAT 1+ ot +/-...I think two cycle would be enough so in that case 10 patient/kit.

[Liz 227]

Thank you very much.

Liz:flirty:

[Liz 227]

Liz, if I can put in yet another plug for the PEG autoadsorption technique from the AABB Technical Manual: we switched to this technique after using WARM for several years. It is easier, quicker, more efficient, uses less cells, produces more adsorbed serum and is substantially cheaper.

Is PeG from Immucor?

Do you have the Package Insert?

Thank you,

Liz

[ABIDer 11]

Has anyone ever done a Ficin Adsorption? If so, what are your thoughts on it?

[David Saikin 1,353]

Ficin pretreatment autoabsorption is very tedious, labor intensive and time consuming. It used to be the norm . . . now I do PeG autoabsorptions.

[Liz 227]

Is PeG from Immucor?

Do you have the Package Insert?

Thank you,

Liz

I have finally reached the comany I recieved the info.

thanks

Liz :D:D

[applejw1 27]

Has anyone ever had patient plasma coagulate after 2x W.A.R.M autoadsorption?  Specimen was stored at room temperature and looked gelatinous after overnite.