ChrisH

Looking for a reference to doing Reverse typing using IgG testing. We have a A Pos patient (historical) that now has a very strong cold that we can not get rid of it. So we sent it to our reference lab. They cleared up the ABO discrepancy on the reverse by doing the testing at IgG.

I am revisiting this question. Does Irradiating a product be considered preparing?

We use their A1 cells, B cells, Check cells (and yes they are a little weaker), Anti-A, B, D, AB and a few rare antisera.

I also would like to know if anyone is validating each shipment/lot. I am currently doing my initial validation. Just trying to plan what else I need to do and how often.

We have used them for a few years. A lot easier to clean up the sand then glycerol.

We also do this, we get it from Fisher. We buy a new one when it expires https://www.fishersci.com/shop/products/fisher-scientific-traceable-big-digit-type-k-thermometer/15078187#?keyword=Type-K+Digital+Thermometer+Probes+Type-K+Beaded+Probe

But we are seeing anti-K and anti-E in gel; so is it the genetics of these antibodies or is it that they are so weak that gel just does not pick them up?

Hi everyone, We have seen some patients that are demonstrating anti-K with PEG only. We repeat with Gel and tube LISS and results are negative. We change our incubation times and still negative, we have used K homologous cells in gel and still negative. Anyone have any hints as to why this is happening. Chris

We do NOT want to irradiate our own products. We were asked to look into the pricing. We keep irradiated RBC, but the Plt are not and most of the time it takes to long to get the product from our blood supplier.

Currently our cancer center uses their linear accelerators to irradiate our products, but they want us to investigate another methods. In the last 2 years we have irradiated less than 340 products per year. So I think getting an irradiator would be to cost prohibitive. But I still need to know what is out there. I have seen Nordion mentioned, but when I go to their site I can not find a blood irradiator.

For those doing QC, Say you use a Fya to QC 1) Are you QC'ing all 11 cells, or selected positive and negative cells? 2) If you have more then one panel / method are you QC them also? We are not QC'ing panels yet, we have Ortho C (regular .8% and ficin), and B panels, and a tube panel. We have a single tech on dayshift, one on second and third shift is a Hemo/BB tech. I am trying to figure out how we would do this QC daily, and not use up the cells before the panels expired.

As of right now we do not QC our panels, we have both Gel and Tube panels and we do QC out gel and tube screening cells. I went through our different manufactures panel IFU I have the following questions and thoughts. Ortho says "should be tested periodically with weak antibodies" 1) What is periodically? Defined as repeatedly at regular time. Is this once for each panel? or one a day, week, month or year? Or what? 2) Weak, how weak 1+? 3) Antibodies - how many, all of them? Only clinically significant or what? Bio-Rad says "Suitable controls should be included in each test run." 1) Each test run? Meaning with each panel run? That could be a couple times a day.... Immucor - Says “the reactivity of the red blood cells may be checked periodically by testing antigens likely to deteriorate, such as Lea, with a weakly reactive antibody of the same specificity. Still says periodically but is more specific about the antibody. Quotient-Alba says "Quality Control is essential and should be performed in accordance with local, state and federal regulations. This one is wide open

check out this topic http://www.pathlabtalk.com/forum/index.php?/topic/7433-new-ortho-mts-workstaion/?hl=ortho

GCH Lab Rat and David, thank you I found them in the Fisher Scientific catalog...

I agree Uggh! We started the Fetal Bleed lot to lot this summer but did not do the Elu kit. The only thing we have that would be positive is the Survey and that does not give us enough to do twice in tube.... I would say all most all of the patients we do are all positive or all negative and we only do about 10 a year.... may be better to send these patients to the reference lab, but then out turn around time will stink..

Anyone out there know of any 600ml single bags with a male adapter (not a spike) on the tubing? We do between 5 -10 a year and that is it.

We also are going to start to use a second sample Another question. What do you do if someone goes to OR from the ER and no second sample? Would this be emergency release?

We are a fairly small hospital, with only one full time BB Tech (me), we do one audit a month.

We have 2 ways that are not paperless. 1) We have an emergency release when the testing is not done. 2) For those units that are least incomparable we have a exception form that the doctor needs to sign. Otherwise everything is paperless.

Many years ago we had a patient with a Vel, she would hemolyze any non Vel positive cells on the panel. We also had problems find blood for her and we had her donate some units which we froze. i would what happened to her.

David, what is the website?

The whole lab except for BB was updated to W7, our version on HBB is not validated for W7. But I do have a W7 with HBB on it that I can test and there are a few problems. Also our IT guys had to leave a few older XPs around because of what software/programs were on them and we are slowly moving stuff off.

I have been told that we have to do KB because of turn around time. The OB/ER want to know now and do not want to wait a day or so for the flow results...

What they do not say is that the new Cent-Incub combo takes up more counter space then the old (since the old incubator sits on top of the centrifuge).

We have our inpatient criteria set, but now are working on our outpatient transfusion criteria. Is anyone willing to share their criteria? Do anyone know of any current reading material about this? I have found a few but most are over 10 years of age.