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April 2024 Challenge

Yanxia

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Everything posted by Yanxia

  1. Yanxia
    it is a long post to me the first one, i often see B antigens are weaker than A antigens on our newborns, but ont as weak as 1+, i think it maybe an ABsubgroup. the second one,"Lutheran antibodies have not been implicated in immediate haemolytic transfusion reactions, although they may have been responsible for mild delayed reactions and post-transfusion jaundice."I think the symptom after transfusion fit it. Geoff Daniel, Human bloog groups,second edition, 279,230
  2. Yanxia
    Sir, do you mean the anti-A1 in the A2 individual will destruct the transfused A1 cells where the circulation temperature is lower than 37oc, even the anti-A1 has no reaction at 37oc
  3. Yanxia
    the anti-A1 idoes rarely react at 37 degree Celsius, but the anti-A or/and anti-B, anti-AB do.
  4. Yanxia
    just my personal opinion, when the antigens and antibodies reaction, there is a formula, in this case, the antigens( the binding antibodies on the cells) is fewer,( we can see this from the reaction strength w+), so add more antigens will give stronger reaction. of course it is in a range.
  5. Yanxia
    first of all, i have not done this test before.my opinion is just from my understanding of the threats i read before, just to express myself, myabe and most likely it is not right. the auto control is the reaction of patient's cells with its own plasma, the DTT treatment is for the screening cells, if i understand it rightly. so the DTT does not do anything to the autocells.
  6. Yanxia
    i guess the difference is because the second time's cells is more than the first time( two drops vs. one)
  7. Yanxia
    we will not transfuse in this situation
  8. Yanxia
    i am sorry if my understanding is wrong. Do you mean if the salin panel result is neg, you will not do adsorption test even the enzyme and PEG/LISS panel results is pos? I think it is not safe, because most allo -antibodies are IgG, they are non reactive in saline.
  9. Yanxia
  10. Yanxia
    and from my daily work, i find we can tell from the proportion of cells, if transfused is less, then on the mix field result, the large part is the patient's own cells
  11. Yanxia
    there is a method which using a microtube( sorry, i am not sure how to call it), fill the tube with blood, then centrifuge it, the new generated red cellls are lighter so they are on the upper layer, they are the patients' own cells, the transfused cells are heavier, so they are on the bottom
  12. Yanxia
    It seems a weak anti-D which is sometimes too weak to show up on enzyme technique. Maybe the former several pregnancies immune the lady.Maybe knowing the kids D type will help.
  13. Yanxia
    i cannot remember we do in-dated panel cells QC in the reference lab use antiserum . Just as Debbiel mentioned the panel cells must be visually qualified. we use them to do antibodies identification, then get the result, use reagent antiserum to confirm the antigen is absence on the auto cells if DAT is neg( if we have those reagent), then use two antigen pos cells and two antigen neg cells to confirm the antibodies again. the panel cells express a lot of antigens , some of them are rare and it is hard to get the specific antiserum, i think it is hard to QC those antigens during the life span of the panel.
  14. Yanxia
    The patient is AB, I don't think the transfused Rbcs are the problem. Maybe because the A platelets with high titer of anti-B or the patient's blood volume is smaller than normal adult. I think do an elution is good to prove what is on the red cells, there are lots of ABO HDFN has neg DAT, but pos elution.
  15. Yanxia
    The patient is AB, I don't think the transfused Rbcs are the problem. Maybe because the A platelets with high titer of anti-B or the patient's blood volume is smaller than normal adult. I think do an elution is good to prove what is on the red cells, there are lots of ABO HDFN has neg DAT, but pos elution.
  16. Yanxia
    Maybe the anti-B is just room temperature reactive, the transfusion is safe. And what if he or she receive an AB donor kidney...
  17. Yanxia
    Thanks again Brenda for sharing this case with us. I just wonder if at first we use the Erytra (Automated Grifols GEL) , it gives a good result as AB, then this patient received AB red cells, what will happen?
  18. Yanxia

    Happy birthday, my dear teacher

    Thanks for the knowledge you shared with us, I like to read your posts very much. There had less  professional teaching about blood bank professional knowlege  in school in my country. They have it now. For me, I learn my professional knowledge mainly through internet, from  great teachers like you.

    I am sorry for the late wish, I just back from my hometown, there has no internet.

    best wishes

    Shily

    1. Malcolm Needs

      Malcolm Needs

      Thank you shily.

  19. Yanxia
    If the reverse typing use the same A and B cells, then the differ between gel and tube are caused by the methods, otherwise, it maybe caused by low antibodies against B cells in tube method. To verify it, we can change to another B cells to test in tube method. I tend to agree it looks like an ABsubgroup, on my daily use of gel, I find it is not as sensitive to detect reverse reaction as tube method, I guess that is the cause of no reverse reaction on gel but has reaction in tube.
  20. Yanxia
    I guess the antibodies come from the donors. And the antobodies are against low infrequency antigens on the recipient's red cells, so" the eluate did not react with the transfused RBC...and the panel cells..."
  21. Yanxia
    enzyme treatment may enhance the Rh system antibodies reaction, maybe it can help
  22. Yanxia

    Thank you very much, sir. I am sorry for the late reply, because there was something went wrong on my computer, I just fixed it today.

    My best wishes

    shily

  23. Yanxia
    I think this is a very good question. We have encountered several DCT+ donors, the bloods have been returnted to blood center. I just wonder whether it is significant of the donor DCT+ , anyway they are eligible to donate.
  24. Yanxia
    Have not been transfused, and DAT is pos. If this anti-c can be absorbed out by his own cells, then it is auto antibody.
  25. Yanxia
    you are welcome
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